Abstract
In this study, the production of lactic acid from municipal forestry and greening wastes processed by steam explosion (SE) and enzymatic hydrolysis is reported. The liquid fraction ...hydrolysate (LFH) obtained after filtration of the steam‐exploded slurry (previously subjected to detoxification) and the enzymatic hydrolysate resulting from the water insoluble solid fraction (WIS‐derived hydrolysate, WDH) are studied as substrate for lactic acid fermentation using an integrated strategy. The fermentation process of WDH using
Bacillus coagulans
and
Lactobacillus rhamnosus
shows similar sugar conversion yields of about 0.9 g g
−1
(based on consumed glucose). In contrast, the use of detoxified LFH (d‐LFH) completely inhibits
B. coagulans
, making necessary a previous dilution with the WDH to trigger fermentation when using this microorganism. The best integrated strategy using both d‐LFH and WDH streams and
B. coagulans
as fermentative strain has a 1:3 d‐LFH:WDH ratio, yielding 0.93 g lactic acid g
−1
of consumed sugars and a lactic acid concentration of about 50 g L
−1
.
The concept of a window of opportunity in hidradenitis suppurativa (HS) management suggests that early initiation of biological therapy leads to better outcomes, though its timing remains ...uncertain.INTRODUCIONThe concept of a window of opportunity in hidradenitis suppurativa (HS) management suggests that early initiation of biological therapy leads to better outcomes, though its timing remains uncertain.We conducted a retrospective observational multicenter study, including consecutive patients with moderate to severe HS who initiated secukinumab treatment following prior failure with systemic antibiotics or adalimumab. Therapeutic burden was defined as the sum of previous systemic treatment cycles and previous major surgical interventions for HS. Patients were followed up for 24 weeks. Main outcomes were safety and effectiveness, assessed through the proportion of patients achieving HS Clinical Response (HiSCR) and a 55% reduction in International HS Severity Score System (IHS4-55). Additionally, potential predictors of response to secukinumab were studied. Analysis was performed on an intention-to-treat basis.METHODSWe conducted a retrospective observational multicenter study, including consecutive patients with moderate to severe HS who initiated secukinumab treatment following prior failure with systemic antibiotics or adalimumab. Therapeutic burden was defined as the sum of previous systemic treatment cycles and previous major surgical interventions for HS. Patients were followed up for 24 weeks. Main outcomes were safety and effectiveness, assessed through the proportion of patients achieving HS Clinical Response (HiSCR) and a 55% reduction in International HS Severity Score System (IHS4-55). Additionally, potential predictors of response to secukinumab were studied. Analysis was performed on an intention-to-treat basis.A total of 67 patients (33 men, 34 women) were included, with a mean age of 41.55 (11.94) years and a mean baseline IHS4 of 17.88 (11.13). The mean therapeutic burden was 6.06 (3.49). At week 24, 10.45% (7/67) of patients experienced adverse events, with three leading to treatment discontinuation. At week 24, 41.79% (28/67) of patients achieved HiSCR, and 44.78% (30/67) of patients achieved IHS4-55. HiSCR could not be calculated in 12 patients with a baseline AN count < 3. A lower therapeutic burden was significantly associated with a higher likelihood of achieving HiSCR and IHS4-55 at week 24.RESULTSA total of 67 patients (33 men, 34 women) were included, with a mean age of 41.55 (11.94) years and a mean baseline IHS4 of 17.88 (11.13). The mean therapeutic burden was 6.06 (3.49). At week 24, 10.45% (7/67) of patients experienced adverse events, with three leading to treatment discontinuation. At week 24, 41.79% (28/67) of patients achieved HiSCR, and 44.78% (30/67) of patients achieved IHS4-55. HiSCR could not be calculated in 12 patients with a baseline AN count < 3. A lower therapeutic burden was significantly associated with a higher likelihood of achieving HiSCR and IHS4-55 at week 24.Secukinumab showed safety and efficacy in real-world patients with HS, and the inverse correlation found between therapeutic burden and treatment response supports the concept of a window of opportunity, offering insights into its timing.CONCLUSIONSSecukinumab showed safety and efficacy in real-world patients with HS, and the inverse correlation found between therapeutic burden and treatment response supports the concept of a window of opportunity, offering insights into its timing.
Process of medical nutrition therapy Martín Folgueras, Tomás; Vidal Casariego, Alfonso; Álvarez Hernández, Julia ...
Nutrición hospitalaria : organo oficial de la Sociedad Española de Nutrición Parenteral y Enteral,
10/2022, Letnik:
39, Številka:
5
Journal Article
Odprti dostop
IntroductionMedical nutrition therapy is a very useful tool in maintaining and recovering the health of patients with disease-related malnutrition, although its implementation can be complex and is ...not without risks. Quality processes are understood as sets of activities that are related or interact to transform input elements into results. From the SENPE Management Work Group we present the process of medical nutrition therapy (PMNT), which aims to facilitate the management of clinical nutrition of a multidisciplinary nutrition support team in a hospital setting. This paper describes the seven sub-processes PMNT is comprised of, in addition to a previous nutritional screening sub-process. Each sub-process is divided into a first section with a technical sheet detailing its general aspects, while a second section proposes key objectives, quality indicators, and standards for their evaluation. .
Transcription factors (TFs) are key regulators of gene expression in all organisms. In eukaryotes, TFs are often represented by functionally redundant members of large gene families. Overexpression ...might prove a means to unveil the biological functions of redundant TFs; however, constitutive overexpression of TFs frequently causes severe developmental defects, preventing their functional characterization. Conditional overexpression strategies help to overcome this problem. Here, we report on the TRANSPLANTA collection of Arabidopsis lines, each expressing one of 949 TFs under the control of a β–estradiol‐inducible promoter. Thus far, 1636 independent homozygous lines, representing an average of 2.6 lines for every TF, have been produced for the inducible expression of 634 TFs. Along with a GUS‐GFP reporter, randomly selected TRANSPLANTA lines were tested and confirmed for conditional transgene expression upon β–estradiol treatment. As a proof of concept for the exploitation of this resource, β–estradiol‐induced proliferation of root hairs, dark‐induced senescence, anthocyanin accumulation and dwarfism were observed in lines conditionally expressing full‐length cDNAs encoding RHD6, WRKY22, MYB123/TT2 and MYB26, respectively, in agreement with previously reported phenotypes conferred by these TFs. Further screening performed with other TRANSPLANTA lines allowed the identification of TFs involved in different plant biological processes, illustrating that the collection is a powerful resource for the functional characterization of TFs. For instance, ANAC058 and a TINY/AP2 TF were identified as modulators of ABA‐mediated germination potential, and RAP2.10/DEAR4 was identified as a regulator of cell death in the hypocotyl–root transition zone. Seeds of TRANSPLANTA lines have been deposited at the Nottingham Arabidopsis Stock Centre for further distribution.
The thermal analysis of stratospheric balloon payloads is still a complex task due to the variable thermal environment during the flight phases as well as due to the convective effects during the ...ascent. An experiment named HERCCULES has been successfully launched from Esrange Space Centre (Kiruna, Sweden) in September 2023 as part of the REXUS/BEXUS programme. This experiment aimed of serving as a heat transfer and thermal environment characterization platform that allow to validate previously developed methodologies for the worst-case thermal environmental conditions’ selection and the thermal analysis of these platforms. The experiment has been developed in an academic environment and the design, manufacturing, integration and test activities were mainly performed by Bachelor, Master and Ph.D. students with the mentorship of the European Space Agency. A low-cost design was achieved by using Commercial Off-the-Shelf (COTS) without compromising its performance capabilities. The obtained measurements show a colder behavior of the external elements with regard to the worst-case analysis during the first part of the ascent phase. In addition, radiative fluxes measured during the flight show a deviation in the infrared downward flux of about -50Wm−2 at 5km of altitude and a maximum fluctuation of about the 20% in the solar fluxes measurements. Significant advancements have been made for similar missions in terms of mechanical standardization, the use of COTS sensors and electronic components, the improvement of the reliability of the thermal analysis and the development of a software design adhered to standards and recommendations for critical systems.
•A university experiment for a stratospheric balloon flight has been design using COTS.•The heat transfer and thermal environment characterization experiments on board HERCCULES are described.•An overview of the mechanical, electronic, thermal and software design and tests is included.•Preliminary results of the flight on board BEXUS 32 are presented.
Ablation of macroreentrant atrial tachycardia in patients with mechanical prosthetic valves represents a challenge for electrophysiologists, because of the complexity of the procedure and the ...potential complications. Moreover, the need for fluoroscopy in this type of procedure is greater, due to the risk of interference between the prosthetic valve and the ablation or mapping catheter. We present two cases of patients with mechanical prosthetic valves and atrial flutter who underwent successful ablation with no complications using the CartoUnivu™ tool, which integrates the electroanatomical map and the fluoroscopy image.
Our aim is to assess the changes in clinical nutrition and dietetics education of the residents in endocrinology and nutrition for the last 10 years.
During the yearly update course in Clinical ...Nutrition and Dietetics held by SEEN, a survey about medical training is conducted since 2006. Descriptive data for the last 10 years are reported.
Every hospital accredited for medical training in endocrinology and nutrition has a unit of nutrition, depending on endocrinology and nutrition departments in 94.5%. While 13.3% of the residents required a different hospital for training in nutrition in 2006, all of them had enough resources in their own hospital in 2018. The training started in their third year of residency in 62.6%. The rotation has been increasing its length and now it lasts 6 months for 66% of the residents. 41.9% of the residents think it should be longer than 6 months. Education in dietetics should be extended the most. The residents take part in study protocols or publications in 72.5%, against only 27.9% in 2009. There is still a percentage of 10.1% to 19% of the residents grading their training in nutrition as insufficient.
The residents consider their global training in nutrition has improved in these last 10 years, although there is still a chance for optimization in areas such as dietetics.
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