Objective: To investigate whether Shen-Fu Injection(参附注射液, SFI) reduces post-resuscitation immune dysfunction in a porcine model of cardiac arrest by modulating apoptosis of regulatory T ...lymphocytes(Treg) in the spleen. Methods: After 8-min untreated ventricular fibrillation and 2-min basic life support, 24 pigs were divided into 3 groups with a random number table, i.e. SFI group, epinephrine(EP) group, and saline(SA) group(8 in each group), which received central venous injection of SFI(1.0 m L/kg), EP(0.02 mg/kg) and SA, respectively. The same procedure without CA initiation was achieved in the sham-operated(sham) group(n=6). After successful return of spontaneous circulation(ROSC), apoptosis rate of splenic Treg was detected by flow cytometry; and the m RNA expression of forkhead/winged helix transcription factor(Foxp3) of splenic Treg was detected by real time-polymerase chain reaction; and the levels of interleukin-4(IL-4) and interferon-γ(IFN-γ) in porcine splenic Treg were detected by using enzyme-linked immunosorbent assay(ELISA). Results: Compared with the sham group, the apoptosis rate of Treg was significantly decreased, and the levels of Foxp3 m RNA expression, IFN-γ, IL-4 and IFN-γ/IL-4 were increased in the SA group(P〈0.05 or P〈0.01). Compared with the EP and SA groups, SFI treatment increased the apoptosis rate of Treg and reduced the levels of Foxp3 m RNA expression, IFN-γ and IFN-γ/IL-4(P〈0.05). Conclusions: SFI has significant effects in attenuating post-resuscitation immune dysfunction by modulating apoptosis of Treg in the spleen.
Objective:To investigate the action of Shen-Fu Injection(参附注射液,SFI) in regulating the expression of the serum complements and inflammatory cytokines synthesized and released in response to the stress ...of global ischemia accompanying cardiac arrest(CA) and resuscitation.Methods:Thirty pigs were randomly divided into the sham(n=6) and 3 returns of spontaneous circulation(ROSC) groups(n=24).After 8-min untreated ventricular fibrillation and 2-min basic life support,24 pigs of the ROSC groups were randomized into three groups(n=8 per group),which received central venous injection of SFI(SFI group),epinephrine(EP group),or saline(SA group).Hemodynamic status and blood samples were obtained at 0,0.5,1,2,4,6,12,and 24 h after ROSC.Results:Serum concentrations of specific activation markers of the complement system C3,C4 and C5b-9 were increased during cardiopulmonary resuscitation th rough1 24 h after ROSC.There were intense changes of various pro-inflammatory cytokines and anti-inflammatory cytokines as early as 0.5 h after CA.Compared with the EP and SA groups,SFI treatment reduced the proinflammatory cytokines levels of interleukin(IL)-6,IL-8and tumor necrosis factor α(TNF-α,P〈0.05),and increased the anti-inflammatory cytokine levels of IL-4 and IL-10(P〈0.05).Further,SFI treatment decreased the values of C3,C4 and C5b-9 compared with the EP and SA groups.Conclusions:SFI,derived from the ancient Chinese medicine,has significant effects in attenuating post-resuscitation immune dysfunction by modulating the expression of complements and cytokines levels.The current study provided an experimental basis for the clinical application of a potential pharmacologic target for post resuscitation immune dysfunction.