A new methodology based on the fluorescence of Cu(II) ternary system with o-phenanthroline (o-Phen) and eosin (Eo) dyes is proposed. The metal was selectively retained on Nylon membranes and the solid surface fluorescence (SSF) was used for anayte quantification. Experimental variables that influence the formation of Cu(II)-o-Phen-eo system and retention step were studied and optimized. At optimal experimental conditions, an adequate tolerance to foreign species was shown with a LOD of 1.18 ng L −1 and a LOQ of 3.57 ng L −1 . The methodology was evaluated for their greenness profile and successfully applied to analyte determination in bee’s products of West-Center Argentina. Recovery studies showed values near to 100% being satisfactorily validated by ICP-MS.