The steroid hormone 20-hydroxyecdysone (20E) controls molting in arthropods. The timing of 20E production, and subsequent developmental transitions, is influenced by a variety of environmental ...factors including nutrition, photoperiod, and temperature, which is particularly relevant in the face of climate change. Environmental changes, combined with rapid urbanization, and the increasing prevalence of urban heat islands (UHI) have contributed to an overall decrease in biodiversity making it critical to understand how organisms respond to elevating global temperatures. Some arthropods, such as the Western black widow spider, Latrodectus hesperus, appear to thrive under UHI conditions, but the physiological mechanism underlying their success has not been explored. Here we examine the relationship between hemolymph 20E titers and spiderling development under non-urban desert (27°C), intermediate (30°C), and urban (33°C) temperatures. We found that a presumptive molt-inducing 20E peak observed in spiders at non-urban desert temperatures was reduced and delayed at higher temperatures. Intermolt 20E titers were also significantly altered in spiders reared under UHI temperatures. Despite the apparent success of black widows in urban environments, we noted that, coincident with the effects on 20E, there were numerous negative effects of elevated temperatures on spiderling development. The differential effects of temperature on pre-molt and intermolt 20E titers suggest distinct hormonal mechanisms underlying the physiological, developmental, and behavioral response to heat, allowing spiders to better cope with urban environments.
Deep neural networks have achieved impressive successes in fields ranging from object recognition to complex games such as Go
. Navigation, however, remains a substantial challenge for artificial ...agents, with deep neural networks trained by reinforcement learning
failing to rival the proficiency of mammalian spatial behaviour, which is underpinned by grid cells in the entorhinal cortex
. Grid cells are thought to provide a multi-scale periodic representation that functions as a metric for coding space
and is critical for integrating self-motion (path integration)
and planning direct trajectories to goals (vector-based navigation)
. Here we set out to leverage the computational functions of grid cells to develop a deep reinforcement learning agent with mammal-like navigational abilities. We first trained a recurrent network to perform path integration, leading to the emergence of representations resembling grid cells, as well as other entorhinal cell types
. We then showed that this representation provided an effective basis for an agent to locate goals in challenging, unfamiliar, and changeable environments-optimizing the primary objective of navigation through deep reinforcement learning. The performance of agents endowed with grid-like representations surpassed that of an expert human and comparison agents, with the metric quantities necessary for vector-based navigation derived from grid-like units within the network. Furthermore, grid-like representations enabled agents to conduct shortcut behaviours reminiscent of those performed by mammals. Our findings show that emergent grid-like representations furnish agents with a Euclidean spatial metric and associated vector operations, providing a foundation for proficient navigation. As such, our results support neuroscientific theories that see grid cells as critical for vector-based navigation
, demonstrating that the latter can be combined with path-based strategies to support navigation in challenging environments.
Urban fragmentation can reduce gene flow that isolates populations, reduces genetic diversity and increases population differentiation, all of which have negative conservation implications. ...Alternatively, gene flow may actually be increased among urban areas consistent with an urban facilitation model. In fact, urban adapter pests are able to thrive in the urban environment and may be experiencing human‐mediated transport. Here, we used social network theory with a population genetic approach to investigate the impact of urbanization on genetic connectivity in the Western black widow spider, as an urban pest model of human health concern. We collected genomewide single nucleotide polymorphism variation from mitochondrial and nuclear double‐digest RAD (ddRAD) sequence data sets from 210 individuals sampled from 11 urban and 10 nonurban locales across its distribution of the Western United States. From urban and nonurban contrasts of population, phylogenetic, and network analyses, urban locales have higher within‐population genetic diversity, lower between‐population genetic differentiation and higher estimates of genetic connectivity. Social network analyses show that urban locales not only have more connections, but can act as hubs that drive connectivity among nonurban locales, which show signatures of historical isolation. These results are consistent with an urban facilitation model of gene flow and demonstrate the importance of sampling multiple cities and markers to identify the role that urbanization has had on larger spatial scales. As the urban landscape continues to grow, this approach will help determine what factors influence the spread and adaptation of pests, like the venomous black widow spider, in building policies for human and biodiversity health.
Source‐to‐sink transfer of sediment and organic carbon (OC) is regulated by river mobility. Quantifying trends in river mobility is, however, challenging due to diverse planform morphologies (e.g., ...meandering, braided) and measurement methods. Here, we utilize a remote‐sensing method applicable to all planform morphologies to quantify the mobility timescales of 80 rivers worldwide. Results show that, across the continuum from meandering to braided rivers, there is a systematic reduction in the timescales of channel mobility and—to a lesser extent—floodplain reworking. This leads to a decrease in the efficiency with which braided rivers rework old floodplain material compared to their meandering counterparts. Reduced floodplain reworking efficiency of braided rivers leads to smaller channel‐belt areas relative to their size. Results suggest that river‐mobility timescales derived from remote sensing can aid in the characterization of sediment and OC storage and transit times at a global scale.
Plain Language Summary
Rivers transport sediment and organic carbon (OC) from the mountains to the sea, and river movement affects sediment and OC transit times in landscapes. Understanding the controls on timescales of river movement is critical for assessing how fluvial processes influence the terrestrial carbon cycle. While previous work has quantified different river planform shapes on the Earth's surface (e.g., meandering, wandering, and braided), little is known about how the pace and nature of river movement is different between river planform shapes. This is because we lack methods to measure and compare river movement across different river planform shapes. Here, we leverage a tool to quantify river movement from time series of satellite imagery that is equally applicable to all river planform shapes. We apply this method to calculate the timescales of river movement of 80 rivers worldwide for the last 37 years. We show that (a) braided rivers migrate faster than meandering rivers, and (b) braided rivers move over a smaller area relative to their size. Comparing results with geochemical observations from the Río Bermejo—a well‐studied river for sediment‐OC cycling—we show that our mobility framework may provide a new way to assess sediment and OC storage timescales in floodplains.
Key Points
We quantify timescales of floodplain reworking and channel‐overlap decay from satellite imagery of 80 rivers worldwide
From meandering to braided planform morphology, there is a reduction in the overlap timescale and the reworking timescale
Mobility timescales provide a means for remotely quantifying terrestrial‐sediment and organic‐carbon storage and transit
The presence of periodontal diseases (PDs) often strongly correlates with other severe chronic inflammatory conditions, including cardiovascular disease, diabetes, and arthritis. However, the ...mechanisms through which these diseases interact are unclear. In PD, tissue and bone destruction in the mouth is driven by elevated recruitment of polymorphonuclear neutrophils (PMNs), which are primed and recruited from the circulation to sites of inflammation. We predicted that systemic effects on PMN mobilization or priming could account for the interaction between PD and other inflammatory conditions. We tested this using a mouse model of ligature-induced PD and found elevated PMN counts specifically in bone marrow, supporting a systemic effect of periodontal tissue inflammation on PMN production. In contrast, mice with induced peritonitis had elevated PMN counts in the blood, peritoneum, and colon. These elevated counts were further significantly increased when acute peritonitis was induced after ligature-induced PD in mice, revealing a synergistic effect of multiple inflammatory events on PMN levels. Flow cytometric analysis of CD marker expression revealed enhanced priming of PMNs from mice with both PD and peritonitis compared to mice with peritonitis alone. Thus, systemic factors associated with PD produce hyperinflammatory PMN responses during a secondary infection. To analyze this systemic effect in humans, we induced gingival inflammation in volunteers and also found significantly increased activation of blood PMNs in response to ex vivo stimulation, which reverted to normal following resolution of gingivitis. Together, these results demonstrate that periodontal tissue inflammation has systemic effects that predispose toward an exacerbated innate immune response. This indicates that peripheral PMNs can respond synergistically to simultaneous and remote inflammatory triggers and therefore contribute to the interaction between PD and other inflammatory conditions. This suggests larger implications of PD beyond oral health and reveals potential new approaches for treating systemic inflammatory diseases that interact with PD.
While shifts in organismal biology stemming from climate change are receiving increased attention, we know relatively little about how organisms respond to other forms of anthropogenic disturbance. ...The urban heat island (UHI) effect describes the capture of heat by built structures (e.g. asphalt), resulting in elevated urban temperatures. The UHI is a well-studied phenomenon, but only a handful of studies have investigated trait-based shifts resulting from the UHI, and even fewer have attempted to quantify the magnitude of the UHI experienced at the microclimate scale. Here, using a common urban exploiter, the Western black widow spider (Latrodectus hesperus), we show that the UHI experienced by spiders in July in their urban Phoenix, AZ refuges is 6°C hotter (33°C) than conditions in the refuges of spiders from Sonoran Desert habitat outside of Phoenix's development (27°C). We then use this field microclimate UHI estimate to compare the development speed, mass gain and mortality of replicate siblings from 36 urban lineages reared at 'urban' and 'desert' temperatures. We show that extreme heat is slowing the growth of spiderlings and increasing mortality. In contrast, we show that development of male spiders to their penultimate moult is accelerated by 2 weeks. Lastly, in terms of behavioral shifts, UHI temperatures caused late-stage juvenile male spiders to heighten their foraging voracity and late-stage juvenile female spiders to curtail their web-building behavior. Trait-based approaches like the one presented herein help us better understand the mechanisms that lead to the explosive population growth of urban (sometimes invasive) species, possibly at the expense of urban biodiversity. Studies of organismal responses to the present day UHI can be used as informative surrogates that help us grasp the impact that projected climate change will have on biodiversity.
How autobiographical memories are represented in the human brain and whether this changes with time are questions central to memory neuroscience. Two regions in particular have been consistently ...implicated, the ventromedial prefrontal cortex (vmPFC) and the hippocampus, although their precise contributions are still contested. The key question in this debate, when reduced to its simplest form, concerns where information about specific autobiographical memories is located. Here, we availed ourselves of the opportunity afforded by multivoxel pattern analysis to provide an alternative to conventional neuropsychological and fMRI approaches, by detecting representations of individual autobiographical memories in patterns of fMRI activity. We examined whether information about specific recent (two weeks old) and remote (10 years old) autobiographical memories was represented in vmPFC and hippocampus, and other medial temporal and neocortical regions. vmPFC contained information about recent and remote autobiographical memories, although remote memories were more readily detected there, indicating that consolidation or a change of some kind had occurred. Information about both types of memory was also present in the hippocampus, suggesting it plays a role in the retrieval of vivid autobiographical memories regardless of remoteness. Interestingly, we also found that while recent and remote memories were both represented within anterior and posterior hippocampus, the latter nevertheless contained more information about remote memories. Thus, like vmPFC, the hippocampus too respected the distinction between recent and remote memories. Overall, these findings clarify and extend our view of vmPFC and hippocampus while also informing systems-level consolidation and providing clear targets for future studies.
Urbanization alters natural landscapes and creates unique challenges for urban wildlife. Similarly, the Urban Heat Island (UHI) effect can produce significantly elevated temperatures in urban areas, ...and we have a relatively poor understanding of how this will impact urban biodiversity. In particular, most studies quantify the UHI using broad-scale climate data rather than assessing microclimate temperatures actually experienced by organisms. In addition, studies often fail to address spatial and temporal complexities of the UHI. Here we examine the thermal microclimate and UHI experienced in the web of Western black widow spiders (Latrodectus hesperus), a medically-important, superabundant urban pest species found in cities across the Western region of North America. We do this using replicate urban and desert populations across an entire year to account for seasonal variation in the UHI, both within and between habitats. Our findings reveal a strong nighttime, but no daytime, UHI effect, with urban spider webs being 2–5 °C warmer than desert webs at night. This UHI effect is most prominent during the spring and least prominent in winter, suggesting that the UHI need not be most pronounced when temperatures are most elevated. Urban web temperatures varied among urban sites in the daytime, whereas desert web temperatures varied among desert sites in the nighttime. Finally, web temperature was significantly positively correlated with a spider's boldness, but showed no relationship with voracity towards prey, web size, or body condition. Understanding the complexities of each organism's thermal challenges, the “functional microclimate”, is crucial for predicting the impacts of urbanization and climate change on urban biodiversity and ecosystem functioning.
•Urban heat island (UHI) effects are often estimated from broad-scale temperature estimates, and finer scale studies of thermal microclimates experienced by urban biota are necessary.•We find a pronounced nighttime UHI in the webs of Western black widow spiders (Latrodectus hesperus).•We also found spatial complexity (thermal patchiness) for urban spiders during the day and desert spiders at night.•Lastly, we found hotter webs to house bolder spiders, but found no relation between web temperature and web size, body condition, or voracity towards prey.
We studied individual variation in the antipredator response of the fishing spider,
D.
triton, in a variety of developmental and behavioural contexts. We examined our data with respect to two general ...hypotheses: (1) startled spiders should modulate their boldness according to context-specific variables (e.g. developmental stage, feeding opportunities, mating opportunities, parental investment, body condition, etc.), and/or (2) an individual spider's boldness will be consistent (i.e. positively correlated) across contexts such that boldness in any given context is well predicted by boldness in other contexts. We found some support for the former, context-specific hypothesis and strong support for the latter, ‘behavioural-syndromes’ hypothesis. While spiders were significantly bolder in the presence versus absence of prey, boldness did not fluctuate with developmental stage, mating opportunities, parental investment or condition in the manner predicted. Instead, boldness in adult spiders was well predicted by a syndrome of positively correlated bold behaviours across functional contexts. We suggest that animal behaviour most often reflects a combination of context-specific behavioural optimization and context-general expression of behavioural syndromes, as was seen in this experiment. We further suggest that disentangling the relative magnitude of these two general mechanisms in a wide variety of taxa, covering many behavioural-ecological contexts, will provide great insights into (1) population ecology (e.g. the success of invasive/pest species and the failure of threatened species), (2) community ecology (e.g. species coexistence, trophic dynamics), and (3) evolutionary ecology (e.g. evolution of the multivariate behavioural phenotype).
Abstract
Developmental plasticity is known to influence the mean behavioral phenotype of a population. Yet, studies on how developmental plasticity shapes patterns of variation within populations are ...comparatively rare and often focus on a subset of developmental cues (e.g., nutrition). One potentially important but understudied developmental experience is social experience, as it is explicitly hypothesized to increase variation among individuals as a way to promote “social niches.” To test this, we exposed juvenile black widow spiders (Latrodectus hesperus) to the silk of conspecifics by transplanting them onto conspecific webs for 48 h once a week until adulthood. We also utilized an untouched control group as well as a disturbed group. This latter group was removed from their web at the same time points as the social treatment, but was immediately placed back on their own web. After repeatedly measuring adult behavior and web structure, we found that social rearing drove higher or significant levels of repeatability relative to the other treatments. Repeatability in the social treatment also decreased in some traits, paralleling the decreases observed in the disturbed treatments. Thus, repeated juvenile disturbance may decrease among-individual differences in adult spiders. Yet, social rearing appeared to override the effect of disturbance in some traits, suggesting a prioritization effect. The resulting individual differences were maintained over at least one-third of the adult lifespan and thus appear to represent stable, canalized developmental effects and not temporal state differences. These results provide proximate insight into how a broader range of developmental experiences shape trait variation.
Juvenile social experience increases adult behavioral differences, in some, but not all traits. This result indicates that juvenile experience may play an important role in shaping individual social niches. Unexpectedly, the disturbance from removing spiders from their containers generally reduced variation but increased how body size influenced behavior, suggesting that individuals have unique responses to different developmental experiences. These differences persisted for the duration of the experiment highlighting the long-term effects of developmental experience.
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