Jakarta Bay in Indonesia and its offshore island chain, the Thousand Islands, are facing extreme pollution. Surfactants and diesel-borne compounds from sewage and bilge water discharges are common ...pollutants. However, knowledge of their effects on reef fish physiology is scarce. This study investigated combined and single effects of a) the water accommodated fraction of diesel (WAF-D, determined by ƩEPA polycyclic aromatic hydrocarbons (PAHs)) and b) the surfactant linear alkylbenzene sulfonate (LAS) on metabolic performance of the coral reef fish Siganus guttatus. Responses to combinations of each pollutant with elevated temperature (+3°C) were determined. Short-term exposure to WAF-D led to a significant decrease in standard metabolic rates, while LAS led to an increase. During combined exposure, metabolic depression was observed. Effects of pollutants were not amplified by elevated temperature. This study highlights the need to reduce import of these pollutants and to avoid negative long-term effects on fish health.
•Surfactants and diesel-borne compounds were determined in Jakarta Bay and the Thousand Islands.•Exposure of Siganus guttatus to water accommodated fraction of diesel led to metabolic depression.•Exposure of Siganus guttatus to an anionic surfactant led to increased metabolic rates.•Combined exposure to both pollutants caused metabolic depression (interactive effect).•Effects of pollutants were not amplified by elevated temperature (no synergism).
Melanin is a widely distributed and striking dark-colored pigment produced by countless living organisms. Although a wide range of bioactivities have been recognized, there are still major ...constraints in using melanin for biotechnological applications such as its fragmentary known chemical structure and its insolubility in inorganic and organic solvents. In this study, a bacterial culture of
SV 21 produced two distinct forms of melanin: (1) a particulate, insoluble form as well as (2) a rarely observed water-soluble form. The here presented novel, acid-free purification protocol of purified particulate melanin (PPM) and purified dissolved melanin (PDM) represents the basis for an in-depth comparison of their physicochemical and biological properties, which were compared to the traditional acid-based precipitation of melanin (AM) and to a synthetic melanin standard (SM). Our data show that the differences in solubility between PDM and PPM in aqueous solutions may be a result of different adjoining cation species, since the soluble PDM polymer is largely composed of Mg
ions and the insoluble PPM is dominated by Ca
ions. Furthermore, AM shared most properties with SM, which is likely attributed to a similar, acid-based production protocol. The here presented gentler approach of purifying melanin facilitates a new perspective of an intact form of soluble and insoluble melanin that is less chemical altered and thus closer to its original biological form.