E-resources
-
Cavalloro, Valeria; Marrubini, Giorgio; Stabile, Rita; Rossi, Daniela; Linciano, Pasquale; Gheza, Gabriele; Assini, Silvia; Martino, Emanuela; Collina, Simona
Molecules (Basel, Switzerland), 01/2021, Volume: 26, Issue: 2Journal Article
During the years, many usnic acid (UA) conjugates have been synthesized to obtain potent endowed with biological properties. Since ( )-UA is less abundant in nature than ( )-enantiomer, it is difficult to source, thus precluding a deeper investigation. Among the lichens producing UA, is a valuable (S)-UA source. In the present work, we report on a rapid HPLC-UV/PAD-CD protocol suitable for the analysis and the identification of the main secondary metabolites present in extract. Best results were achieved using XBridge Phenyl column and acetonitrile and water, which were both added with formic acid as mobile phase in gradient elution. By combining analytical, spectroscopical, and chiroptical analysis, the most abundant analyte was unambiguously identified as ( )-UA. Accordingly, a versatile microwave-assisted extractive (MAE) protocol, assisted by a design of experiment (DoE), to quantitatively recover ( )-UA was set up. The best result in terms of UA extraction yield was obtained using ethanol and heating at 80 °C under microwave irradiation for 5 min. Starting from 100 g of dried , 420 mg of ( )-UA were achieved. Thus, our extraction method resulted in a suitable protocol to produce ( )-UA from for biological and pharmaceutical investigation or commercial purposes.
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.