This study comparatively evaluated the effects of different extraction methods on yield, structural features and antioxidant activities of Laminaria japonica (L. japonica) polysaccharides. Results showed that acid, water and alkaline extracted L. japonica polysaccharides (LJP-CA, LJP-W and LJP-A, respectively) differed significantly. Among three polysaccharides, LJP-W showed the highest viscosity with high uronic acid content and average molecular weight, whereas LJP-CA showed the lowest viscosity with low uronic acid and high sulfate content with moderate average molecular weight. LJP-CA was mainly composed of fucose, mannose and galactose, but the proportion of glucose was greatly increased in LJP-W and LJP-A. LJP-W had an even and smooth sheet-like appearance, while LJP-CA and LJP-A exhibited irregular and rough fragments or particles with stronger antioxidant activities. These results suggest that acid and alkali would propose an improved process for polysaccharide preparation from L. japonica and to exploit its potential application as a functional ingredient in food application. •Laminaria japonica polysaccharide was extracted by acidic, water and alkaline method.•The yield of alkaline extraction (LJP-A) was the highest.•LJP-W had the highest uronic acid content, Mw, and viscosity.•CD and SEM showed the difference in surface and conformational characteristics.•LJP-CA presented the highest superoxide and hydroxyl radical scavenging activities.