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  • Effects of American foulbrood disease on honey bee (Apis mellifera) larvae = Delovanje hude gnilobe čebelje zalege na ličinko čebele (Apis mellifera)
    Gregorc, Aleš
    Histochemical and cytochemical techniques were used to study the characteristics of the midgut during the development of honeybee, Apis mellifera, larvae. Free acid phosphatase appears in the cell ... cytoplasm before morphological signs of cell death during normal larval devlopment. Immunohistochemical analyses were made after p.o. and p.c. inoculation with Paenibacillus larvae var. larvae, causative agent of American foulbrood disease in honeybee larvae where extensive induced or necrotic cell death was observed. Hsp 70 was localised in Paenibacillus infected midgut epithelial cells and was not found in healthy larval tissue. In non infected larvae exposed nuclear histone protein, confirmed programmed cell death during normaltissue turnover, but in Paenibacillus infected larvae DAB reaction product was found distributed in all cell nuclei and also in cell cytoplasm. In necrotically deleted midgut cells pyronin staining was absent and dense redpyronin staining was found in nuclei and in the cytoplasm of non infected cells. The use of histo- and immunohistochemical techniques and their applications in honeybee pathology are also discussed in this review.
    Vir: Slovenian veterinary research = Slovenski veterinarski zbornik : the scientific journal of the Veterinary Faculty University of Ljubljana. - ISSN 1580-4003 (Letn. 37, št. 4, 2000, str. 217-232)
    Vrsta gradiva - članek, sestavni del
    Leto - 2000
    Jezik - angleški
    COBISS.SI-ID - 1009018

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