A BSTRACT Aim: The aim of this research was to identify the antimicrobial effectiveness of three different mouthwashes on periodontal pathogenic microorganisms. Materials and Methods: 2 periodontal disease-causing microorganisms, i.e., Aggregatibacter actinomycetemcomitans , and Porphyromonas gingivalis , were chosen for this investigation. Prior to commencing this research, a variety of branded and commercially obtainable mouthwashes were procured. Three oral rinses, namely HiOra, Hexidine, and Amflor, were chosen for the current research. The subculture of A. actinomycetemcomitans as well as P. gingivalis was performed by subjecting them to incubation for 48 to 72 hours at 35–37°C. The disk diffusion method was employed to evaluate the antibacterial efficiency of the extract in opposition to the pathogens tested. The zone of inhibition was calculated in millimeters. The mean value of every sample was documented. Results: Hexidine oral rinse in pursuit by Amflor as well as HiOra oral rinse exhibited the highest zone of inhibition in opposition to A . Actinomycetemcomitans and P. gingivalis . The differences amid the groups were statistically significant with a P value < 0.001. Conclusion: The current research concluded that amid the three different oral rinses employed in the current research, Hexidine oral rinse exhibited greatest antimicrobial effectiveness versus Amflor and HiOra mouthrinse.