ABSTRACT The present work is related to the preparation and characterization of biodegradable flexible films from macáçar bean starch, plasticized with glycerol and the glycerol and glacial acetic acid (HAc) mixture. The films were obtained by casting using macáçar bean starch (3 g/100 g of film-forming solution) and different concentrations of the glycerol plasticizing agent (20 g and 30 g/100 g of starch) and the glycerol and glacial acetic acid mixture, in the proportions 20 g and 10 g/100 g of film-forming solution, respectively. Water and glycerol as plasticizers affect mechanical and water vapor barrier properties of an edible wheat gluten film. Edible composite filme of wheat gluten and lipids water vapor permeability and other physical properties.