HrpZ, a type three secretion system helper protein from the plant-pathogen
Pseudomonas syringae, can be recognized by many plants as a defence elicitor. Responses of
Arabidopsis thaliana suspension ...cells to different HrpZ variants were studied by electrophysiological methods and cell death assay. Purified HrpZ originating from a compatible pathogen
P. syringae pv.
tomato DC3000 (HrpZ
Pto) and incompatible
P. syringae pv.
phaseolicola (HrpZ
Pph) both promoted
Arabidopsis cell death. As an early response, both HrpZ variants induced an increase in time dependent K
+ outward rectifying current. In contrast, the effects of HrpZ proteins on anion currents were different: HrpZ
Pph had no effect, and HrpZ
Pto induced an anion current increase. This suggests that the observed responses of the K
+ channels and anion channels resulted from different and separable interactions and that the interaction implied in anion current modulation is host-specific. HrpZ
Pto and HrpZ
Pph also had a different sequence preference in phage display screen for peptide-binding. These peptides presumably represent a part of a putative target protein in the host, and HrpZ proteins of different
P. syringae pathovars might have different binding specificities to match the allelic variation between plant species. Supporting the idea that the peptide-binding region of HrpZ is important for interactions with host cell components, we found that a mutation in that region changed the anion channel response of
Arabidopsis cells.
► Responses of
Arabidopsis suspension cells to different variants of HrpZ harpin. ► HrpZ from compatible (Pto) and incompatible (Pph) strains of
Pseudomonas syringae. ► HrpZ(Pto) induced an anion current increase while HrpZ(Pph) did not. ► A mutation in HrpZ(Pto) peptide-binding region changed the anion channel response. ► HrpZ(Pto) and HrpZ(Pph) have different sequence preferences for peptide-binding.
This proposal is devoted to a collaborative approach dealing with microwave microscopy experiments. The application is dedicated to an electromagnetic field cartography above circuits and the ...influence of nanometric material layer deposition on the circuits. The first application is associated to a microstrip ring resonator. The results match with the simulated fields. The second application is focused on the effects of a dielectric layer deposited on the circuit and its impact in terms of electromagnetic propagation.
Surgical failure rates after midurethral sling (MUS) procedures are variable and range from approximately 8-57% at five years of followup. The disparity in long-term failure rates is explained by a ...lack of long-term followup and lack of a clear definition of what constitutes failure. A recent Cochrane review illustrates that no high-quality data exists to recommend or refute any of the different management strategies for recurrent or persistent stress urinary incontinence (SUI) after failed MUS surgery. Clinical evaluation requires a complete history, physical examination, and establishment of patient goals. Conservative treatment measures include pelvic floor physiotherapy, incontinence pessary dish, commercially available devices (Uresta
, Impressa
), or medical therapy. Minimally invasive therapies include periurethral bulking agents (bladder neck injections) and sling plication. Surgical options include repeat MUS with or without mesh removal, salvage autologous fascial sling or Burch colposuspension, or salvage artificial urinary sphincter insertion. In this paper, we present the available evidence to support each of these approaches and include the management strategy used by our review panel for patients that present with SUI after failed midurethral sling.
Close shadowing experiments involving natural & synthetic stimuli are described. Preliminary results show that speakers are able to follow natural stimuli with an average delay of 70 ms whereas this ...delay typically exceeds 100 ms for stimuli produced by text-to-speech systems. A complementary experiment shows that this contrast is mainly due to the inappropriate or impoverished prosody generated by actual text-to-speech systems. 1 Table, 4 Figures, 1 Appendix, 29 References. Adapted from the source document
Choosing the right sling for your patient Steele, Stephen S; Bailly, Gregory G
Canadian Urological Association journal,
06/2017, Letnik:
11, Številka:
6Suppl2
Journal Article
Recenzirano
Odprti dostop
Recent data has demonstrated a one in five lifetime risk of a woman requiring stress urinary incontinence (SUI) surgery. Currently, most women opt for a synthetic midurethral sling (MUS), with over ...3.6 million placed worldwide. This article attempts to identify whether a gold standard exists with regards to surgical correction of female SUI. When considering which sling type to use for which incontinent woman, the published data demonstrates excellent results for both synthetic mesh (retropubic or transobturator routes) and fascial pubovaginal slings for most patients. Intrinsic sphincter deficiency does appear to be better treated with the use of a retropubic approach, although still with less than stellar results. With little to differentiate, the treatment of most female SUI may be solely based on which sling the surgeon feels most comfortable performing. Currently, most urologists and gynecologists favour synthetic MUS over fascial slings in surgical-naïve patients; however, recent U.S Food and Drug Administration (FDA) warnings concerning the use of mesh in transvaginal surgery have patients questioning the safety of synthetic MUS for the treatment of SUI.
Audiovisual Speech Synthesis Bailly, G; Berar, M; Elisei, F ...
International journal of speech technology,
10/2003, Letnik:
6, Številka:
4
Journal Article
Recenzirano
This paper presents the main approaches used to synthesize talking faces & provides greater detail on a handful of these approaches. An attempt is made to distinguish between facial synthesis itself ...(ie, the manner in which facial movements are rendered on a computer screen), & the way these movements may be controlled & predicted using phonetic input. The two main synthesis techniques (model-based vs image-based) are contrasted & presented by a brief description of the most illustrative existing systems. The challenging issues - evaluation, data acquisition, & modeling - that may drive future models are also discussed & illustrated by our current work at ICP. 16 Figures, 64 References. Adapted from the source document
Objectives. To assess the association between dietary fat and various urinary risk factors of calcium stone disease in a group of calcium stoneformers attending an outpatient stone clinic.
Methods. ...Mean daily fat intake from self-recorded 4-day dietary food records and mean 24-hour urinary risk factors from two separate collections were evaluated in 476 patients selected randomly from an adult population attending an outpatient stone clinic for the first time.
Results. Mean daily total fat intake for men and women was significantly different at 105.6 and 78.1 g, respectively. Examination of the relationship between total fat intake and 24-hour urinary volume, pH, and excretions of magnesium, citrate, oxalate, calcium, and uric acid revealed no significant regressions in men and only a weak association between fat intake and urinary uric acid in women.
Conclusions. Dietary fat does not have a significant effect on the urinary risk factors of calcium stone disease.
In this contribution to PSAS08 we report on the research activities developed in our Toulouse group in the framework of the BMV project, concerning the search for photon oscillations into massive ...particles, such as axion-like particles, in the presence of a strong transverse magnetic field. We recall our main result obtained in collaboration with LULI at Ecole Polytechnique (Palaiseau, France). We also present the very preliminary results obtained with the BMV experiment which is set up at LNCMI (Toulouse, France). Traduit par la Redaction
Stress incontinence (SUI) and pelvic organ prolapse (POP) are common conditions. There is high-level evidence that midurethral mesh slings for stress incontinence are effective and safe; however, the ...rare but serious potential risks of this surgery must be discussed with the patient. The use of transvaginal mesh for prolapse repair does not appear to be supported by the current evidence, and its use should be restricted to specialized pelvic floor surgeons and specific clinical situations.
PDF
