Among women, breast cancer (BC) is the leading cancer and the most common cause of cancer-related death between 30 and 69 years. Although lifestyle and diet are considered to have a role in global BC ...incidence pattern, the specific influence of dyslipidemia in BC onset and progression is not yet completely understood.
Fasting lipid profile (total cholesterol, LDL-C, HDL-C, and triglycerides) was prospectively assessed in 244 women with BC who were enrolled according to pre-set inclusion criteria: diagnosis of non-hereditary invasive ductal carcinoma; selection for surgery as first treatment, and no history of treatment with lipid-lowering or anti-diabetic drugs in the previous year. Pathological and clinical follow-up data were recorded for further inclusion in the statistical analysis.
Univariate associations show that BC patients with higher levels of LDL-C at diagnosis have tumors that are larger, with higher differentiation grade, higher proliferative rate (assessed by Ki67 immunostaining), are more frequently Her2-neu positive and are diagnosed in more advanced stages. Cox regression model for disease-free survival (DFS), adjusted to tumor T and N stages of TNM classification, and immunohistochemical subtypes, revealed that high LDL-C at diagnosis is associated with poor DFS. At 25 months of follow up, DFS is 12% higher in BC patients within the third LDL-C tertile compared to those in the first tertile.
This is a prospective study where LDL-C levels, at diagnosis, emerge as a prognostic factor; and this parameter can be useful in the identification and follow-up of high-risk groups. Our results further support a possible role for systemic cholesterol in BC progression and show that cholesterol metabolism may be an important therapeutic target in BC patients.
ABSTRACT
Local star-forming galaxies show properties that are thought to differ from galaxies in the early Universe. Among them, the ionizing stellar populations and the gas geometry make the recipes ...designed to derive chemical abundances from nebular emission lines to differ from those calibrated in the Local Universe. A sample of 1969 extreme emission-line galaxies (EELGs) at a redshift 0 ≲ z ≲ 0.49, selected from the Sloan Digital Sky Survey (SDSS) to be local analogues of high-redshift galaxies, was used to analyse their most prominent emission lines and to derive total oxygen abundances and nitrogen-to-oxygen ratios following the direct method in the ranges 7.7 < 12 + log(O/H) < 8.6 and –1.8 < log(N/O) < –0.8. They allow us to obtain new empirically calibrated strong-line methods and to evaluate other recipes based on photoionization models that can be later used for a chemical analysis of actively star-forming galaxies in very early stages of galaxy evolution. Our new relations are in agreement with others found for smaller samples of objects at higher redshifts. When compared with other relations calibrated in the local Universe, they differ when the employed strong-line ratio depends on the hardness of the ionizing radiation, such as O32 or Ne3O2, but they do not when the main dependence is on the ionization parameter, such as S23. In the case of strong-line ratios depending on N ii lines, the derivation of O/H becomes very uncertain due to the very high N/O values derived in this sample, above all in the low-metallicity regime. Finally, we adapt the Bayesian-like code H ii-chi-mistry for the conditions found in this kind of galaxies and we prove that it can be used to derive within errors both O/H and N/O, in consistency with the direct method.
Critically ill patients and patients with haematological cancer are HIV-negative populations at high risk of invasive fungal infections. In intensive-care units, candidaemia and intra-abdominal ...candidiasis predominate, but aspergillosis has emerged as a lethal, under-recognised cause of pneumonia. In patients with haematological malignancies or who have undergone stem-cell transplantations, pulmonary disease due to aspergillus and other mould diseases predominate. In this Series paper, we provide an update on risk assessment, new diagnostic strategies, and therapeutic approaches. New concepts have emerged for use of risk prediction rules and an evidence base now exists for inclusion of biomarkers (eg, galactomannan, 1,3-β-D-glucan, and PCR assays for Aspergillus spp) into early diagnostic and therapeutic strategies. Imaging techniques remain helpful for early diagnosis of pulmonary mould diseases, with PET techniques offering potential improvements in diagnostic specificity and evaluation of clinical response. Echinocandins and triazoles have been validated extensively for prophylaxis, empirical therapy, and targeted therapy, but an increase in intrinsically resistant fungi and emergence of secondary resistance as a result of drug-induced selection pressure are of major concern. Echinocandins remain a major component of treatment of invasive candidiasis and new triazoles are the best alternative for prophylaxis and therapy of invasive aspergillosis.
The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry ...with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what has been evaluated experimentally versus adopted based on existing data and experience. Multicentric evaluation demonstrated high levels of reproducibility based on strict implementation of the EuroFlow SOPs and antibody panels. Overall, the 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls in the EuroFlow centers have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database.
Considerable research has documented that exposure to traumatic events has negative effects on physical and mental health. Much less research has examined the predictors of traumatic event exposure. ...Increased understanding of risk factors for exposure to traumatic events could be of considerable value in targeting preventive interventions and anticipating service needs.
General population surveys in 24 countries with a combined sample of 68 894 adult respondents across six continents assessed exposure to 29 traumatic event types. Differences in prevalence were examined with cross-tabulations. Exploratory factor analysis was conducted to determine whether traumatic event types clustered into interpretable factors. Survival analysis was carried out to examine associations of sociodemographic characteristics and prior traumatic events with subsequent exposure.
Over 70% of respondents reported a traumatic event; 30.5% were exposed to four or more. Five types - witnessing death or serious injury, the unexpected death of a loved one, being mugged, being in a life-threatening automobile accident, and experiencing a life-threatening illness or injury - accounted for over half of all exposures. Exposure varied by country, sociodemographics and history of prior traumatic events. Being married was the most consistent protective factor. Exposure to interpersonal violence had the strongest associations with subsequent traumatic events.
Given the near ubiquity of exposure, limited resources may best be dedicated to those that are more likely to be further exposed such as victims of interpersonal violence. Identifying mechanisms that account for the associations of prior interpersonal violence with subsequent trauma is critical to develop interventions to prevent revictimization.
Major abdominal oncology surgery is associated with substantial postoperative loss of functional capacity, and exercise may be an effective intervention to improve outcomes. The aim of this study was ...to assess efficacy, feasibility and safety of a supervised postoperative exercise programme.
We performed a single-blind, parallel-arm, randomized trial in patients who underwent major abdominal oncology surgery in a tertiary university hospital. Patients were randomized to an early mobilization postoperative programme based on supervised aerobic exercise, resistance and flexibility training or to standard rehabilitation care. The primary outcome was inability to walk without human assistance at postoperative day 5 or hospital discharge.
A total of 108 patients were enrolled, 54 into the early mobilization programme group and 54 into the standard rehabilitation care group. The incidence of the primary outcome was nine (16.7%) and 21 (38.9%), respectively (P=0.01), with an absolute risk reduction of 22.2% 95% confidence interval (CI) 5.9–38.6 and a number needed to treat of 5 (95% CI 3–17). All patients in the intervention group were able to follow at least partially the exercise programme, although the performance among them was rather heterogeneous. There were no differences between groups regarding clinical outcomes or complications related to the exercises.
An early postoperative mobilization programme based on supervised exercises seems to be safe and feasible and improves functional capacity in patients undergoing major elective abdominal oncology surgery. However, its impact on clinical outcomes is still unclear.
NCT01693172.
ABSTRACT
We investigate the contribution of clumps and satellites to the galaxy mass assembly. We analysed spatially resolved HubbleSpace Telescope observations (imaging and slitless spectroscopy) of ...53 star-forming galaxies at z ∼ 1–3. We created continuum and emission line maps and pinpointed residual ‘blobs’ detected after subtracting the galaxy disc. Those were separated into compact (unresolved) and extended (resolved) components. Extended components have sizes ∼2 kpc and comparable stellar mass and age as the galaxy discs, whereas the compact components are 1.5 dex less massive and 0.4 dex younger than the discs. Furthermore, the extended blobs are typically found at larger distances from the galaxy barycentre than the compact ones. Prompted by these observations and by the comparison with simulations, we suggest that compact blobs are in situ formed clumps, whereas the extended ones are accreting satellites. Clumps and satellites enclose, respectively, ∼20 per cent and ≲80 per cent of the galaxy stellar mass, ∼30 per cent and ∼20 per cent of its star formation rate. Considering the compact blobs, we statistically estimated that massive clumps (M⋆ ≳ 109 M⊙) have lifetimes of ∼650 Myr, and the less massive ones (108 < M⋆ < 109 M⊙) of ∼145 Myr. This supports simulations predicting long-lived clumps (lifetime ≳ 100 Myr). Finally, ≲30 per cent (13 per cent) of our sample galaxies are undergoing single (multiple) merger(s), they have a projected separation ≲10 kpc, and the typical mass ratio of our satellites is 1:5 (but ranges between 1:10 and 1:1), in agreement with literature results for close pair galaxies.
The fundamental metallicity relation (FMR) states that galaxies of the same stellar mass but larger star formation rate (SFR) tend to have smaller gas-phase metallicity ( ). It is thought to be ...fundamental because it naturally arises from the stochastic feeding of star formation from external metal-poor gas accretion, a process extremely elusive to observe but essential according the cosmological simulations of galaxy formation. In this Letter, we show how the FMR emerges from the local anti-correlation between SFR surface density and Zg recently observed to exist in disk galaxies. We analytically derive the global FMR from the local law, and then show that both relations agree quantitatively when considering the star-forming galaxies of the Mapping Nearby Galaxies at APO (MaNGA) survey. Thus, understanding the FMR becomes equivalent to understanding the origin of the anti-correlation between SFR and metallicity followed by the set of star-forming regions of any typical galaxy. The correspondence between local and global laws is not specific to the FMR, so that a number of local relations should exist that are associated with known global relations.
We present a methodology for the definition of the matrices involved in stress based finite element approximations in three‐dimensional problems. In order to handle the increased complexity of the 3D ...case, a rotation of the global reference frame is used, which greatly simplifies the expressions involved. This rotation is also applicable to the 2D case. The main focus of this work concerns the matrices used in the implementation of the hybrid equilibrium approach. However, a similar complementary methodology (displacement based) is also presented, allowing for an efficient definition of the matrices required for dual analysis, wherein bounds of the solution error are obtained from pairs of complementary solutions, one compatible and the other equilibrated.
Most consensus leukemia & lymphoma antibody panels consist of lists of markers based on expert opinions, but they have not been validated. Here we present the validated EuroFlow 8-color antibody ...panels for immunophenotyping of hematological malignancies. The single-tube screening panels and multi-tube classification panels fit into the EuroFlow diagnostic algorithm with entries defined by clinical and laboratory parameters. The panels were constructed in 2-7 sequential design-evaluation-redesign rounds, using novel Infinicyt software tools for multivariate data analysis. Two groups of markers are combined in each 8-color tube: (i) backbone markers to identify distinct cell populations in a sample, and (ii) markers for characterization of specific cell populations. In multi-tube panels, the backbone markers were optimally placed at the same fluorochrome position in every tube, to provide identical multidimensional localization of the target cell population(s). The characterization markers were positioned according to the diagnostic utility of the combined markers. Each proposed antibody combination was tested against reference databases of normal and malignant cells from healthy subjects and WHO-based disease entities, respectively. The EuroFlow studies resulted in validated and flexible 8-color antibody panels for multidimensional identification and characterization of normal and aberrant cells, optimally suited for immunophenotypic screening and classification of hematological malignancies.
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