•Negative Poisson's ratio jounce bumper (NPRJB) was assembled into suspension and vehicle models in Adams/Car.•NPRJB achieved better suspension stiffness curve without adjusting free travel.•NPRJB had considerable positive influences on the vehicle ride comfort travelling through bump. Comparing with traditional honeycomb structures, Negative Poisson's Ratio (NPR) structures had better mechanical performances in some certain respects, especially the shear modulus and fracture toughness. However, few publications focused on the cylinder-shape NPR structure, which influence the diversity and possibility of NPR structure applications. In this paper, a cylindrical NPR structure was introduced and applied as a suspension jounce bumper in order to solve the issue that the ideal uniaxial compression load-displacement curve sometimes cannot be realized by traditional Polyurethane (PU) jounce bumper. The load-displacement curve of NPR jounce bumper was proved to be smoother and more ideal than that of traditional jounce bumper. Nevertheless, the influences of NPR jounce bumper on the suspension mechanical performance and vehicle ride comfort were not comprehended yet. In this study, the traditional and NPR jounce bumpers were both assembled into virtual prototypes of Macpherson, double wishbone and multi-link suspensions to conduct single wheel travel virtual tests. The results indicated that NPR jounce bumper can achieve more ideal wheel force vs. jounce height curve without adjusting free travel, which is beneficial to spare precise suspension space. Furthermore, a jounce bumper evaluation method using pulse ride comfort was proposed in this paper. The virtual ride comfort tests of travelling through bump and pothole were conducted using established vehicle virtual prototype. The maximum vertical accelerations and weighted root mean square (RMS) of acceleration of vehicle centroid at most speeds were reduced applying NPR jounce bumper. Thus, the NPR jounce bumper can apparently improve vehicle ride comfort.