•A new Schiff base ligand N1, N5-bis2-hydroxyphenyl-2-methyl-1,5-diaminopentane is synthesized and characterized.•In-Silico studies of compounds to support the in-vitro results•The increase in the ...spacer group in Schiff base compounds increases the biological activities
A novel ONNO donor tetradentate Schiff base ligand N,N'-bis2-hydroxyphenyl-2-methyl-1,5-diaminopentane (1) is synthesized and characterized by using 1H, 13C NMR spectroscopy and mass spectrometry. Biological studies on newly synthesized ligand are carried out and results are compared with the biological activities of known Schiff base ligands: N,N'-bis{2-hydroxyphenyl}-1,4-diaminobutane (2) and N,N'-bis{2-hydroxyphenyl}-1,3-diaminopropane (3). This comparative study highlights the effect of increasing methylene linkage on the biological activity of these ligands. The anti-microbial activities of all three ligands have been screened against two distinct gram-positive and gram-negative bacteria. In-vitro results of this comparative analysis are also supported by comprehensive molecular docking studies with DNA gyrase subunit B protein of gram-positive bacterium S. aureus and FabH-CoA complex of gram-negative bacterium E. coli. Experimental studies reveal that the spacer group plays a vital role in controlling the biological activities of the ligand. These findings are in good agreement with the theoretical findings. The study demonstrates that the biological activity of ligands is enhanced with increments in the number of spacers. Thus, the newly synthesized ligand (1) with the most extended spacer among the three showed the highest biological activity compared to other two ligands, similar to the popular drug Ciprofloxacin.
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•Biocompatible fluorescent N,K,Ca-doped CQDs derived from Chenopodium album.•Fluorescence quantum yield up to 48.12 % was achieved.•Bioimaging of SH-SY5Y human neuroblastoma cells, ...bacteria, fungal, and plant cells.•The cytotoxicity and cell viability have also been examined.
This study demonstrated an economical, ecologically sustainable, and simple method for producing biocompatible fluorescent carbon quantum dots (CQDs) co-doped with N, K, and Ca from bathua (Chenopodium album), a popular medicinal herb leaf extract and nutritious vegetable. The nearly spherical shaped CQDs were discovered to be 3–5 nm in size in nature, with a quantum yield of around 48.12%, robust photo-stability, and excellent water solubility. Various morphological, compositional, physicochemical and optical characteristics were investigated. Additional factors affecting the photoluminescence of CQDs, such as hydrothermal process parameters, pH, and solvent sensitivity, were investigated further. Moreover, the newly synthesized N, K, Ca-doped CQDs were demonstrated to be a useful fluorescent nanoprobe for in vitro cellular imaging of SH-SY5Y human neuroblastoma cells, as well as other living organisms such as bacteria, fungal, and plant cells, as a cost-effective alternative to organic or synthetic fluorescent probes. Cell survival and cytotoxicity were found to be acceptable, with 95% of SH-SY5Y and HCT-116 cells surviving and other organisms able to withstand higher doses (10.9 mg/mL) for up to 4 days.
This study demonstrated an environmentally responsible, inexpensive and simple one-step hydrothermal method to synthesize biocompatible fluorescent carbon quantum dots (CQDs) from Aegle marmelos ...fruit extract. The near-spherical CQDs were discovered to have a naturally distributed size of 3–6 nm, were unintentionally doped with heteroatoms (Ca, K, and N) without the need for doping element precursors due to mineral contents in extract, had a quantum yield of about 4.06%, strong photostability, and excellent water solubility. Several morphological, compositional, physicochemical, and optical properties were studied. Other variables influencing CQD photoluminescence, such as hydrothermal process parameters and pH sensitivity, were also examined. Zeta potential analysis showed a negative surface charge with a zeta potential value of −5.29 mV and good dispersibility in aqueous solution. Furthermore, as an alternative to organic or synthetic probes, there is a huge need for the development of simple, affordable, and non-destructive fluorescent biomarkers. As-synthesized Ca, K, N-doped CQDs have demonstrated good fluorescent staining abilities and may be employed as a helpful probe for bio-imaging of cancer cells and other organisms. Cell survival and cytotoxicity were found to be satisfactory, with 90% of SH-SY5Y and HCT-116 cells surviving, compared to 63–77% for the other organisms, even at higher dosages (200 µg/mL) for up to 4 days.
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•A facile heteroatom doped CQDs have been synthesized from Aegle marmelos.•Hydrothermal process dependent and pH responsive PL were examined.•Bio-imaging of cancer cells and other living organisms are demonstrated.•Cytotoxicity were found over 90% of SH-SY5Y and HCT-116 cancer cells.
Stroke is a complex multifactorial disease influenced by several genetic and environmental factors. Vitamin B12 deficiency is a major public health problem in Indian population due to adherence to ...strict vegetarian diet. Transcobalamin II is a key protein involved in intracellular transport of vitamin B12. So, genetic variation in transcobalamin II gene may lower concentration of intracellular vitamin B12 and may increase the susceptibility of stroke. The objective of the present study was to explore the effect of transcobalamin II gene polymorphism upon susceptibility to stroke in Indian population. A case-control study was conducted in Department of Biochemistry and Medicine, VMMC and SJH, New Delhi, which included 32diagnosed cases of stroke and 32 age and sex matched healthy control subjects. Genomic DNA isolation was done by DNA extraction Kit. The genotyping for transcobalamin II SNP (rs4820889 (A right arrow G)) was done by Allele Specific Polymerase chain reaction. Among cases, genotype frequency of AA is 75%, AG is 18.8%, GG is 6.3% and allelic frequency of A is 84.4% and G is 15.6%. Among controls, genotype frequency of AA is 93.8%, AG and GG is 3.1% and allelic frequency of A is 95.3% and G is 4.7%. Pearson Chi-Square p-value = 0.0389, OR = 5.0;95% = 0.96-25.78. In our study we have found that transcobalamin II gene SNP rs4820889 is significantly (p-value <0.05) associated with the risk of stroke.
INTRODUCTION: Breast cancer is the most common cancer in women worldwide. Risk of cancer increases with respect to age and menopausal status. Serum tumor markers are important tools in management of ...patients with breast cancer. Likewise, Lerritin belongs to a group of other molecules of potential interest whose concentration is also altered in sera of breast cancer patients. In the present study, the serum ferritin concentration was estimated in the sera of breast cancer patients before any surgical or any other treatment and compared to healthy women as controls. AIM AND OBJECTIVES: The aim of this study was to assess serum ferritin concentration in breast cancer patients and to correlate it with menopausal status of the patient. MATERIALS & METHODS: Histopathologically confirmed, 50 breast cancer female cases and age & sex matched healthy female controls were taken from surgery OPD, over a period of one year. Level of ferritin was estimated by Sandwich Elisa using Ferritin SA Elisa kit. RESULT: Serum ferritin level in breast cancer cases (281.83 + or - 39.037 ng/ml) is statistically higher than in controls (87.19 + or - 43.69ng/ml) p<0.001. In breast cancer, ferritin level was higher in postmenopausal (300.73 + or - 25.33ng/ml) compared to premenopausal (228.059 + or - 11.24ng/ml) patients (p<0.001). In healthy women, statistically significant difference (p<0.001) in ferritin concentration was observed between premenopausal (51.72 5 + or - 18.5 8ng/ml) and postmenopausal women (125.79 + or - 25ng/ml). CONCLUSION: Serum Ferritin Level can be used as a prognostic marker for breast cancer and its Severity and its level is elevated in Postmenopausal Group in both breast cancer patients and healthy cases and controls.
Insects can address sustainability issues associated with current food systems by providing an alternative protein source to address hunger and disease. Only the production systems that rely on ...side-stream heat and alternate energy sources may benefit from replacing compound feed production with insect value chains. Seventy-five percent to 93% of the effects of compound feed production on global warming potential, land use, and fossil resource shortages can be avoided. To fully assess the potential of insect production, it is critical to consider a wide range of sustainability indicators, including social, economic, and environmental aspects.
Thermally activated perlite‐supported cobalt oxide (Co‐TAP) catalyst has been synthesized via the deposition‐precipitation method. FTIR peaks at around 663 and 565 cm–1 and XRD peaks arise from ...diffraction of FCC planes of CoO crystals confirm the presence of Co3O4 crystallites on Co‐TAP surface. UV–Vis DRS studies reveal the presence of octahedrally coordinated Co3+ and tetrahedrally coordinated Co2+ ions. EDX analysis of Co‐TAP clearly shows the enhanced percentage of silica and cobalt oxide. All characterization studies suggest the presence of Lewis acidic sites on Co‐TAP which have been utilized in a series of liquid‐phase esterification reactions of benzoic acid with various alcohols over three different reaction media (conventional, microwave, and sonication). The reactions are carried out in one‐pot, single‐step, solvent‐free reaction conditions, and the catalyst is filtered easily, regenerated, and reused up to five reaction cycles with analogous efficiency suggesting that acidic sites of the catalyst remain stable in all reaction mediums. The stability of regenerated Co‐TAP catalyst after the fifth reaction cycle is confirmed by FTIR spectrum which resembles the FTIR of fresh catalyst.
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•We report a simple, cost-effective, eco-sustainable synthesis of fluorescent carbon quantum dots derived from precursor Cissus quadrangularis.•The as-synthesized nitrogen doped-CQDs ...demonstrated a homogeneous size distribution (4–9 nm), green fluorescent nature, great photo-stability, excellent water solubility, and a quantum yield of around 5 %.•A biodegradable wheat-starch-based bioplastic was employed to fabricate an N-doped CQDs@bioplastic composite to demonstrate its potential applications in quantum dot-based optical displays and down-conversion light emitting diodes.•The influence of different CQD concentrations and pH sensitivity were investigated further utilizing fabricated optical displays and LEDs.
Carbon quantum dots (CQDs)-based composites as luminous down-conversion materials are becoming more popular due to several advantages such as steady fluorescence, ease of functionalization, tailoring of emission in the visible range, and so on. We report an inexpensive and environmentally sustainable synthesis of fluorescent nitrogen doped-CQDs produced from Cissus quadrangularis, a low-cost plant precursor with therapeutic value. The morphological, structural, and physicochemical features of the material were carefully investigated. Under UV stimulation (365 nm), almost spherical shaped N-CQDs with an average diameter of 5.1 nm were discovered to generate yellow-green fluorescence, have excellent photostability, and strong water solubility, with a quantum yield of up to 5 %. Furthermore, as a solid-phase dispersion matrix for CQDs, ecologically friendly and biodegradable bioplastic is appealing. The down-conversion of solid-state fluorescence of LEDs and UVC tubes was demonstrated by creating a nanocomposite by inserting N-CQDs into the solid matrix of a wheat starch-based bioplastic. Furthermore, employing constructed quantum dot-based optical displays, down-converted LEDs, and UVC tubes, the impacts of varied CQD concentrations and pH sensitivity were examined.
In the present study, perlite is converted into a potentially active heterogeneous base catalyst, by thermal and chemical activation using NaOH. The physio-chemical properties of as synthesized ...catalyst were monitored by XRD, FT-IR, SEM, SEM-EDAX. The efficiency of the prepared catalyst was evaluated by condensation reactions. The catalyst could be easily recovered and re-utilized up to 4 reaction cycles giving almost analogous yield %, indicating its sustainability under reaction conditions. The main purpose was to study conversion of silica–rich perlite into potential heterogeneous base catalyst for base catalyzed organic reactions, which could be a promising route to utilize this abundant waste material.
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