Expression of virally transduced mouse tyrosinase in tyrosinase-negative chick embryo melanocytes in culture

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Expression of virally transduced mouse tyrosinase in tyrosinase-negative chick embryo melanocytes in culture

Whitaker, B A; Frew, T J; Greenhouse, J J; Hughes, S H; Yamamoto, H; Takeuchi, T; Brumbaugh, J A

Pigment cell research, November 1989, Volume: 2, Issue: 6

Journal Article

A cDNA encoding mouse tyrosinase was inserted into a plasmid containing the provirus of a replication competent avian leukosis virus (ALV). A viral stock produced from the plasmid was used to infect cultured tyrosinase-negative (ca/ca) unpigmented chick embryo melanocytes. Five days after infection many cells were producing very dark discrete melanosomes.

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