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MF, Centralna medicinska knjižnica, Ljubljana (CMK)
Poletni urnik (1.7. do 31.8.2024): ponedeljek do četrtek: 9.00-15.00, petek: 9.00-13.00.
26.7., 15.8. in 16.8. 2024 bo knjižnica ZAPRTA.
  • Comparative analysis of DNA preservation in permanent and deciduous teeth of adults and non-adults [Elektronski vir] : implications for archaeological and forensic research
    Leskovar, Tamara ; Zupanič-Pajnič, Irena
    This study investigates the preservation of DNA in different categories of teeth, including permanent and deciduous, fully developed and not fully developed, in both adults and non-adults. Teeth were ... sampled from a modern-era cemetery in Ljubljana, Slovenia. DNA extraction was performed using a full demineralisation protocol. DNA quantity and quality were assessed using qPCR analyses, and autosomal STR typing was conducted to verify genetic profiles. Results revealed significant differences in DNA preservation among various tooth categories. Fully developed permanent teeth of adults exhibited the highest DNA yields, attributed to their fully developed roots and thicker cementum, which is rich in DNA. Deciduous teeth, with thinner enamel and cementum, showed lower DNA preservation regardless of developmental stage. Non-adult teeth generally yielded less DNA compared to adults, even when considering only fully developed permanent teeth, indicating factors beyond developmental stage. These findings suggest that, in archaeological and forensic contexts, researchers should prioritize fully developed permanent teeth for DNA analysis due to their superior preservation. Additionally, this study underscores the importance of considering tooth type and developmental stage when selecting samples for genetic analysis in cases where petrous bone is unavailable, expanding our understanding of DNA preservation in human remains.
    Vir: Forensic science international [Elektronski vir]. - ISSN 1872-6283 (Vol. 353, iss. [article no.] 111882, 2023, str. 1-8)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2023
    Jezik - angleški
    COBISS.SI-ID - 173613571

    Povezava(-e):

    https://www.sciencedirect.com/science/article/pii/S0379073823003328?via%3Dihub
    Repozitorij Univerze v Ljubljani – RUL
    DOI

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