One advantage that sequencing offers is in the ability to find breakpoints, the sequence boundaries where a structural variant begins and ends. Without knowing the breakpoints, it is hard to track a ...variant across a population and even more difficult to understand the functional impact a variant might have or the mechanisms that produced it.
BLAME IT ON THE ANTIBODIES Baker, Monya
Nature (London),
05/2015, Letnik:
521, Številka:
7552
Journal Article
Recenzirano
Poorly characterized antibodies probably contribute more to the problem than any other laboratory tool, says Glenn Begley, chief scientific officer at TetraLogic Pharmaceuticals in Malvern, ...Pennsylvania, and author of a controversial analysis1 showing that results in 47 of 53 landmark cancer research papers could not be reproduced. Antibodies are produced by the immune systems of most vertebrates to target an invader such as a bacterium. Since the 1970s, scientists have exploited antibodies for research. If a researcher injects a protein of interest into a rabbit, white blood cells known as B cells will start producing antibodies against the protein, which can be collected from the animal's blood.
The strategy for digital PCR (dPCR) has been summarized as divide and conquer: a sample is diluted and partitioned into hundreds or even millions of separate reaction chambers so that each contains ...one or no copies of the sequence of interest. Among other applications, researchers have used digital PCR to distinguish differential expression of alleles, to track which viruses infect individual bacterial cells, to quantify cancer genes in patient specimens and to detect fetal DNA in circulating blood.
Misuse of the P value - a common test for judging the strength of scientific evidence - is contributing to the number of research findings that cannot be reproduced, the American Statistical ...Association (ASA) warned on 8 March.
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