Background: Platelet concentrated is used in regenerative medicine for its high content in growth factors and proteins. However, the need of autologous blood and the lack ofstandard protocols limits ...its clinical use. Using platelet derived-extracellularvesicles (EVs), such as exosomes (30-100 nm) or microvesicles (100-1000 nm), are an alternative to platelet concentrated due to their advantages since no autologous blood is needed and can be sterilized by filtration and stored until use. Our aim was to test if platelet lysate and platelet-derived EVs extracted by different methods exerted the same effect on the differentiation of the pre-osteoblastic cell line MC3T3-E1. Methods: Platelet-derived EVs were isolated by different methodologies: polyethylene glycol (PEG) precipitation, ultracentrifugation or the commercial kit Exo-Spin™. The obtained EVs were characterized in terms of size by TEM (transmission electron microscopy), DLS (dynamic light scattering), AFM (atomic force microscopy) and for the presence of EVs markers by Western blot. Five micrograms of isolated EVs or platelet lysate were used to treat MC3T3-E1 cells for 48 h and the effect in metabolic activity was studied by resazurin reduction. Results: Exosomes isolation by PEG precipitation allows the obtaining of smaller size particles with a higher protein concentration compared to the other evaluated methods. In addition, platelet lysate and exosomes obtained by PEG precipitation lead to a similar metabolic activity on mouse pre-osteoblasts. Summary/Conclusion: Thus, the platelet lysate effect on the cells could be due to the EVs present, suggesting that platelet-derived EVs could be used as alternative to platelet concentrates.
Hemorragia intraventricular após embolização de fístula dural de Carvalho, Joana Chaves Gonçalves Rodrigues; Machin, Francisco Javier Tercero; Manzanera, Luis San Roman ...
Revista brasileira de anestesiologia,
March-April 2017, 2017-03-00, Letnik:
67, Številka:
2
Journal Article
Recenzirano
Odprti dostop
Fístulas arteriovenosas durais (FAVD) são comunicações anômalas entre os canais venosos e arteriais da dura‐máter cujo centro está localizado entre os folhetos da dura‐máter. Para as circunstâncias ...nas quais o tratamento invasivo é obrigatório, as técnicas endovasculares se tornaram os pilares da prática, escolha atribuível a relatos de sua segurança e eficácia. Descrevemos o caso único e raro de uma FAVD tratada por embolização transarterial (ETA) e complicada por uma hemorragia intraventricular (HIV). Nosso objetivo foi destacar alguns aspectos centrais do manejo perioperatório desses pacientes para ajudar a melhorar uma futura abordagem de casos semelhantes.
Paciente do sexo feminino, 59 anos, com diagnóstico prévio de FAVD tipo IV (Cognard), apresentou‐se para ETA, realizada fora da sala de cirurgia sob anestesia venosa total. O procedimento transcorreu sem complicações, e a angiografia intraoperatória revelou obliteração completa da fístula. No período pós‐operatório imediato, a paciente apresentou sinais clínicos de aumento da pressão intracraniana (PIC) atribuíveis a uma HIV posteriormente diagnosticada, o que condicionou a colocação de um dreno ventricular, internação em Unidade de Terapia Intensiva (UTI), vasoespasmo cerebral e internação hospitalar prolongada. Durante todo o período perioperatório, não houve alterações na oximetria cerebral. A paciente recebeu alta sem sequelas neurológicas.
HIV pode ser uma complicação grave após o tratamento endovascular de FAVD. A observação e o monitoramento cuidadosos no pós‐operatório permitem o diagnóstico precoce e o tratamento que aumenta as chances de um resultado melhor.
Dural arteriovenous fistulas are anomalous shunts between dural arterial and venous channels whose nidus is located between the dural leaflets. For those circumstances when invasive treatment is mandatory, endovascular techniques have grown to become the mainstay of practice, choice attributable to their reported safety and effectiveness. We describe the unique and rare case of a dural arteriovenous fistula treated by transarterial embolization and complicated by an intraventricular hemorrhage. We aim to emphasize some central aspects of the perioperative management of these patients in order to help improving the future approach of similar cases.
A 59‐year‐old woman with a previously diagnosed Cognard Type IV dural arteriovenous fistula presented for transarterial embolization, performed outside the operating room, under total intravenous anesthesia. The procedure underwent without complications and the intraoperative angiography revealed complete obliteration of the fistula. In the early postoperative period, the patient presented with clinical signs of raised intracranial pressure attributable to a later diagnosed intraventricular hemorrhage, which conditioned placement of a ventricular drain, admission to an intensive care unit, cerebral vasospasm and a prolonged hospital stay. Throughout the perioperative period, there were no changes in the cerebral brain oximetry. The patient was discharged without neurological sequelae.
Intraventricular hemorrhage may be a serious complication after the endovascular treatment of dural arteriovenous fistula. A close postoperative surveillance and monitoring allow an early diagnosis and treatment which increases the odds for an improved outcome.
Intraventricular hemorrhage after dural fistula embolization Joana Chaves Gonçalves Rodrigues de Carvalho; Francisco Javier Tercero Machin; Luis San Roman Manzanera ...
Brazilian journal of anesthesiology (Elsevier),
03/2017, Letnik:
67, Številka:
2
Journal Article
Odprti dostop
Background and objectives: Dural arteriovenous fistulas are anomalous shunts between dural arterial and venous channels whose nidus is located between the dural leaflets. For those circumstances when ...invasive treatment is mandatory, endovascular techniques have grown to become the mainstay of practice, choice attributable to their reported safety and effectiveness. We describe the unique and rare case of a dural arteriovenous fistula treated by transarterial embolization and complicated by an intraventricular hemorrhage. We aim to emphasize some central aspects of the perioperative management of these patients in order to help improving the future approach of similar cases. Case report: A 59-year-old woman with a previously diagnosed Cognard Type IV dural arteriovenous fistula presented for transarterial embolization, performed outside the operating room, under total intravenous anesthesia. The procedure underwent without complications and the intraoperative angiography revealed complete obliteration of the fistula. In the early postoperative period, the patient presented with clinical signs of raised intracranial pressure attributable to a later diagnosed intraventricular hemorrhage, which conditioned placement of a ventricular drain, admission to an intensive care unit, cerebral vasospasm and a prolonged hospital stay. Throughout the perioperative period, there were no changes in the cerebral brain oximetry. The patient was discharged without neurological sequelae. Conclusion: Intraventricular hemorrhage may be a serious complication after the endovascular treatment of dural arteriovenous fistula. A close postoperative surveillance and monitoring allow an early diagnosis and treatment which increases the odds for an improved outcome. Resumo: Justificativa e objetivos: Fístulas arteriovenosas durais (FAVD) são comunicações anômalas entre os canais venosos e arteriais da dura-máter cujo centro está localizado entre os folhetos da dura-máter. Para as circunstâncias nas quais o tratamento invasivo é obrigatório, as técnicas endovasculares se tornaram os pilares da prática, escolha atribuível a relatos de sua segurança e eficácia. Descrevemos o caso único e raro de uma FAVD tratada por embolização transarterial (ETA) e complicada por uma hemorragia intraventricular (HIV). Nosso objetivo foi destacar alguns aspectos centrais do manejo perioperatório desses pacientes para ajudar a melhorar uma futura abordagem de casos semelhantes. Relato de caso: Paciente do sexo feminino, 59 anos de idade, com diagnóstico prévio de FAVD tipo IV (Cognard), apresentou-se para ETA, realizada fora da sala de cirurgia sob anestesia venosa total. O procedimento transcorreu sem complicações, e a angiografia intraoperatória revelou obliteração completa da fístula. No período pós-operatório imediato, a paciente apresentou sinais clínicos de aumento da pressão intracraniana (PIC) atribuíveis a uma HIV posteriormente diagnosticada, o que condicionou a colocação de um dreno ventricular, internação em Unidade de Terapia Intensiva (UTI), vasoespasmo cerebral e internação hospitalar prolongada. Durante todo o período perioperatório, não houve alterações na oximetria cerebral. A paciente recebeu alta sem sequelas neurológicas. Conclusão: HIV pode ser uma complicação grave após o tratamento endovascular de FAVD. A observação e monitoramento cuidadosos no pós-operatório permitem o diagnóstico precoce e o tratamento que aumenta as chances de um resultado melhor. Keywords: Intraventricular hemorrhage, Arteriovenous malformation, Dural fistula, Cerebral perfusion, Cerebral oximetry, Palavras-chave: Hemorragia intraventricular, Malformação arteriovenosa, Fístula dural, Perfusão cerebral, Oximetria cerebral
The faunal analysis of level J has been carried out using a zooarcheological method with some contributions from the field of Taphonomy. The data were obtained from the anatomical and taxonomical ...analysis and from the structural modifications of the bones. Red deer (Cervus elaphus) and horses (Equus ferus) are the most abundant animals, although they are accompanied by other herbivores like Bos primigenius, Stephanorhinus hemitoechus and Rupicapra pyrenaica. The data indicate that level J is an anthropic accumulation with a minimal incidence of carnivores. Carcasses were selectively transported into the site, where they were processed and consumed. The temporal dimension and the possibility that the faunal assemblage may be the result of different occupational events have been also considered.
Taphonomy of Level J of Abric Romaní Cáceres, Isabel; Bennàsar, Maria; Huguet, Rosa ...
High Resolution Archaeology and Neanderthal Behavior
Book Chapter
The taphonomic study of level J (sublevels Ja and Jb) includes macrovertebrates, microvertebrates, wood and charcoal. The association of level J has, in general, a good state of conservation, ...although there are several taphonomic modifications. No mechanisms or taphonomic processes which may have distorted any of these associations have been detected. This study shows how Neanderthals were the main collecting agent of animals and wood and the main agent of modification. Moreover, the water activity (water flow, dry periods and reactivitation) were the main postdepositional agent. The burial of the remains was probably relatively fast in both sublevels. There are abundant similarities in the temporary sequences of formation in both sublevels. The fossilization process of level J is consistent with a karst taphosystem in a travertine deposit. The main feature of this taphosystem is a microenvironment characterized by a relative humid environment, marked by wet conditions and reactivations of water flows. Once the remains were buried, the fossildiagenetic phase was tremendously preservative and allowed the exceptional conservation of the remains found at the site.
PDF
