Abstract Renal transplantation is the best therapeutic option for patients with end-stage renal disease. However, long-term results have not been very encouraging because of patient deaths due to ...cardiovascular disease and chronic transplant nephropathy (CTN), which includes vascular damage similar to arteriosclerosis injury. Several autoantibodies have been related to vasculopathy in the transplant such as anti-β2GPI IgA, IgG, and IgM autoantibodies. We analyzed the levels of these autoantibodies among a cohort of 176 transplant recipients and a control group of 80 healthy subjects using enzyme-linked immunosorbent assay (ELISA). We collected data concerning the cardiovascular status of the patients, such as age, sex, diabetes mellitus, biopsy-confirmed CTN, schemic cardiopathy, cholesterol, triglycerides, and renal status by Modification of Diet in Renal Disease (MDRD) clearance and proteinuria. We also selected other characteristics, including hepatitis C virus infection and systolic/diastolic arterial pressures. The proportion of patients with high levels of IgG and IgM anti-β2GPI autoantibodies did not differ from that observed in the control group, whereas the difference became significant in the case of anti-β2GPI IgA autoantibodies (19.88% vs 1%). These results for the presence of anti-β2GPI IgA autoantibodies were related to clinical data through a multivariate analysis, where the only parameter influenced by the presence of these autoantibodies seemed to be proteinuria, which in most cases was due to CTN.
Gamma interferon (IFN-gamma) and the cellular responses induced by it are essential for controlling mycobacterial infections. Most patients bearing an IFN-gamma receptor ligand-binding chain ...(IFN-gammaR1) deficiency present gross mutations that truncate the protein and prevent its expression, giving rise to severe mycobacterial infections and, frequently, a fatal outcome. In this report a new mutation that affects the IFN-gammaR1 ligand-binding domain in a Spanish patient with mycobacterial disseminated infection and multifocal osteomyelitis is characterized. The mutation generates an amino acid change that does not abrogate protein expression on the cellular surface but that severely impairs responses after the binding of IFN-gamma (CD64 and HLA class II induction and tumor necrosis factor alpha and interleukin-12 production). A patient's younger brother, who was also probably homozygous for the mutation, died from meningitis due to Mycobacterium bovis. These findings suggest that a point mutation may be fatal when it affects functionally important domains of the receptor and that the severity is not directly related to a lack of IFN-gamma receptor expression. Future research on these nontruncating mutations will make it possible to develop new therapeutical alternatives in this group of patients.
The scientific performance of the Planck Low Frequency Instrument (LFI) after one year of in-orbit operation is presented. We describe the main optical parameters and discuss photometric calibration, ...white noise sensitivity, and noise properties. A preliminary evaluation of the impact of the main systematic effects is presented. For each of the performance parameters, we outline the methods used to obtain them from the flight data and provide a comparison with pre-launch ground assessments, which are essentially confirmed in flight.
Abstract The limited availability of organs for liver transplantation has focused interest on the use of cell transplants to restore hepatic function. Advances have been made in rodent models, but ...efficacy is limited in humans due to low engraftment efficiency. In rodents, pretransplantation treatment of the liver with engraftment enhancers (EE) shows that repopulation is feasible, although the toxicity of the substances impedes their application in humans. Evaluation of low-toxicity engraftment enhancers for human use requires testing in animal models, a time-consuming, expensive process that also raises ethical issues. To reduce animal use in the preliminary evaluation of a new EE, we designed an easily quantitated in vitro method that mimics an intraportal cell transplant. It is based on EE-mediated disruption of intercellular adhesion in confluent endothelial cell cultures.
In this paper we present the Low Frequency Instrument (LFI), designed and developed as part of the Planck space mission, the ESA programme dedicated to precision imaging of the cosmic microwave ...background (CMB). Planck-LFI will observe the full sky in intensity and polarisation in three frequency bands centred at 30, 44 and 70 GHz, while higher frequencies (100–850 GHz) will be covered by the HFI instrument. The LFI is an array of microwave radiometers based on state-of-the-art indium phosphide cryogenic HEMT amplifiers implemented in a differential system using blackbody loads as reference signals. The front end is cooled to 20 K for optimal sensitivity and the reference loads are cooled to 4 K to minimise low-frequency noise. We provide an overview of the LFI, discuss the leading scientific requirements, and describe the design solutions adopted for the various hardware subsystems. The main drivers of the radiometric, optical, and thermal design are discussed, including the stringent requirements on sensitivity, stability, and rejection of systematic effects. Further details on the key instrument units and the results of ground calibration are provided in a set of companion papers.
The present paper studies the structure of characteristic varieties of fundamental groups of graph manifolds. As a consequence, a simple proof of Papadima's question is provided on the ...characterization of algebraic links that have quasi-projective fundamental groups. The type of quasi-projective obstructions used here are in the spirit of Papadima's original work.
PDF
