Immobilization of enzymes (IoE) from animal origin on natural carriers increases the system stability; facilitates the separation and accelerates the recovery of the enzyme; makes reuse possible; provides a significant reduction in operating costs. There are numerous IoE methods and systems, including immobilization of various lipases on major carbohydrate biopolymers (chitin, chitosan, cellulose, etc.), discussed in this review. The key points of the most encouraging methods “for increasing the activity and stability” of such biopolymer systems are the “chitosan particle activation” by “ultra-sonication” and multiplicative “addition of glutaraldehyde” to these abovementioned systems. The design of such complex biopolymer preparations (in their various forms) is an important area of modern agrosciences, biomedicine, veterinary, zootechnology and bionanotechnology.