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Wolberger, Cynthia; Wang, Tao; Zhang, Xiangbin; Bheda, Poonam; Revollo, Javier R; Imai, Shin-ichiro
Nature structural & molecular biology, 07/2006, Volume: 13, Issue: 7Journal Article
Nicotinamide phosphoribosyltransferase (Nampt) synthesizes nicotinamide mononucleotide (NMN) from nicotinamide in a mammalian NAD+ biosynthetic pathway and is required for SirT1 activity in vivo. Nampt has also been presumed to be a cytokine (PBEF) or a hormone (visfatin). The crystal structure of Nampt in the presence and absence of NMN shows that Nampt is a dimeric type II phosphoribosyltransferase and provides insights into the enzymatic mechanism.
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