Currently, one of the topical areas of research is the development of new antigen preparations for the specific diagnosis and prevention of brucellosis, since indication of the pathogen and ...prevention of the disease is complicated by the ability of brucella to dissociate, and live vaccines used for specific prevention of brucellosis have residual virulence. Thermal extracts (TE) obtained from Brucella abortus I-206 in the L- and S-form can be used as such promising antigens. It is known that TE in the L- and S-forms have immunogenic properties, as well as a modulating effect on the proliferation of immunocompetent cells, morphological changes in the immunocompetent organs of experimental animals.
The aimof
the work is to study the effect of Brucella abortus thermal extracts in L- and S-forms on the functional state of the cells of experimental animals.
Materials and methods
. The study was performed on 100 certified white mice. As objects of study, we used the B. abortus I-206 TE in L- and S-forms. Evaluation of the effect of antigenic drugs on the functional state of phagocytes of laboratory animals in vitro was performed on peritoneal macrophages. The total activity of the respiratory chain enzymes in the NBT-test and superoxide dismutase was determined. Cells of intact animals served as controls. As a positive control, a commercial antigenic LPS preparation Escherichia coli was used. The content of cyclic nucleotides in homogenates of immunocompetent organs was determined using ELISA.
Results.
This study presents materials on the study of the effect of TE on the bactericidal activity of phagocytes and the level of cyclic nucleotides in immunocompetent organs. It has been established that TEs activate oxygen-dependent bactericidal systems of phagocytes. When studying the effect of TE on the content of cyclic nucleotides in immunocompetent organs of white mice, an increase in their concentration was revealed, indicating an increase in the functional activity of the cells.Conclusion.The obtained data make it possible to substantiate the need for a further detailed study of the immunogenic properties of B. abortus TE in the L- or S-form on the organism of experimental animals.
We discuss theoretical description of pressure-induced phase transitions by means of first-principles calculations in the framework of density functional theory. We illustrate applications of ...theoretical tools that allow one to take into account configurational and vibrational disorders, considering Ti-V alloys as a model system. The universality of the first-principles theory allows us to apply it in studies of different phenomena that occur in the Ti-V system upon compression. Besides the transitions between different crystal structures, we discuss isostructural transitions in bcc Ti-V alloys. Moreover, we present arguments for possible electronic transitions in this system, which may explain peculiar behaviour of elastic properties of V upon compression.
The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon,
Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography ...(HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH
2.
Blood serum cortisol (F), testosterone (T), 11-ketotestosterone (11KT), oestradiol-17 (E2), progesterone (P4), 17,20,21-trihydroxy-4-pregnen-3-one (20'S) and 17,20'-dihydroxy-4-pregnen-3-one ...(17,20'P) concentrations were measured by enzyme linked immuno-sorbent assay (ELISA) and radio-immunoassay (RIA) in stellate sturgeon (Acipenser stellatus, Pallas) during final maturation (FM) induced by luteinizing hormone-releasing hormone analogue (LH-RH-A). After the LH-RH-A treatment there was a significant elevation of sex steroids (T, E2, P4, 20'S) and F levels before ovulation and decrease of T and E2 levels after ovulation. In males at the beginning of spermiation LH-RH-A induced a rise of sex steroids and F levels. There was a decrease of steroids levels at the end of spermiation. The data indicates a possible role of 20'S takes part in FM in stellate sturgeon male as well as in female. The rise in F levels may indicate an interaction between corticosteroids and gamete maturation in stellate sturgeon.
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