Soil moisture (SM) datasets at high spatial resolutions are beneficial for a wide range of applications, such as monitoring and prediction of hydrological extremes, numerical weather prediction, and precision agriculture. For large scale applications in particular, remotely sensed SM has advantages over in situ data because it provides gridded estimates and because it is less labour-intensive. However, until present, active microwave SM data have not been presented at their native spatial resolution, since the quality of these data is limited by speckle. We explored the potential and limits of high spatial resolution of active microwave SM observations. We used a Sentinel-1 C-band SAR SM dataset at six spatial resolutions ranging from 20 × 20 to 120 × 120 m2. This was compared to a closely spaced (20 m) in situ dataset collected on a non-irrigated agricultural field (±2.5 ha) in the Southeast of Luxembourg. A comparison of the field and satellite datasets demonstrated how Sentinel-1 data with a high spatial resolution can be used to quantify temporal within-field SM variability. SM was accurately estimated at spatial resolutions of 60 × 60 m2 and coarser, where the temporal correlation was found to be 0.67 and sub-field variations in SM were still detected. Spatial correlation was limited by the absence of SM variability within the field. These results indicate that high spatial resolution SM estimates from Sentinel-1 data can be valuable for monitoring temporal SM variations within agricultural fields. •Limits in spatial resolution were tested in Sentinel-1 soil moisture retrievals.•Spatiotemporal variability was evaluated using closely spaced field reference data.•The optimal spatial resolution is 60 × 60 m2 with a temporal correlation of 0.67.•Temporal soil moisture variability could be retrieved accurately within the field.