Abstract Introduction Flow cytometry allows the analysis of multiple antigens in a single tube at a single cell level. We present a rapid and sensitive two tube flow cytometric protocol for the ...detection of multiple platelet antigens and activation markers gated on a pure platelet population. Materials and methods The presence of platelet specific antigens was analyzed in citrated whole blood of normal platelets and from patients diagnosed with platelet abnormalities. Quiescent platelets as well as stimulated platelets were analyzed using a gating strategy based on ubiquitously expressed platelet membrane markers. A ubiquitously expressed platelet marker was combined with antibodies against the activated alpha2b-beta3 (PAC-1), Lysosomal Activated Membrane Protein (CD63) and P-selectin (CD62P). Results We were able to detect the platelet antigens CD36, CD41, CD42a, CD42b and CD61 in one single tube. Our approach allowed the single tube determination of PAC-1, CD63 and CD62P after activation of platelets by thrombin, collagen, ADP and PAR-1, and determination of platelet abnormalities. Conclusions Our two tube multi-parameter screening protocol is suited for the analysis of platelet antigens expressed on quiescent and activated platelets and allows the detection of aberrancies as found in blood of patients with thrombocytopathy such as Glanzmann Thrombasthenia, storage pool disease with diminished granule content and patients treated with clopidogrel and acetylsalicylic acid.
Abstract
Parts machined by high cutting speeds can often exhibit high fatigue strength, increased micro-hardness in the surface layers and plastic deformations, due to the tool cutting edge radius ...associated with the induced stresses. The changing of rake and clearance angles has an important influence on the chip formation, cutting forces, residual stresses, temperatures in both the workpiece and the tool. International research on the influence of geometric parameters of the tool on the entire cutting process, are of particular importance to understand this process development. The approach of this study, considers the parametric realization of the cutting tool profile - a coated TiC turning chisel, which will be used in the finite element simulation of the orthogonal turning process. Deform 2D application, which is a powerful simulation engine was chosen and allows the correct simulation of the cutting process in real machining conditions. Deform 2D enables the automatically meshing and remeshing generation and also the optimization whenever needed and wherever is required a high accuracy, thereby reducing the overall difficulty of the problem and the computational requirements. Using Lagrangian discretization, the machining process was simulated and made possible to observe and present a series of conclusions and own points of view regarding the temperature distribution at the tool tip and in the workpiece, the effective stresses distribution and the cutting force variation under the rake and clearance angles influences.
MicroRNAs (miRNAs) are small noncoding RNAs that play key roles in posttranscriptional gene regulation. Being involved in regulating virtually all cellular processes, from proliferation and ...differentiation to migration and apoptosis, they have emerged as important epigenetic players. While most interest has gone into which miRNAs are involved in specific cellular processes or pathologies, the dosage‐dependent effects of miRNAs remain vastly unexplored. Different doses of miRNAs can cause selective downregulation of target genes, in turn determining what signaling pathways and cellular responses are triggered. To explore this behavior, the effects of incremental miRNA dosage need to be studied; however, current delivery methods for miRNAs are unable to control how much miRNA enters a cell. Herein, an approach is presented based on a nanostraw–electroporation delivery platform that decouples the delivery from biological mechanisms (e.g., endocytosis) to enable precise control over the amount of miRNA delivered, along with demonstrating ratiometric intracellular delivery into primary dermal fibroblasts for miR‐181a and miR‐27a. In addition, it is shown that the nanostraw delivery platform allows efficient delivery of miRNAs into primary keratinocytes, opening new opportunities for successful miRNA delivery into this hard‐to‐transfect cell type.
MicroRNAs (miRNAs) are small noncoding RNAs that impact posttranscriptional gene regulation. Interestingly, different doses of miRNAs can selectively downregulate targeted genes. However, current delivery methods for miRNAs cannot control how much miRNA enters a cell. Herein, a nanostraw device enables miRNA dosage control, ratiometric intracellular delivery into primary human fibroblasts of multiple miRNAs, and efficient delivery into primary human keratinocytes.
This project showcases the effects of the homogenization treatments applied to 2724 cast aluminium alloy semi-products, as well as the results regarding the resistance and plasticity improvement of ...these semi-products. These results have proven the validity of the initial processing hypothesis, which states that certain modifications of the crystalline structure can lead to the improvement of the characteristics mentioned above. A highly resistant 2724 aluminium alloy sample, representing the Al-Cu-Mg system, was used for this experiment. At a structural level, the alloy is made of aluminium based solid α solution grains and a series of binary, ternary or complex intermetallic phases that can either be soluble or insoluble. This type of alloy is mostly used in the machine building or aeronautics industry. The results are according to the microstructural observations, the homogenization heat treatment effect leading to the removal of any chemical heterogeneity (dendritic segregation) and a balanced structure made of polyhedral crystals and a homogeneous dispersion throughout the whole mass of the alloy.
In the present study a new strategy of synthesis was developed for nanostructured calcium phosphates, such as hydroxyapatite (HAP) or silicon, magnesium and zinc modified hydroxyapatites of ...controlled characteristics (such as the shape and size of nanoparticles, porosity and crystallinity) by precipitation reactions. These inorganic powders were jointly used with chitosan (CHI) and collagen type I (COL) to manufacture fibrous biocomposite scaffolds made of self-assembled layers by using layer by layer technique for cell cultures. The resulting materials were characterized by using X-ray diffraction, SEM, TEM and AFM. This work also reports the structural data obtained on the surface of the newly prepared nanostructured porous scaffolds made of self-assembled nanoHAP–Si(0.2%)–Mg(0.6%)–Zn(0.2%)/CHI/COL layers at 7days in human osteoblastic cell culture. A new porous structure was found by SEM imaging on the surface of these scaffolds due to the cells and scaffold interactions. This structure is apparently similar to the bone tissue. Our data have indicated that the incorporation of small amounts of silicon, magnesium and zinc within the nanoHAP lattice improved the biological activity of human osteoblasts on these scaffolds, in vitro. The data have revealed the high importance of HAP nanostructure and its composition in the preparation of fibrous scaffolds with potential applications in particle technology, bone tissue engineering and nano medicine.
This work reports structure data obtained on the surface of newly prepared nanostructured porous scaffold, made of self-assembled nanoHAP–Si(0.2%)–Mg(0.6%)–Zn(0.2%)/chitosan/collagen layers, at 7days in human osteoblasts culture. A new porous structure rather similar to the new bone tissue was found on the scaffold surface revealing the high importance of the size and shape of HAP nanoparticles and collagen fibers for controlled biomaterial properties. Display omitted
► Nano hydroxyapatite contaning Si, Mg and Zn of controlled size distribution is obtained. ► Nano hydroxyapatite-Si-Mg-Zn/chitosan/collagen scaffolds are developed. ► Fibrous scaffolds show high performance of human osteoblasts in culture. ► New structure (probably bone like tissue) is developed by osteoblasts on fibrous scaffolds at 7days in cell culture.
Motivated by applications to proving regularity of solutions to degenerate parabolic equations arising in population genetics, we study existence, uniqueness, and the strong Markov property of weak ...solutions to a class of degenerate stochastic differential equations. The stochastic differential equations considered in our article admit solutions supported in the set 0,\infty )^n\times \mathbb{R}^m, and they are degenerate in the sense that the diffusion matrix is not strictly elliptic, as the smallest eigenvalue converges to zero at a rate proportional to the distance to the boundary of the domain, and the drift coefficients are allowed to have power-type singularities in a neighborhood of the boundary of the domain. Under suitable regularity assumptions on the coefficients, we establish existence of solutions that satisfy the strong Markov property, and uniqueness in law in the class of Markov processes.
Influenza is an important cause of morbidity and mortality in the elderly population each year. The often subtle clinical manifestations in the frail geriatric patients may not be recognized ...initially, impeding timely administration of antiviral treatment. The effectiveness of current influenza vaccines in the elderly population is often diminished by immune senescence. Increasing immunization rates among health-care workers and elderly caregivers, and finding more effective vaccines for the elderly people are likely to significantly improve disease prevention in this population at risk.
The aim of the paper is establishing a link between Big Data connected with Industry 4.0. We are developing a modern economy which is based on supply chains and logistics. Within each logistics are ...IT solutions. Some of the world's solutions are compiled in this paper and guidelines are set in the development of such solutions. Industry development 4.0, and in particular the development of Big data, gives us the possibility to introduce new ways of the industry. In the development of industry, maximizing production and reducing costs is extremely important. Real-time data and advanced algorithms give us the possibility to obtain real-time operational industry. Big data and their analysis are of great use in the industry because they improve the accuracy of the decision-making process. We must organize a structure that can design, manage and develop projects based on IoT and Big Data / Analytics technologies. In the industry, good sensor technology provides access to information. The paper has a focus on the managing the database with Big data in the Industry 4.0.
► Bi
2Sr
2Ca
2(Cu
1−
x
Zn
x
)
2O
6+d (
x
=
0; 0.02) thin films were prepared by DC magnetron sputtering. ► We examine the effects of oxygen doping on resistivity,
T
c, and transition width. ► In
x
=
...0.02 film, at doping level
p
=
0.1 anomalies were evidenced in Δ
T
c(
p) and
T
*(
p). ► From excess conductivity, change of
λ from −1/2 to −1/3 was evidenced around
p
=
0.1. ► The behavior near
p
=
0.1 is discussed by using STM results reported on Bi-2212 film.
The Bi
2Sr
2Ca(Cu
1−
x
Zn
x
)
2O
8+d (
x
=
0.00 and 0.02) thin films have been prepared in situ by DC magnetron sputtering and characterized by electrical resistivity and X-ray diffraction measurements. The oxygen content of the film was finely varied by thermal annealing. The evolution of the in-plane electrical resistivity versus temperature,
ρ(
T), in epitaxial
c-axis oriented thin films was investigated for states situated near optimal doped state (from light overdoped to underdoped states). The influence of doping on residual resistivity, critical transition temperature and transition width are presented. The
ρ(
T) curves of optimal and underdoped states were scaled with a universal function which describes the effect of pseudogap. The excess of conductivity for light underdoped samples was analyzed by using 1D, 2D and 3D Gaussian regimes and the genuine 3D-XY critical regime.
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