The purpose of the current study was to investigate the effect of dietary dihydroquercetin (DHQ) supplementation on dextran sodium sulfate (DSS)-induced colitis in mice. Mice were given DHQ ...supplementation (3 g kg
) throughout the study, starting 14 days prior to DSS treatment for 1 week followed by 2 days without DSS. The results showed that dietary DHQ supplementation restored DSS-induced disease activity index (DAI), colon length and histopathology scores of the colon tissue. Additionally, supplementation with DHQ reduced the pro-inflammatory cytokine levels, and enhanced the level of IL-10 in the serum. qPCR results indicated that DHQ supplementation significantly downregulated
,
, and
, and upregulated
gene mRNA expression. Western blot results proved that DHQ supplementation upregulated ZO-1 and occludin levels. Using amplicon sequencing technology, 16S rRNA sequencing results showed that DHQ supplementation increased the fecal
/
ratio and the relative abundance of
and
, and decreased the relative abundance of
. Additionally, DHQ supplementation restored the decreased fecal acetic acid and butyric acid concentrations in DSS-induced colitis mice. Besides, Spearman's correlation analysis showed that
was positively correlated with the butyric acid level and
was positively correlated with the mRNA expression of
and
. Both
and
showed a negative correlation with the mRNA expression of
,
, and
, and
was positively correlated with
. In summary, it was found that DHQ supplementation alleviated DSS-induced colitis which may be potentially associated with altered fecal microbiota communities in mice.
Summary of main observation and conclusion
The importance of CF3‐containing molecules in pharmaceuticals, agrochemicals and materials intrigues the intense interest in synthetic methodology of these ...compounds. With a purpose to enrich trifluoromethylation methodology, we carefully examined the substrate scope of hydroxytrifluoromethylation of alkenes using iodotrifluoromethane, and the reaction provided β‐trifluoromethyl alcohols in good yields under extremely mild conditions without catalysts. We found that our reaction can be applied to not only styrenes but also various aliphatic alkenes with excellent selectivity; no ketone was detected in most of our cases. Another feature of our discovery is “simple”. The reaction was carried out in air, irradiated by visible light, at room temperature and most importantly no catalyst was needed. A solution of CF3I in DMSO was used as the facile trifluoromethylating reagent, which simplified the utilization of gaseous CF3I. Based on 19F NMR spectroscopy, we observed a halogen bond between CF3I and tertiary amine in this reaction. The interaction may promote single electron transfer by the visible light irradiation.
The reaction can be applied to not only styrenes but also various aliphatic alkenes with excellent selectivity. We propose a halogen bond interation promotes a single electron transfer under the visble light irradiation. No catalyst was needed. A solution of CF3I in DMSO was used as the facile trifluoromethylating reagent.
Bleached bamboo pulp, as a kind of natural cellulose, has received significant attention in the field of biomass materials due to its advantages of environmental protection and the abundance of raw ...materials. Low-temperature alkali/urea aqueous system is a green dissolution technology for cellulose, which has promising application prospects in the field of regenerated cellulose materials. However, bleached bamboo pulp, with high viscosity average molecular weight (
η) and high crystallinity, is difficult to dissolve in an alkaline urea solvent system, restraining its practical application in the textile field. Herein, based on commercial bleached bamboo pulp with high
η, a series of dissolvable bamboo pulps with suitable
η was prepared using a method of adjusting the ratio of sodium hydroxide and hydrogen peroxide in the pulping process. Due to the hydroxyl radicals being able to react with hydroxyls of cellulose, molecular chains are cut down. Moreover, several regenerated cellulose hydrogels and films were fabricated in an ethanol coagulation bath or a citric acid coagulation bath, and the relationship between the properties of the regenerated materials and the
η of the bamboo cellulose was systematically studied. The results showed that hydrogel/film had good mechanical properties, as the
η is 8.3 × 10
and the tensile strength of a regenerated film and the film have values up to 101 MPa and 3.19 MPa, respectively. In this contribution, a simple method of a one-step oxidation of hydroxyl radicals to prepare bamboo cellulose with diversified
η is presented, providing an avenue for a preparation of dissolving pulp with different
η in an alkali/urea dissolution system and expanding the practical applications of bamboo pulp in biomass-based materials, textiles, and biomedical materials.
Perioperative neurocognitive disorders have been widely recognized as common adverse events after surgical intervention. Aging is one of the most important independent risk factors for worsened ...cognitive outcome, and this deterioration is linked to exacerbated microglia‐mediated neuroinflammation in the aged brain. Under pathological stimulation, microglia are capable of polarizing toward proinflammatory M1 and anti‐inflammatory M2 phenotypes. In the present study, we examined how aging affects microglial responses and neuroinflammation following peripheral surgery. Adult (2‐3 months) and aged (18 months old) male C57/BL6 mice were subjected to tibial fracture or sham surgery. Aged mice exhibited higher level of tumor necrosis factor‐α (TNF‐α) and interleukin‐1β (IL‐1β) in the hippocampus. The expression of synaptic protein synaptophysin (SYP) was also markedly reduced in the aged brain after the surgery. Both adult and aged mice showed significant increases in M1 microglial polarization (CD16/32). In contrast, tibial fracture surgery induced a decreased M2 microglial polarization (CD206, Ym1/2, Arg1) in aged brain but enhanced M2 microglial polarization in adult brain. Aged mice have upregulated voltage‐gated proton channel (Hv1) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunit expression compared with adult mice. The percentage of CD16/32‐positive M1 microglia colabeling with Hv1 was higher in aged mice after tibial fracture surgery. Thus, Hv1/NADPH oxidase upregulation in the aged brain may shift the dynamic equilibrium of microglial activation toward M1 polarization and exaggerate postoperative neuroinflammatory responses after peripheral surgical intervention.
Recent evidences showed that long noncoding RNAs (lncRNAs) are frequently dysregulated and play important roles in various cancers. Clear cell renal cell carcinoma (ccRCC) is one of the leading cause ...of cancer-related death, largely due to the metastasis of ccRCC. However, the clinical significances and roles of lncRNAs in metastatic ccRCC are still unknown.
lncRNA expression microarray analysis was performed to search the dysregulated lncRNA in metastatic ccRCC. quantitative real-time PCR was performed to measure the expression of lncRNAs in human ccRCC samples. Gain-of-function and loss-of-function experiments were performed to investigate the biological roles of lncRNAs on ccRCC cell proliferation, migration, invasion and in vivo metastasis. RNA pull-down, RNA immunoprecipitation, chromatin immunoprecipitation, and western blot were performed to explore the molecular mechanisms underlying the functions of lncRNAs.
The microarray analysis identified a novel lncRNA termed metastatic renal cell carcinoma-associated transcript 1 (MRCCAT1), which is highly expressed in metastatic ccRCC tissues and associated with the metastatic properties of ccRCC. Multivariate Cox regression analysis revealed that MRCCAT1 is an independent prognostic factor for ccRCC patients. Overexpression of MRCCAT1 promotes ccRCC cells proliferation, migration, and invasion. Depletion of MRCCAT1 inhibites ccRCC cells proliferation, migration, and invasion in vitro, and ccRCC metastasis in vivo. Mechanistically, MRCCAT1 represses NPR3 transcription by recruiting PRC2 to NPR3 promoter, and subsequently activates p38-MAPK signaling pathway.
MRCCAT1 is a critical lncRNA that promotes ccRCC metastasis via inhibiting NPR3 and activating p38-MAPK signaling. Our results imply that MRCCAT1 could serve as a prognostic biomarker and therapeutic target for ccRCC.
In this paper, a liquid crystal (LC) tunable origami metastructure (OMS) designed for curvature sensing on cylindrical surfaces to measure their curvature is introduced. The LC employed is K15 (5CB) ...and the applicable band is 0.36∼23 GHz. When excited by electromagnetic waves (EMWs) within the 4∼16 GHz, the resonance frequency of the OMS shifts from 10.24 GHz to 10.144 GHz, corresponding to a change in absorption amplitude ranging from 0.773 to 0.920. In terms of curvature sensing, the detectable range of curvature spans from 0 to 0.327 mm
. The maximum sensitivity (S) achieved for curvature measurement reaches 0.918/mm
, accompanied by a quality factor (Q-factor) of 25.88. The proposed OMS embodies numerous excellent traits, including wide-range sensing capabilities and heightened S, promising for applications in bionic skin, smart robotics, and related fields.
This study investigates the feasibility of distributed fiber optic sensor for corrosion monitoring of steel bars embedded in concrete. Two sensor installation methods are compared: (1) attaching the ...sensor along the bar and (2) winding the sensor on the bar. For the second method, optical fibers were winded spirally on steel bars with different spacings: 0 mm, 2 mm, 5 mm, and 10 mm. Steel bar pull-out testing was conducted to evaluate the effect of presence of distributed sensor on the bond strength of steel⁻concrete interface. Electrochemical testing was carried out to assess the influence of the installation methods on the corrosion resistance of the reinforced concrete. Winding the optical fiber on steel bars with a 10-mm spacing does not affect the bond strength and corrosion resistance and allows real-time corrosion monitoring. The distributed sensor data can be used to estimate the corrosion induced steel loss and predict concrete cracking.
This study aimed to examine the reporting quality of existing economic evaluations for negotiated glucose-lowering drugs (GLDs) included in China National Reimbursement Drug List (NRDL) using the ...Consolidated Health Economic Evaluation Reporting Standards 2013 (CHEERS 2013).
We performed a systematic literature research through 7 databases to identify published economic evaluations for GLDs included in the China NRDL up to March 2021. Reporting quality of identified studies was assessed by two independent reviewers based on the CHEERS checklist. The Kruskal-Wallis test and Mann-Whitney U test were performed to examine the association between reporting quality and characteristics of the identified studies.
We have identified 24 studies, which evaluated six GLDs types. The average score rate of the included studies was 77.41% (SD:13.23%, Range 47.62%-91.67%). Among all the required reporting items, characterizing heterogeneity (score rate = 4.17%) was the least satisfied item. Among six parts of CHEERS, results part scored least at 0.55 (score rate = 54.79%) because of the incompleteness of characterizing uncertainty. Results from the Kruskal-Wallis test and Mann-Whitney U test showed that model choice, journal type, type of economic evaluations, and study perspective were associated with the reporting quality of the studies.
There remains room to improve the reporting quality of economic evaluations for GLDs in NRDL. Checklists such as CHEERS should be widely used to improve the reporting quality of economic researches in China.
•Thermal properties of HPFRCCs after exposure to high temperatures.•Mechanical properties of HPFRCCs after exposure to high temperatures.•Effects of mix proportions on thermal properties of ...HPFRCCs.•Effects of mix proportions on mechanical properties of HPFRCCs.
This paper investigates residual thermal and mechanical properties of high-performance fiber-reinforced cementitious composites (HPFRCCs) proportioned with high-volume fly ash after exposure to 200, 400, 600, and 800°C. The investigated thermal and mechanical properties include the thermal conductivity, specific heat, compressive strength, tensile strength, tensile ductility, and density. The effects of five mix proportioning variables on the material properties are evaluated, which include the water-to-binder ratio (0.24–0.36 by mass), sand-to-binder ratio (0.36–0.66 by mass), fly ash content (60%–75% by mass), polyvinyl alcohol fiber content (1.5%–2.2% by volume), and superplasticizer content (0.10%–0.15% by mass). Experimental results reveal significant dependence of the residual thermal and mechanical properties on temperature and the mix proportion, which can be attributed to a series of chemical and physical reactions that occur at elevated temperatures. This study can facilitate optimization of mix proportions of HPFRCCs for enhanced thermal and mechanical performance in fire or high temperature applications.
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