E-resources
Peer reviewed
-
Mestdagh, Pieter; Hartmann, Nicole; Baeriswyl, Lukas; Andreasen, Ditte; Bernard, Nathalie; Chen, Caifu; Cheo, David; D'Andrade, Petula; DeMayo, Mike; Dennis, Lucas; Derveaux, Stefaan; Feng, Yun; Fulmer-Smentek, Stephanie; Gerstmayer, Bernhard; Gouffon, Julia; Grimley, Chris; Lader, Eric; Lee, Kathy Y; Luo, Shujun; Mouritzen, Peter; Narayanan, Aishwarya; Patel, Sunali; Peiffer, Sabine; Rüberg, Silvia; Schroth, Gary; Schuster, Dave; Shaffer, Jonathan M; Shelton, Elliot J; Silveria, Scott; Ulmanella, Umberto; Veeramachaneni, Vamsi; Staedtler, Frank; Peters, Thomas; Guettouche, Toumy; Wong, Linda; Vandesompele, Jo
Nature methods, 08/2014, Volume: 11, Issue: 8Journal Article
MicroRNAs are important negative regulators of protein-coding gene expression and have been studied intensively over the past years. Several measurement platforms have been developed to determine relative miRNA abundance in biological samples using different technologies such as small RNA sequencing, reverse transcription-quantitative PCR (RT-qPCR) and (microarray) hybridization. In this study, we systematically compared 12 commercially available platforms for analysis of microRNA expression. We measured an identical set of 20 standardized positive and negative control samples, including human universal reference RNA, human brain RNA and titrations thereof, human serum samples and synthetic spikes from microRNA family members with varying homology. We developed robust quality metrics to objectively assess platform performance in terms of reproducibility, sensitivity, accuracy, specificity and concordance of differential expression. The results indicate that each method has its strengths and weaknesses, which help to guide informed selection of a quantitative microRNA gene expression platform for particular study goals.
Author
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.