Epidemiological evidence emphasizes that ariboflavinosis can lead to oxidative stress, which in turn may mediate the initiation and progression of liver injury and intestinal inflammation. Although ...vitamin B
has gained worldwide attention for its antioxidant defense, the relationship between B
status, oxidative stress, inflammatory response, and intestinal homeostasis remains indistinct. Herein, we developed a B
depletion-repletion BALB/c mice model to investigate the ameliorative effects of B
bioenriched fermented soymilk (B
FS) on ariboflavinosis, accompanied by oxidative stress, inflammation, and gut microbiota modulation in response to B
deficiency.
results revealed that the phenotypic ariboflavinosis symptoms, growth rate, EGRAC status, and hepatic function reverted to normal after B
FS supplementation. B
FS significantly elevated CAT, SOD, T-AOC, and compromised MDA levels in the serum, simultaneously up-regulated Nrf2, CAT, and SOD2, and down-regulated Keap1 gene in the colon. The histopathological characteristics revealed significant alleviation in the liver and intestinal inflammation, confirmed by the downregulation of inflammatory (IL-1β and IL-6) and nuclear transcription (NF-κB) factors after B
FS supplementation. B
FS also increased the abundance and diversity of gut microbiota, increased the relative abundance of
and
, as well as decreased
,
,
, and
in strong conjunction with antioxidant, anti-inflammatory properties, and gut homeostasis along with the remarkable increase in cecal SCFAs content. We hereby reveal that B
FS can effectively alleviate deleterious ariboflavinosis associated with oxidative stress mediated liver injury, chronic intestinal inflammation, and gut dysbiosis in the B
depletion-repletion mice model
activation of the Nrf2 signaling pathway.
Endometrial cancer remains as one of the widespread female malignancies despite the existing treatment measures mainly surgery, radiotherapy, and chemotherapy. In recent times, studies have focused ...on medicinal plants such as ginger due to its multifaceted characteristics compared to conventional medicine. 6-Shogaol is regarded as the main active compound of ginger participating in pharmacological activities and combating various health disorders, especially cancer. In our study, we compared the effects of 6-gingerol, 6-paradol, and 6-shogaol on Ishikawa cells, and found 6-shogaol as a more effective ingredient against Ishikawa cell proliferation. Moreover, its promoted ferroptosis, as a result, triggered mitochondrial morphologic alternation, as well as changed iron concentration, GSH and MDA levels. Furthermore, 6-Shogaol inhibited cell metastasis by influencing cell invasion and migration. Finally, 6-shogaol could trigger PI3K/AKT signaling pathways in vitro and in vivo confirmed by western blotting assay and immunohistochemical evaluation. These findings suggest that 6-shogaol can be used as promising functional food component in health diet and in drug target methods for endometrial cancer therapy.
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•6-shogaol showed more toxicity in Ishikawa cells compared with 6-gingerol and 6-paradol.•6-shogaol promoted ferroptosis as well as changed iron concentration, GSH and MDA levels.•6-Shogaol inhibited cell metastasis by influencing cell invasion and migration.
•Extraction process of SF from broccoli seeds and sprouts was separately optimized.•SF yields decreased during germination process and varied among varieties.•Antioxidant capacity was significantly ...higher in sprouts than seeds.•3-Day-old broccoli sprouts were recommended as raw materials of functional foods.
The purpose of this study was to clearly understand the health-promoting potentials of broccoli seeds and sprouts according to identify their representative bioactive compounds and antioxidant activities in six varieties. Sulforaphane (SF) extraction was firstly optimized from seeds and sprouts. Then SF extracted under optimized conditions from seeds and sprouts were compared. Most varieties obtained the maximum SF, total phenolic (TP) and flavonoid (TF) contents in sprouts on day 3. SF contents in sprouts were 46%–97% of seeds, whereas TP and TF contents in sprouts were 1.12–3.58 times higher than seeds among varieties. After in vitro digestion, broccoli sprouts from MNL variety kept considerable SF, TF, and TP contents, as well as antioxidant capacities, with all values higher than seeds. Compared with seeds, sprouts after 3 days germination were also recommended as raw materials of functional foods that possess high health-promoting potential.
•The various biological and pharmacological properties of elderberry extracts were estimated.•Extracts of conventional dried elder fruits achieved the strongest antioxidant potential.•Ethanol extract ...of lyophilized fruits obtained by MAE extraction showed the greatest inhibitory action against tyrosinase and amylase enzymes.•Chlorogenic acid and rutin were the most abundant compounds in water and ethanol lyophilized extracts obtained by MAE and MAC extractions.•Sambucus nigra could be considered in the improvement of food and pharmaceuticals formulations.
The influence of drying and extraction processes on the phytochemical composition and biopotential of elderberry is challenging for the food industry. For this reason in this research two drying techniques (lyophilization and natural convection) and three extraction techniques (ultrasound (UAE), microwave (MAE), and conventional (maceration (MAC)) was applied using two”green” solvents (water and 50 % ethanol). Results of the research showed that lyophilization was a better way of drying than natural convection, while MAE extraction was the most efficient technique for the isolation of secondary metabolites. The most abundant phenolic compounds established by LC-MS/MS analysis, were chlorogenic acid and rutin, identified in extracts of lyophilized elderberries. Elderberry extracts achieved great antioxidant (CUPRAC: 2.30–5.13 mg TE/mL) and enzyme inhibitor potential (α-amylase: 0.51–8.34 mg ACAE/mL). The results suggest that elderberry is a rich natural source of bioactive compounds and could be used for the future development of dietary supplements and functional foods.
Maqui-berry (Aristotelia chilensis) is the emerging Chilean superfruit with high nutraceutical value. Until now, the research on this commodity was focused on the formulations enriched with ...polyphenols from the pulp. Herein, contents of tocols were compared in the seed oil of Maqui-berry obtained through three different extraction methods followed by determining their antioxidative and enzyme inhibitions in-vitro. Firstly, oilseed was extracted with n-hexane (Soxhlet method), chloroform/methanol/water (Bligh and Dyer method) and pressing (industrial). These samples were used to access their effects against DPPH, HORAC, ORAC, FRAP, Lipid-peroxidation (TBARS), α-amylase, α-glucosidase, and pancreatic lipase. All the isomers of tocopherol and tocotrienol were identified, and β-sitosterol was the only sterol found in higher amounts than other vegetable oils. The Bligh and Dyer method could lead to the highest antioxidative capacity compared to Soxhlet and press methods likely because the latter have a higher amount of tocopherols. Further, seed oil from Maqui berry and their tocols (α, β, γ, δ-tocopherols, tocotrienols, and β-sitosterol) warrant clinical investigation for their antioxidative and antiobesity potential. Taken together, these findings provide relevant and suitable conditions for the industrial processing of Maqui-berry.
•Phytochemicals from seed oils of A. chilensis showed antioxidant activities.•The IC50 concentration was between 1.8 and 39.8 μg/ml of samples.•These samples showed very good activities in ORAC, HORAC and FRAP measurements, respectively.•Tocopherols, tocotrienols and β-sitosterol were the most active metabolites.•The assayed compounds show inhibitory activity against digestive enzymes.
The Chilean plants
,
(Rhamnaceae),
(Monimiaceae), and
(Lauraceae) were evaluated against Codling moth:
L. (Lepidoptera: Tortricidae) and fruit fly
(Diptera: Drosophilidae), which is one of the most ...widespread and destructive primary pests of
(plums, cherries, peaches, nectarines, apricots, almonds), pear, walnuts, and chestnuts, among other. Four benzylisoquinoline alkaloids (coclaurine, laurolitsine, boldine, and pukateine) were isolated from the above mentioned plant species and evaluated regarding their insecticidal activity against the codling moth and fruit fly. The results showed that these alkaloids possess acute and chronic insecticidal effects. The most relevant effect was observed at 10 µg/mL against
and at 50 µg/mL against
, being the alteration of the feeding, deformations, failure in the displacement of the larvae in the feeding medium of
, and mortality visible effects. In addition, the docking results show that these type of alkaloids present a good interaction with octopamine and ecdysone receptor showing a possible action mechanism.
Metabolic syndrome (MetS) is a risk factor for the development of cardiovascular disease (CVD) and atherosclerosis through a mechanism that involves vascular smooth muscle cell (VSMC) proliferation, ...lipotoxicity and glucotoxicity. Several molecules found to be increased in MetS, including free fatty acids, fatty acid binding protein 4, leptin, resistin, oxidized lipoprotein particles, and advanced glycation end products, influence VSMC proliferation. Most of these molecules act through their receptors on VSMCs by activating several signaling pathways associated with ROS generation in various cellular compartments. ROS from NADPH-oxidase and mitochondria have been found to promote VSMC proliferation and cell cycle progression. In addition, most of the natural or synthetic substances described in this review, including pharmaceuticals with hypoglycemic and hypolipidemic properties, attenuate VSMC proliferation by their simultaneous modulation of cell signaling and their scavenging property due to the presence of a phenolic ring in their structure. This review discusses recent data in the literature on the role that several MetS-related molecules and ROS play in the change from contractile to proliferative phenotype of VSMCs. Hence the importance of proposing an appropriate strategy to prevent uncontrolled VSMC proliferation using antioxidants, hypoglycemic and hypolipidemic agents.
•VSMC proliferation in metabolic syndrome and obesity involves the generation of ROS.•Hyperlipidemia, hyperglycemia and ROS induce lipotoxicity and glucotoxicity in VSMC.•Adipokines influence VSMC proliferation through several signaling pathways.•Phytochemicals with antioxidant properties inhibit VSMC proliferation.
The present study emphasized on the anti-cancerous effects of dioscin and its underlying molecular mechanism in human endometrial cancer Ishikawa cells. Dioscin significantly suppressed the ...proliferation of Ishikawa cells at IC50 of 2.37 μM. Besides, dioscin could inhibit the proliferation of Ishikawa cells by blocking the G0/G1 cell cycle through up-regulation of p16, p21, and p27 and down-regulation of cycle-cellular protein (Cyclin A/D/E) and cyclin-dependent kinase (CDK2/4/6). Also, it promoted apoptosis through the mitochondrial pathway, including the regulation of Bcl family proteins, the increase of ROS levels, the activation of caspases (Caspase 9/3), and the decrease of mitochondrial membrane permeability. Whereas dioscin also effectively activated the marker genes and proteins (Fas, TNF-R1, and Caspase 8) related to the death receptor-mediated pathway which confirmed the involvement of both the pathways for dioscin-induced apoptosis. The current results demonstrated that dioscin possessed potential health benefits with respect to endometrial cancer prevention and treatment.
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•Dioscin inhibited Human Endometrial Carcinoma proliferation in Ishikawa cells.•Dioscin arrested Ishikawa cells at G1/G0 phase through the regulation of cycle-related signals.•Dioscin induced apoptosis by activating the mitochondrial and the death receptor pathways.
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