The use of proximity-dependent biotinylation approaches combined with mass spectrometry (e.g. BioID and APEX) has revolutionized the study of protein–protein interactions and organellar proteomics. ...These powerful techniques are based on the fusion of an enzyme (e.g. a biotin ligase or peroxidase) to a ‘bait’ protein of interest, which is then expressed in a relevant biological setting. Addition of enzyme substrate enables covalent biotin labeling of proteins in the vicinity of the bait in vivo. These approaches thus allow for the capture and identification of ‘neighborhood’ proteins in the context of a living cell, and provide data that are complementary to more established techniques such as fractionation or affinity purification. As compared to standard affinity-based purification approaches, proximity-dependent biotinylation (PDB) can help to: first, identify interactions with and amongst membrane proteins, and other polypeptide classes that are less amenable to study by standard pulldown techniques; second, enrich for transient and/or low affinity interactions that are not readily captured using affinity purification approaches; third, avoid post-lysis artefacts associated with standard biochemical purification experiments and; fourth, provide deep insight into the organization of membrane-less organelles and other subcellular structures that cannot be easily isolated or purified. Given the increasing use of these techniques to answer a variety of different types of biological questions, it is important to understand how best to design PDB–MS experiments, what type of data they generate, and how to analyze and interpret the results.
Many studies of visual processing are conducted in constrained conditions such as head- and gaze-fixation, and therefore less is known about how animals actively acquire visual information in natural ...contexts. To determine how mice target their gaze during natural behavior, we measured head and bilateral eye movements in mice performing prey capture, an ethological behavior that engages vision. We found that the majority of eye movements are compensatory for head movements, thereby serving to stabilize the visual scene. During movement, however, periods of stabilization are interspersed with non-compensatory saccades that abruptly shift gaze position. Notably, these saccades do not preferentially target the prey location. Rather, orienting movements are driven by the head, with the eyes following in coordination to sequentially stabilize and recenter the gaze. These findings relate eye movements in the mouse to other species, and provide a foundation for studying active vision during ethological behaviors in the mouse.
We investigate the effect of the population III (Pop III) stars supernova explosion (SN) on the high redshifts reionization history using the latest Planck data. It is predicted that massive Pop III ...stars (130 M⊙ ≤ M ≤ 270 M⊙) explode energetically at the end of their stellar life as pair-instability supernovae (PISNe). In the explosion, supernova remnants grow as hot ionized bubbles and enhance the ionization fraction in the early stage of the reionization history. This enhancement affects the optical depth of the cosmic microwave background (CMB) and generates the additional anisotropy of the CMB polarization on large scales. Therefore, analyzing the Planck polarization data allows us to examine the Pop III star SNe and the abundance of their progenitors, massive Pop III stars. In order to model the supernova(SN) contribution to reionization, we introduce a new parameter ζ, which relates to the abundance of the SNe to the collapse fraction of the Universe. Using the Markov chain Monte Carlo method with the latest Planck polarization data, we obtain the constraint on our model parameter, ζ. Our constraint tells us that observed CMB polarization is consistent with the abundance of PISNe predicted from the star formation rate and initial mass function of Pop III stars in recent cosmological simulations. We also suggest that combining further observations on the late reionization history, such as high redshift quasi-stellar object (QSO) observations, can provide tighter constraints and important information on the nature of Pop III stars.
A long-standing crucial question with atomic nuclei is whether or not α clustering occurs there. An α particle (helium-4 nucleus) comprises two protons and two neutrons, and may be the building block ...of some nuclei. This is a very beautiful and fascinating idea, and is indeed plausible because the α particle is particularly stable with a large binding energy. However, direct experimental evidence has never been provided. Here, we show whether and how α(-like) objects emerge in atomic nuclei, by means of state-of-the-art quantum many-body simulations formulated from first principles, utilizing supercomputers including K/Fugaku. The obtained physical quantities exhibit agreement with experimental data. The appearance and variation of the α clustering are shown by utilizing density profiles for the nuclei beryllium-8, -10 and carbon-12. With additional insight by statistical learning, an unexpected crossover picture is presented for the Hoyle state, a critical gateway to the birth of life.
This paper describes the design and performance of a 6-kW, full-bridge, bidirectional isolated dc-dc converter using a 20-kHz transformer for a 53.2-V, 2-kWh lithium-ion (Li-ion) battery energy ...storage system. The dc voltage at the high-voltage side is controlled from 305 to 355 V, as the battery voltage at the low-voltage side (LVS) varies from 50 to 59 V. The maximal efficiency of the dc-dc converter is measured to be 96.0% during battery charging, and 96.9% during battery discharging. Moreover, this paper analyzes the effect of unavoidable dc-bias currents on the magnetic-flux saturation of the transformer. Finally, it provides the dc-dc converter loss breakdown with more focus on the LVS converter.
The grain boundary diffusion process (GBDP) using a heavy rare earth elements (HRE) such as Dy and Tb is known as an effective method to enhance the coercivity of Nd–Fe–B sintered magnets without ...reducing remanence. This process has been industrially implemented to manufacture Nd–Fe–B based sintered magnets with high coercivity and high remanence. In this process, Dy is considered to diffuse through grain boundaries (GBs) to form (Nd1−xDyx)2Fe14B shells surrounding the Nd2Fe14B grains and the higher anisotropy field of the Dy-rich shell is considered to suppress the nucleation of reverse domains at low magnetic field. Although there are several investigations on the microstructure of HRE GBDP Nd–Fe–B magnets, no paper addressed the origin of the asymmetric formation of HRE rich shells. Based on detailed analysis of facet planes of core/shell interfaces, we propose a mechanism of the faceted core/shell microstructure formation in the GBDP sintered magnets. We believe that this gives new insights on understanding the coercivity enhancement by the GBDP. Display omitted
•Faceting was observed at the interfaces of cores and shells.•The core/shell interfaces are sharp with an abrupt change in Dy concentration.•Meting occurs at the interfaces of metalic Nd-rich/Nd2Fe14B phases above 685°C due to eutectic reaction.•Solidification of Dy-enriched liquid phase from 900°C can result in the shell formation.
Dysprosium enriched shell structure formed by the grain boundary diffusion process (GBDP) of a sintered Nd–Fe–B magnet was characterized by using scanning electron microscopy, electron back-scattered diffraction and transmission electron microscopy. Faceted core–shell interfaces with an abrupt change in Dy concentration suggest the Dy-rich shells are formed by the solidification of the liquid phase during cooling from the GBDP temperature. The Nd-rich phases are almost free from Dy, and their quantity near the surface of a bulk sample is much higher than that in the center, indicating that a higher fraction of a liquid phase exists near the surface during processing at 900°C. These microstructural features are explained on the basis of the phase equilibrium between Nd and Nd2Fe14B at the processing temperature and subsequent cooling. Based on the results, we discuss the coercivity enhancement by the GBDP using Dy vapor.
A successful example of high-Cr oxide dispersion strengthened (ODS) steels development is introduced with showing key technologies to overcome the issues to meet material requirements for next ...generation nuclear systems as well as fusion blanket systems. Corrosion issue requires Cr concentration more than 14wt.%, but aging embrittlement issue requires it less than 16wt.%. An addition of 4wt.%Al is effective to improve corrosion resistance of 16wt.%Cr-ODS steel in supercritical water (SCW) and lead–bismuth eutectics (LBE), while it is detrimental to high-temperature strength. An addition of small amount of Zr or Hf results in a significant increase in creep strength at 973K in Al-added ODS steels. Feasibility of high-Cr ODS steel without Al addition is assessed for fusion application in terms of corrosion resistance in SCW.
With nearly 1,100 species, the fish family Characidae represents more than half of the species of Characiformes, and is a key component of Neotropical freshwater ecosystems. The composition, ...phylogeny, and classification of Characidae is currently uncertain, despite significant efforts based on analysis of morphological and molecular data. No consensus about the monophyly of this group or its position within the order Characiformes has been reached, challenged by the fact that many key studies to date have non-overlapping taxonomic representation and focus only on subsets of this diversity.
In the present study we propose a new definition of the family Characidae and a hypothesis of relationships for the Characiformes based on phylogenetic analysis of DNA sequences of two mitochondrial and three nuclear genes (4,680 base pairs). The sequences were obtained from 211 samples representing 166 genera distributed among all 18 recognized families in the order Characiformes, all 14 recognized subfamilies in the Characidae, plus 56 of the genera so far considered incertae sedis in the Characidae. The phylogeny obtained is robust, with most lineages significantly supported by posterior probabilities in Bayesian analysis, and high bootstrap values from maximum likelihood and parsimony analyses.
A monophyletic assemblage strongly supported in all our phylogenetic analysis is herein defined as the Characidae and includes the characiform species lacking a supraorbital bone and with a derived position of the emergence of the hyoid artery from the anterior ceratohyal. To recognize this and several other monophyletic groups within characiforms we propose changes in the limits of several families to facilitate future studies in the Characiformes and particularly the Characidae. This work presents a new phylogenetic framework for a speciose and morphologically diverse group of freshwater fishes of significant ecological and evolutionary importance across the Neotropics and portions of Africa.