Magnetic resonance microscopy (MRM), when used in conjunction with active staining, can produce high-resolution, high-contrast images of the mouse brain. Using MRM, we imaged in situ the fixed, ...actively stained brains of C57BL/6J mice in order to characterize the neuroanatomical phenotype and produce a digital atlas. The brains were scanned within the cranium vault to preserve the brain morphology, avoid distortions, and to allow an unbiased shape analysis. The high-resolution imaging used a T1-weighted scan at 21.5 μm isotropic resolution, and an eight-echo multiecho scan, post-processed to obtain an enhanced T2 image at 43 μm resolution. The two image sets were used to segment the brain into 33 anatomical structures. Volume, area, and shape characteristics were extracted for all segmented brain structures. We also analyzed the variability of volumes, areas, and shape characteristics. The coefficient of variation of volume had an average value of 7.0%. Average anatomical images of the brain for both the T1-weighted and T2 images were generated, together with an average shape atlas, and a probabilistic atlas for 33 major structures. These atlases, with their associated metadata, will serve as baseline for identifying neuroanatomical phenotypes of additional strains, and mouse models now under study. Our efforts were directed toward creating a baseline for comparison with other mouse strains and models of neurodegenerative diseases.
This article analyses the simulation of evaporative VOCs losses from storage tanks of gasoline and its anti-knock additives using TANKS 409d software. The VOCs emissions were estimated for different ...types of storage tanks: internal floating roof tank (IFRT), external floating roof tank (EFRT) from the one tank farmer of Romanian oil company. The obtained results show quantities of evaporated losses of fugitive VOCs emission approximately 165 t annually, between 01 August 2021 – 31 July 2022. The hazard evaluation of some components (toluene and anti-knock additives) from commercial gasoline has done by the computerize tool ALOHA in different scenarios during the operation of storage tanks. Using MARPOL application software, the pollutant threat zone has been mapped from ALOHA Gaussian model application.
Determination of acetaminophen and its main impurities: 4-nitrophenol, 4'-chloroacetanilide, as well as 4-aminophenol and its degradation products, p-benzoquinone and hydroquinone has been developed ...and validated by a new high-performance liquid chromatography method. Chromatographic separation has been obtained on a Hypersil Duet C18/SCX column, using gradient elution, with a mixture of phosphate buffer (pH = 4.88) and methanol as a mobile phase. Analysis time did not exceed 14.5 min and good resolutions, peak shapes and asymmetries have resulted. The linearity of the method has been tested in the range of 5.0-60 µg/mL for acetaminophen and 0.5-6 µg/mL for the other compounds. The limits of detection and quantification have been also established to be lower than 0.1 µg/mL and 0.5 µg/mL, respectively. The method has been successfully applied for the analysis of commercial acetaminophen preparations.
Almost worldwide, urban expansion has been increasing at a rapid pace in the last decades. Although metropolitan areas strongly contribute to economic growth, they are also usually characterized by ...uncoordinated urban development that consequently causes overburdened infrastructure and higher air pollution. Bucharest (the capital of Romania) and the surrounding Ilfov County underwent a significant expansion during different growth stages, roughly tripling their population since 1930.The present study analyzes the evolution of the urban spatial expansion of Bucharest and Ilfov between 1985 and 2015, using the World Settlement Footprint Evolution dataset created by the German Aerospace Center. The research integrates data provided by the National Institute of Statistics in relation to the resident population, population density, the average monthly net income and the gross domestic product. The results reveal that Bucharest - Ilfov development region had an impressive expansion during the investigated time interval in comparison with the other Romanian regions. In 30 years, the built-up area corresponding to Bucharest and the Ilfov County doubled, 2007 being the most prolific for the development of new built-up areas. In what concerns Bucharest city, the most noteworthy urban expansion occurred in District 1. Several urban growth hotspots were also identified and analyzed in detail (e.g., North of the capital).
The KASCADE-Grande experiment Apel, W.D.; Arteaga, J.C.; Badea, A.F. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
08/2010, Letnik:
620, Številka:
2
Journal Article
Recenzirano
Odprti dostop
KASCADE-Grande is the enlargement of the KASCADE extensive air shower detector, realized to expand the cosmic ray studies from the previous 10
14–10
17
eV primary energy range to 10
18
eV. This is ...performed by extending the area covered by the KASCADE electromagnetic array from 200×200 to 700×700
m
2 by means of 37 scintillator detector stations of 10
m
2 area each. This new array is named Grande and provides measurements of the all-charged particle component of extensive air showers (
N
ch
), while the original KASCADE array particularly provides information on the muon content
(
N
μ
)
. Additional dense compact detector set-ups being sensitive to energetic hadrons and muons are used for data consistency checks and calibration purposes. The performance of the Grande array and its integration into the entire experimental complex is discussed. It is demonstrated that the overall observable resolutions are adequate to meet the physical requirements of the measurements, i.e. primary energy spectrum and elemental composition studies in the primary cosmic ray energy range of 10
16–10
18
eV.
Small-animal imaging is an essential tool that provides noninvasive, longitudinal insight into novel cancer therapies. However, considerable variability in image analysis techniques can lead to ...inconsistent results. We have developed quantitative imaging for application in the preclinical arm of a coclinical trial by using a genetically engineered mouse model of soft tissue sarcoma. Magnetic resonance imaging (MRI) images were acquired 1 day before and 1 week after radiation therapy. After the second MRI, the primary tumor was surgically removed by amputating the tumor-bearing hind limb, and mice were followed for up to 6 months. An automatic analysis pipeline was used for multicontrast MRI data using a convolutional neural network for tumor segmentation followed by radiomics analysis. We then calculated radiomics features for the tumor, the peritumoral area, and the 2 combined. The first radiomics analysis focused on features most indicative of radiation therapy effects; the second radiomics analysis looked for features that might predict primary tumor recurrence. The segmentation results indicated that Dice scores were similar when using multicontrast versus single T2-weighted data (0.863 vs 0.861). One week post RT, larger tumor volumes were measured, and radiomics analysis showed greater heterogeneity. In the tumor and peritumoral area, radiomics features were predictive of primary tumor recurrence (AUC: 0.79). We have created an image processing pipeline for high-throughput, reduced-bias segmentation of multiparametric tumor MRI data and radiomics analysis, to better our understanding of preclinical imaging and the insights it provides when studying new cancer therapies.
A composition analysis of KASCADE air shower data is performed by means of unfolding the two-dimensional frequency spectrum of electron and muon numbers. Aim of the analysis is the determination of ...energy spectra for elemental groups representing the chemical composition of primary cosmic rays. Since such an analysis depends crucially on simulations of air showers the two different hadronic interaction models QGSJet and SIBYLL are used for their generation. The resulting primary energy spectra show that the knee in the all particle spectrum is due to a steepening of the spectra of light elements but, also, that neither of the two simulation sets is able to describe the measured data consistently over the whole energy range with discrepancies appearing in different energy regions.
Following the fall of communism in 1989, the R&D sector started a modernization process. By establishing in 1995 a structure dedicated to the space activities – Romanian Space Agency (ROSA) – the ...Government recognized the importance of this innovative strategic domain. The National Space Strategy defined by means of the RTD policy, with effects on both academia and industry, had three objectives since the 90’s: Participation to international space missions and programmes, Development of specific national projects and Capacity building at national level. In time, the experience acquired in the implementation of CIS, LCCS and LPIS/IACS projects offered the possibility to establish new certitudes for the evolution of the domain. The main development directions had been oriented towards the implementation of dedicated applications to answer problematics concerning smart agriculture, traceability, better agro-environmental practices and the relation with other scientific domains (e.g. cultural heritage). The partnership between a strong Governmental R&D structure – ROSA – and the University of Agronomic Sciences and Veterinary Medicine of Bucharest (USAMVB) demonstrates the capacity to find solutions for promoting space EO technologies and adapt the higher education curricula in synergy with the development of the ambitious European space programme evolution.
Cultural heritage represents the link between past and present civilizations, contributing to the right appreciation of the present-day by understanding the historical evolution of different human ...communities that are shaping our world. Preservation of the cultural heritage and mitigating potential risks on invaluable artifacts or objectives is key to conserving the identity of present and future generations. Our complex project is dedicated to monitoring, conservation, protection and promotion of the Romanian cultural heritage. It addresses the challenge of better safeguarding the cultural heritage by developing a multidisciplinary approach.The combined use of Synthetic Aperture Radar and multispectral satellite imagery represent a non-invasive investigation instrument that enable multitemporal analysis, contributing with valuable insights regarding cultural heritage sites. Remote sensing techniques were used to generate geospatial products such as displacement maps, urban sprawl evolution maps, urban heat island maps and many more.For protecting important artifacts, innovative recipes based on nanotechnologies were developed. In order to promote these artifacts, 3D photorealistic models were created using 3D reconstruction technology. For selected cultural heritage objectives, an integrated LiDAR aerial mapping system has enabled the creation of a high accuracy 3D model.This paper illustrates how different technologies, ranging from remote sensing to nanotechnology, could be used for safeguarding and promoting the Romanian cultural heritage and represents a first step in establishing and consolidating a multidisciplinary team involved in this domain.
The nature of ultrahigh-energy cosmic rays (UHECRs) at energies >1020 eV remains a mystery. They are likely to be of extragalactic origin, but should be absorbed within ∼50 Mpc through interactions ...with the cosmic microwave background. As there are no sufficiently powerful accelerators within this distance from the Galaxy, explanations for UHECRs range from unusual astrophysical sources to exotic string physics. Also unclear is whether UHECRs consist of protons, heavy nuclei, neutrinos or γ-rays. To resolve these questions, larger detectors with higher duty cycles and which combine multiple detection techniques are needed. Radio emission from UHECRs, on the other hand, is unaffected by attenuation, has a high duty cycle, gives calorimetric measurements and provides high directional accuracy. Here we report the detection of radio flashes from cosmic-ray air showers using low-cost digital radio receivers. We show that the radiation can be understood in terms of the geosynchrotron effect. Our results show that it should be possible to determine the nature and composition of UHECRs with combined radio and particle detectors, and to detect the ultrahigh-energy neutrinos expected from flavour mixing.
PDF
