Environmental safety has become a significant issue for the safety of living species, humans, and the ecosystem as a consequence of the harmful and detrimental consequences of various pollutants such as pesticides, heavy metals, dyes, etc., emitted into the surroundings. To resolve this issue, various efforts, legal acts, scientific and technological perspectives have been embraced, but still remain a global concern. Furthermore, due to non-portability, complex detection, and inappropriate on-site recognition of sophisticated laboratory tools, the real-time analysis of these environmental contaminants has been limited. As a result of innovative nano bioconjugation and nanofabrication techniques, nanotechnology enables enhanced nanomaterials (NMs) based (bio)sensors demonstrating ultra-sensitivity and a short detection time in real-time analysis, as well as superior sensitivity, reliability, and selectivity have been developed. Several researchers have demonstrated the potent detection of pollutants such as Hg2+ ion by the usage of AgNP-MD in electronic and optoelectronic methods with a detection limit of 5–45 μM which is quite significant. Taking into consideration of such tremendous research, herein, the authors have highlighted 21st-century strategies towards NMs based biosensor technology for pollutants detection, including nano biosensors, enzyme-based biosensors, electrochemical-based biosensors, carbon-based biosensors and optical biosensors for on-site identification and detection of target analytes. This article will provide a brief overview of the significance of utilizing NMs-based biosensors for the detection of a diverse array of hazardous pollutants, and a thorough understanding of the detection processes of NMs-based biosensors, as well as the limit of quantification (LOQ) and limit of detection (LOD) values, rendering researchers to focus on the world's need for a sustainable earth. Display omitted •A recent analysis on the rapid detection of pollutants has been presented.•Nanomaterials-based biosensors plays a crucial role in the detection of pollutants.•Biosensors exhibited a viable alternative to establish effective analytical methods.•Nanomaterials-based biosensors exhibit enhanced sensitivity and reliability.