It was the aim of the present systematic review and meta-analysis to investigate the effects of high protein (HP) versus normal/low protein (LP/NP) diets on parameters of renal function in subjects without chronic kidney disease. Queries of literature were performed using the electronic databases MEDLINE, EMBASE, and the Cochrane Trial Register until 27th February 2014. Study specific weighted mean differences (MD) were pooled using a random effect model by the Cochrane software package Review Manager 5.1. 30 studies including 2160 subjects met the objectives and were included in the meta-analyses. HP regimens resulted in a significantly more pronounced increase in glomerular filtration rate MD: 7.18 ml/min/1.73 m2, 95% CI 4.45 to 9.91, p<0.001, serum urea MD: 1.75 mmol/l, 95% CI 1.13 to 237, p<0.001, and urinary calcium excretion MD: 25.43 mg/24h, 95% CI 13.62 to 37.24, p<0.001 when compared to the respective LP/NP protocol. HP diets were associated with increased GFR, serum urea, urinary calcium excretion, and serum concentrations of uric acid. In the light of the high risk of kidney disease among obese, weight reduction programs recommending HP diets especially from animal sources should be handled with caution.