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  • Method validation and measurement uncertainty evaluation for measurement of mass concentration of organic acids in fermentation broths by using ion chromatography [Elektronski vir]
    Drolc, Andreja ; Pintar, Albin
    In the present paper, a methodology for method validation and measurement uncertainty evaluation for the measurement of mass concentration of organic acids in fermentation broths was developed. ... Acetic acid was selected as a representative of organic acids. A detailed procedure for in-house method validation based on simple experimental design and consistent statistics is presented. In addition, a step-by-step illustration of ĆBottom-UpĆ approach for measurement uncertainty evaluation of acetic acid in fermentation broths is also provided. The major sources of uncertainty of the result of measurement were identified and the combined uncertainty was calculated. Our analytical protocol allowed us to quantify acetic acid in fermentation broths in mass concentrations up to 75 g L-1 with satisfactory recovery (102.3%) and repeatability lower than 2%. We also estimated within-laboratory reproducibility over 3-month period, which was 2.3%. We proved that the methodwas selective for the measurement of mass concentration of acetic acid in fermentation broths. Measurement uncertainty of results was evaluated to be6.2% with 95% confidence level. After validation and measurement uncertaintyevaluation steps, results obtained showed that the method can be applied to efficiently monitor fermentation processes.
    Vir: Accreditation and quality assurance : journal for quality, comparability and reliability in chemical measurement. - ISSN 0949-1775 (Vol. 17, no. 3, 2012, str. 323-330)
    Vrsta gradiva - e-članek
    Leto - 2012
    Jezik - angleški
    COBISS.SI-ID - 4851994

    Povezava(-e):

    http://www.springerlink.com/content/t7l347334h0r0255/
    DOI

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