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  • Polarimetric second-harmoni...
    Golaraei, Ahmad; Mostaço-Guidolin, Leila B; Raja, Vaishnavi; Navab, Roya; Wang, Tao; Sakashita, Shingo; Yasufuku, Kazuhiro; Tsao, Ming-Sound; Wilson, Brian C; Barzda, Virginijus

    Biomedical optics express, 04/2020, Volume: 11, Issue: 4
    Journal Article

    Polarimetric second-harmonic generation (P-SHG) microscopy is used to quantify the structural alteration of collagen in stage-I,-II and -III non-small cell lung carcinoma (NSCLC) tissue. The achiral and chiral molecular second-order susceptibility tensor components ratios ( and , respectively), the degree of linear polarization ( ) and the in-plane collagen fiber orientation ( ) were extracted. Further, texture analysis was performed on the SHG intensity, , , and . The distributions of , , and as well as the textural features of entropy, correlation and contrast show significant differences between normal and tumor tissues.