E-resources
Peer reviewed
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Bai, Wei; Spivak, David A.
Angewandte Chemie (International ed.), February 17, 2014, Volume: 53, Issue: 8Journal Article
The detection of viruses is of interest for a number of fields including biomedicine, environmental science, and biosecurity. Of particular interest are methods that do not require expensive equipment or trained personnel, especially if the results can be read by the naked eye. A new “double imprinting” method was developed whereby a virus‐bioimprinted hydrogel is further micromolded into a diffraction grating sensor by using imprint‐lithography techniques to give a “Molecularly Imprinted Polymer Gel Laser Diffraction Sensor” (MIP‐GLaDiS). A simple laser transmission apparatus was used to measure diffraction, and the system can read by the naked eye to detect the Apple Stem Pitting Virus (ASPV) at concentrations as low as 10 ng mL−1, thus setting the limit of detection of these hydrogels as low as other antigen‐binding methods such as ELISA or fluorescence‐tag systems. Eye see it: A strategy for the naked‐eye detection of target compounds by using shrink‐responsive hydrogels is presented. A “double imprinting” method was used, in which a virus‐bioimprinted hydrogel is further micromolded into a diffraction‐grating sensor by using imprint‐lithography techniques. The bioimprinting was performed with an impure extract, which eliminates the need for a pure template.
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