To introduce testing of Grapevine leafroll-associated virus-2 (GLRaV-2) and Grapevine virus B (GVB) in sanitary selection of grapevine, commercially available antibodies were evaluated and conditions for routine ELISA testing were optimized. Extraction procedure with Granex 91 - special machine, which is used in routine testing in Slovenia, was compared with grinding samples in mortar. Three different extraction buffers were applied in order to overcome the inconvenience of using more than one extraction procedure when testing grapevine material for several viruses in a routine large-scale testing scheme. Results were verified with Western blot and immuno-electron microscopy. The best results were obtained using extraction buffer with unknown composition (pH 9.0) from BIOREBA kit for GLRaV-2. Other extraction buffers gave less positive samples and they are not convenient for routine testing where extraction with Granex is done. Both viruses, GLRaV-2 and GVB were found in Slovenia, but they couldn't be correlated with rougose wood disease that appears on indigenous cultivar Refošk grafted on ‘SO4’ from collection vineyard in Komen. Z namenom, da bi uvedli testiranje virusa Grapevine leafroll-associated virus-2 (GLRaV-2) in virusa Grapevine virus B (GVB) v zdravstveno selekcijo vinske trte, smo testirali komercialno dostopna protitelesa in optimizirali pogoje za rutinsko testiranje v ELISA. Primerjali smo ekstrakcijo s strojem Granex 91, ki ga v Sloveniji uporabljajo v rutinskem testiranju, z ekstrakcijo v terilnici. Da bi poenotili ekstrakcijo različnih virusov v obsežnem rutinskem testiranju, smo preizkusili tri različne ekstrakcijske pufre. Rezultate smo preverili z imunskim pivnikom (Western blot) in imunsko elektronsko mikroskopijo. Najboljše rezultate smo dobili pri vzorcih ekstrahiranih s pufrom nepoznane sestave iz kita za določevanje GLRaV-2 proizvajalca BIOREBA. Z ostalima pufroma smo dobili manj pozitivnih vzorcev, zato menimo, da pufra nista primerna za ekstrakcijo vzorcev v rutinskem testiranju kjer se uporablja stroj Granex 91. Ugotovili smo prisotnost obeh virusov v Sloveniji, nismo pa uspeli dokazati povezave teh dveh virusov z razbrazdanjem lesa, ki se pojavlja na trsih domače sorte Refošk cepljenih na podlago ‘SO4’ iz kolekcijskega vinograda v Komnu.