MYB transcription factors play important roles in plant responses to biotic and abiotic stress. In this study, , a R2R3-MYB gene, was cloned from wheat ( L.). TaODORANT1 was localized in the nucleus and functioned as a transcriptional activator. was up-regulated in wheat under PEG6000, NaCl, ABA, and H O treatments. -overexpressing transgenic tobacco plants exhibited higher relative water content and lower water loss rate under drought stress, as well as lower Na accumulation in leaves under salt stress. The transgenic plants showed higher CAT activity but lower ion leakage, H O and malondialdehyde contents under drought and salt stresses. Besides, the transgenic plants also exhibited higher SOD activity under drought stress. Our results also revealed that overexpression up-regulated the expression of several ROS- and stress-related genes in response to both drought and salt stresses, thus enhancing transgenic tobacco plants tolerance. Our studies demonstrate that TaODORANT1 positively regulates plant tolerance to drought and salt stresses.