The application of DNA markers for the identification of biological samples from both human and non-human species is widespread and includes use in food authentication. In the food industry the ...financial incentive to substituting the true name of a food product with a higher value alternative is driving food fraud. This applies to British pork products where products derived from traditional pig breeds are of premium value. The objective of this study was to develop a genetic assay for regulatory authentication of traditional pig breed-labelled products in the porcine food industry in the United Kingdom.
The dataset comprised of a comprehensive coverage of breed types present in Britain: 460 individuals from 7 traditional breeds, 5 commercial purebreds, 1 imported European breed and 1 imported Asian breed were genotyped using the PorcineSNP60 beadchip. Following breed-informative SNP selection, assignment power was calculated for increasing SNP panel size. A 96-plex assay created using the most informative SNPs revealed remarkably high genetic differentiation between the British pig breeds, with an average FST of 0.54 and Bayesian clustering analysis also indicated that they were distinct homogenous populations. The posterior probability of assignment of any individual of a presumed origin actually originating from that breed given an alternative breed origin was > 99.5% in 174 out of 182 contrasts, at a test value of log(LR) > 0. Validation of the 96-plex assay using independent test samples of known origin was successful; a subsequent survey of market samples revealed a high level of breed label conformity.
The newly created 96-plex assay using selected markers from the PorcineSNP60 beadchip enables powerful assignment of samples to traditional breed origin and can effectively identify mislabelling, providing a highly effective tool for DNA analysis in food forensics.
The recently discovered transitional millisecond pulsar system J1023+0038 exposes a crucial evolutionary phase of recycled neutron stars for multiwavelength study. The system, comprising the neutron ...star itself, its stellar companion, and the surrounding medium, is visible across the electromagnetic spectrum from the radio to X-ray/gamma-ray regimes and offers insight into the recycling phase of millisecond pulsar evolution. Here, we report on multiple-epoch astrometric observations with the Very Long Baseline Array (VLBA) which give a system parallax of 0.731 + or - 0.022 milliarcseconds (mas) and a proper motion of 17.98 + or - 0.05 mas yr super(-1). By combining our results with previous optical observations, we are able to use the parallax distance of (ProQuest: Formulae and/or non-USASCII text omitted) pc to estimate the mass of the pulsar to be 1.71 + or - 0.16 M sub(middot in circle), and we are also able to measure the three-dimensional space velocity of the system to be 126 + or - 5 km s super(-1). Despite the precise nature of the VLBA measurements, the remaining ~3% distance uncertainty dominates the 0.16 M sub(middot in circle) error on our mass estimate.
Emissions inventories are crucial inputs to air quality simulations and represent a major source of uncertainty. Various methods have been adopted to optimise emissions inventories, yet in most cases ...the methods were only applied to total anthropogenic emissions. We have developed a new approach that updates a priori emission estimates by source sector, which are particularly relevant for policy interventions. At its core is a perturbed emissions ensemble (PEE), constructed by perturbing parameters in an a priori emissions inventory within their respective uncertainty ranges. This PEE is then input to an air quality model to generate an ensemble of forward simulations. By comparing the simulation outputs with observations from a dense network, the initial uncertainty ranges are constrained, and a posteriori emission estimates are derived. Using this approach, we were able to derive the transport sector NOx emissions for a study area centred around Beijing in 2016 based on a priori emission estimates for 2013. The absolute emissions were found to be 1.5–9 × 104 Mg, corresponding to a 57 %–93 % reduction from the 2013 levels, yet the night-time fraction of the emissions was 67 %–178 % higher. These results provide robust and independent evidence of the trends of traffic emission in the study area between 2013 and 2016 reported by previous studies. We also highlighted the impacts of the chemical mechanisms in the underlying model on the emission estimates derived, which is often neglected in emission optimisation studies. This work paves forward the route for rapid analysis and update of emissions inventories using air quality models and routine in situ observations, underscoring the utility of dense observational networks. It also highlights some gaps in the current distribution of monitoring sites in Beijing which result in an underrepresentation of large point sources of NOx.
ABSTRACT The transitional millisecond pulsar (MSP) binary system PSR J1023+0038 re-entered an accreting state in 2013 June in which it bears many similarities to low-mass X-ray binaries (LMXBs) in ...quiescence or near-quiescence. At a distance of just 1.37 kpc, PSR J1023+0038 offers an unsurpassed ability to study low-level accretion onto a highly magnetized compact object. We have monitored PSR J1023+0038 intensively using radio imaging with the Karl G. Jansky Very Large Array, the European VLBI Network and the Low Frequency Array, seeing rapidly variable, flat spectrum emission that persists over a period of six months. The flat spectrum and variability are indicative of synchrotron emission originating in an outflow from the system, most likely in the form of a compact, partially self-absorbed jet, as is seen in LMXBs at higher accretion rates. The radio brightness, however, greatly exceeds extrapolations made from observations of more vigorously accreting neutron star LMXB systems. We postulate that PSR J1023+0038 is undergoing radiatively inefficient "propeller-mode" accretion, with the jet carrying away a dominant fraction of the liberated accretion luminosity. We confirm that the enhanced γ-ray emission seen in PSR J1023+0038 since it re-entered an accreting state has been maintained; the increased γ-ray emission in this state can also potentially be associated with propeller-mode accretion. Similar accretion modes can be invoked to explain the radio and X-ray properties of the other two known transitional MSP systems XSS J12270-4859 and PSR J1824-2452I (M28I), suggesting that radiatively inefficient accretion may be a ubiquitous phenomenon among (at least one class of) neutron star binaries at low accretion rates.
The energetic, eclipsing millisecond pulsar J1816+4510 was recently discovered in a low-frequency radio survey with the Green Bank Telescope. With an orbital period of 8.7 hr and a minimum companion ...mass of 0.16 M sub(middot in circle), it appears to belong to an increasingly important class of pulsars that are ablating their low-mass companions. We report the discovery of the gamma -ray counterpart to this pulsar and present a likely optical/ultraviolet counterpart as well. Using the radio ephemeris, we detect pulsations in the unclassified gamma -ray source 2FGL J1816.5+4511, implying an efficiency of ~25% in converting the pulsar's spin-down luminosity into gamma -rays and adding PSR J1816+4510 to the large number of millisecond pulsars detected by Fermi. The likely optical/UV counterpart was identified through position coincidence (<0".1) and unusual colors. Assuming that it is the companion, with R = 18.27 + or - 0.03 mag and effective temperature > ~15,000 K, it would be among the brightest and hottest of low-mass pulsar companions and appears qualitatively different from other eclipsing pulsar systems. In particular, current data suggest that it is a factor of two larger than most white dwarfs of its mass but a factor of four smaller than its Roche lobe. We discuss possible reasons for its high temperature and odd size, and suggest that it recently underwent a violent episode of mass loss. Regardless of origin, its brightness and the relative unimportance of irradiation make it an ideal target for a mass, and hence a neutron star mass, determination.
The evolution of island populations in natural systems is driven by local adaptation and genetic drift. However, evolutionary pathways may be altered by humans in several ways. The wild boar (WB) ...(Sus scrofa) is an iconic game species occurring in several islands, where it has been strongly managed since prehistoric times. We examined genomic diversity at 49 803 single-nucleotide polymorphisms in 99 Sardinian WBs and compared them with 196 wild specimens from mainland Europe and 105 domestic pigs (DP; 11 breeds). High levels of genetic variation were observed in Sardinia (80.9% of the total number of polymorphisms), which can be only in part associated to recent genetic introgression. Both Principal Component Analysis and Bayesian clustering approach revealed that the Sardinian WB population is highly differentiated from the other European populations (FST=0.126-0.138), and from DP (FST=0.169). Such evidences were mostly unaffected by an uneven sample size, although clustering results in reference populations changed when the number of individuals was standardized. Runs of homozygosity (ROHs) pattern and distribution in Sardinian WB are consistent with a past expansion following a bottleneck (small ROHs) and recent population substructuring (highly homozygous individuals). The observed effect of a non-random selection of Sardinian individuals on diversity, FST and ROH estimates, stressed the importance of sampling design in the study of structured or introgressed populations. Our results support the heterogeneity and distinctiveness of the Sardinian population and prompt further investigations on its origins and conservation status.
The HIV drug resistance (HIVDR) prevention and assessment strategy, developed by the World Health Organization (WHO) in partnership with HIVResNet, includes monitoring of HIVDR early warning ...indicators, surveys to assess acquired and transmitted HIVDR, and development of an accredited HIVDR genotyping laboratory network to support survey implementation in resource-limited settings. As of June 2011, 52 countries had implemented at least 1 element of the strategy, and 27 laboratories had been accredited. As access to antiretrovirals expands under the WHO/Joint United Nations Programme on HIV/AIDS Treatment 2.0 initiative, it is essential to strengthen HIVDR surveillance efforts in the face of increasing concern about HIVDR emergence and transmission.
The first concerted multi-model intercomparison of halogenated very short-lived substances (VSLS) has been performed, within the framework of the ongoing Atmospheric Tracer Transport Model ...Intercomparison Project (TransCom). Eleven global models or model variants participated (nine chemical transport models and two chemistry–climate models) by simulating the major natural bromine VSLS, bromoform (CHBr3) and dibromomethane (CH2Br2), over a 20-year period (1993–2012). Except for three model simulations, all others were driven offline by (or nudged to) reanalysed meteorology. The overarching goal of TransCom-VSLS was to provide a reconciled model estimate of the stratospheric source gas injection (SGI) of bromine from these gases, to constrain the current measurement-derived range, and to investigate inter-model differences due to emissions and transport processes. Models ran with standardised idealised chemistry, to isolate differences due to transport, and we investigated the sensitivity of results to a range of VSLS emission inventories. Models were tested in their ability to reproduce the observed seasonal and spatial distribution of VSLS at the surface, using measurements from NOAA's long-term global monitoring network, and in the tropical troposphere, using recent aircraft measurements – including high-altitude observations from the NASA Global Hawk platform. The models generally capture the observed seasonal cycle of surface CHBr3 and CH2Br2 well, with a strong model–measurement correlation (r ≥ 0.7) at most sites. In a given model, the absolute model–measurement agreement at the surface is highly sensitive to the choice of emissions. Large inter-model differences are apparent when using the same emission inventory, highlighting the challenges faced in evaluating such inventories at the global scale. Across the ensemble, most consistency is found within the tropics where most of the models (8 out of 11) achieve best agreement to surface CHBr3 observations using the lowest of the three CHBr3 emission inventories tested (similarly, 8 out of 11 models for CH2Br2). In general, the models reproduce observations of CHBr3 and CH2Br2 obtained in the tropical tropopause layer (TTL) at various locations throughout the Pacific well. Zonal variability in VSLS loading in the TTL is generally consistent among models, with CHBr3 (and to a lesser extent CH2Br2) most elevated over the tropical western Pacific during boreal winter. The models also indicate the Asian monsoon during boreal summer to be an important pathway for VSLS reaching the stratosphere, though the strength of this signal varies considerably among models. We derive an ensemble climatological mean estimate of the stratospheric bromine SGI from CHBr3 and CH2Br2 of 2.0 (1.2–2.5) ppt, ∼ 57 % larger than the best estimate from the most recent World Meteorological Organization (WMO) Ozone Assessment Report. We find no evidence for a long-term, transport-driven trend in the stratospheric SGI of bromine over the simulation period. The transport-driven interannual variability in the annual mean bromine SGI is of the order of ±5 %, with SGI exhibiting a strong positive correlation with the El Niño–Southern Oscillation (ENSO) in the eastern Pacific. Overall, our results do not show systematic differences between models specific to the choice of reanalysis meteorology, rather clear differences are seen related to differences in the implementation of transport processes in the models.
This study examined whether Heritage Language Learners (HLLs) of English display profile effects in their performance on knowledge- and processing-dependent measures relative to the standardised mean ...scores of monolingual speakers. The study also investigated the influence of several experiential factors on HLL performance.
Participants were 59 Arabic-speaking HLLs from six to nine years old. The children completed a battery of linguistic tests in their L1 and L2, as well as cognitive measures of short-term and working memory and non-verbal intelligence.
Significantly lower standardised scores were observed for HLLs as compared to the standardised mean scores on all Arabic/English language tasks except L2 word reading. HLLs scored at or above age-level expectations on cognitive measures except the Arabic nonword repetition task. Stepwise regression analyses examining variance in HLLs' performance, age and richness of environment consistently explained HLLs' performance in L1 Arabic, but different factors accounted for HLLs' performance in English depending on the task. Age was the only variable that consistently explained variance in performance on the cognitive measures.
The results suggest that processing-dependent measures may be less sensitive to difference in language experience than traditional knowledge-based measures such as standardised measures of language and vocabulary.
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