ABSTRACTObjectives: The study was aimed at isolating and identifying violacein-producing bacteria from the different ecosystems.Methods: The violet color pigment (violacein) producing bacteria was isolated and identified morphologically and genomically.Results: A total of ten bacterial Chromobacterium sp. were isolated from different sources such as soil and water. The violacein productivity wasscreened by the total isolates and the selected two strains were identified as Chromobacterium violaceum CV4 and Chromobacterium vaccinii CV5 bymorphologically and genomically.Conclusion: In the present study, the two isolate was morphologically and genomically identified as Chromobacterium violaceum CV4 andChromobacterium vaccinii CV5, last one was found to be the novel strain from natural sources which possess a high violacein producing capability.Keywords: Chromobacterium sp., Violacein, Pigment production.