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Onkološki inštitut Ljubljana (OILJ)
  • Uporabnost celic granuloze za normiranje citometričnih meritev : [magistrsko delo]
    Gazić, Barbara
    Background: The quantity of DNA in the nuclei of the cells measured by cytometry is expressed in arbitrary units and graphically presented by histogram. The DNA value of the sample is compared with ... DNA value of normal cells and expressed by the DNA index. The DNA index indicates a relative levelof deviation from normal diploid cells that have, by definition, a DNA index 1. The selection of appropriate reference cells is of crucial importancefor a proper determination of DNA ploidy. In the fluid of the ovarian follicle that has been aspirated for the purposes of IVF, there is an abundant quantity of granulosa cells that are usually rejected after the removal of oocyte. The aim of our study was to find out whether these granulosa cells (GC) could be applied in the standardization of the measurements of DNA ploidy in fresh, not fixated cells by image- and flow-cytometer. We therefore analyzed the cellular content of follicular fluid, its proliferation characteristics and DNA content of the GC. Methods: In the first series we collected all aspirated follicles of one woman into thesame sample (mixed follicles). We analyzed the follicular fluid sediments of 78 women with gonadotropin stimulated menstrual cycle, 10 women with natural
    Vrsta gradiva - magistrsko delo
    Založništvo in izdelava - Ljubljana : [B. Gazić], 2002
    Jezik - slovenski
    COBISS.SI-ID - 2398228

Signatura – lokacija, inventarna št. ... Status izvoda Rezervacija
Knjižnica
N MGR GAZIĆ B Uporabnost 0000000064
IN: 0012405
Knjižnica
N MGR GAZIĆ B Uporabnost 64
IN: 0012405
prosto - na dom, čas izposoje: 7 dni
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