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Gozdarski inštitut Slovenije, Gozdarska knjižnica, Ljubljana (GIS)
  • Microbial utilization of simple and complex carbon compounds in a temperate forest soil [Elektronski vir]
    Martinović, Tijana ...
    Forest soil processes carried out by microorganisms are critical for the global carbon (C) cycle and climate. Characterizing the utilization of differently recalcitrant C sources is an important step ... towards understanding the ecosystem-level function of microorganisms in temperate forest soils. Here, using stable-isotope probing (SIP), we tracked C incorporation into bacterial and fungal biomass by quantifying 13C incorporation into phospholipid fatty acids (PLFA-SIP), its respiration (i.e., content in the produced CO2) and C accumulation by individual microbial taxa (DNA-SIP), following the addition of 13C-labelled substrates of different recalcitrance (citric acid, glucose, chitin, cellulose, hemicellulose, and plant biomass) in microcosms. The highest 13C respiration was observed after the addition of the low-molecular-mass substrates citric acid and glucose, while the highest 13C incorporation into microbial biomass was observed during growth on chitin. Communities of fungi and bacteria that incorporated 13C of various origins into their biomass differed from the original soil communities, as well as between treatments. The most distinct microbial community was observed in microcosms containing 13C-chitin, indicating its utilization by both fungi and bacteria. Bacterial taxa were more often versatile, incorporating C of various origins, while there was a higher share of fungi that were specialists. Together, our results show that low-molecular-mass compounds that belong to typical root exudates are more readily respired, while the C from biopolymers studied was relatively more incorporated into microbial biomass. Various C sources are targeted by distinct microbial communities, although their composition partly overlaps due to the existence of generalist bacteria and fungi that are capable of utilizing various C sources.
    Vir: Soil biology & biochemistry. - ISSN 0038-0717 (Vol. 173, article no.108786, 2022, 10 str.)
    Vrsta gradiva - e-članek
    Leto - 2022
    Jezik - angleški
    COBISS.SI-ID - 145136899

    Povezava(-e):

    https://doi.org/10.1016/j.soilbio.2022.108786
    https://www.sciencedirect.com/science/article/pii/S0038071722002437
    DOI

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