A comparative assessment of radioprotective properties of inosine nucleoside (riboxin) and recognized radioprotector indralin was carried out. We analyzed survival of male ICR CD-1 mice weighting ...32.2±0.2 g exposed to external X-ray radiation at doses 6.5 and 6.75 Gy and receiving indralin at a dose of 100 or 150 μg/g body weight or riboxin (inosine) at a dose of 100 or 200 μg/g body weight before irradiation. The survival analysis was carried out by the Kaplan—Meier method. The significance was assessed by using the log-rank-test. Inosine showed a significant difference from the irradiated control only at a dose of 100 μg/g body weight at a radiation dose of 6.75 Gy. The survival of animals treated with indralin was significantly higher in comparison with not only the irradiated control group, but also with the groups receiving inosine.
The aim of this study was to develop and validate a patient-rated questionnaire for the complex express-assessment of alcohol anosognosia. The questionnaire was tested in 106 inpatients diagnosed ...with alcohol dependence (ICD-10, item F10.2). The age of the study participants (M+/-SD) was 42.1+/-8.8 years, duration of alcohol dependence 11.3+/-7.0 years. The questionnaire consisted of 7 subscales: Not being in the know, Non-recognition of disease symptoms, Non-recognition of disease in whole, Non-recognition of disease consequences, Emotional rejection of disease, Disagreement with treatment, Non-acceptance of temperance. Psychometric characteristics (external-criterion validity, test-retest validity, internal consistency) were studied and the standardization of the questionnaire was performed. Mean empiric values of subscales were determined. The questionnaire allows to get the complex assessment of anosognosia level by separate components and to present the profile of alcohol anosognosia as well.
Intraindividual response time variability (RTV) is considered as a general marker of neurological health. In adults, the central executive and salience networks (task-positive networks, TPN) and the ...default mode network (DMN) are critical for RTV. Given that RTV decreases with growing up, and that boys are likely somewhat behind girls with respect to the network development, we aimed to clarify age and sex effects. Electroencephalogram was recorded during Stroop-like test performance in 124 typically developing children aged 5-12 years. Network fluctuations were calculated as changes of current source density (CSD) in regions of interest (ROIs) from pretest to 1-s test interval. In boys, TPN activation (CSD increase in ROIs included in the TPN) was associated with lower RTV, suggesting a greater engagement of attentional control. In children younger than 9.5 years, higher response stability was associated with the predominance of TPN activation over DMN activation (CSD increase in ROIs included in the TPN > that in the DMN); this predominance increased with age, suggesting that variability among younger children may be due to network immaturity. These findings suggest that the TPN and DMN may play different roles within the network mechanisms of RTV in boys and girls and at different developmental stages.
Tests of a new filler of the Magram P88 brand produced by the Magnezit Group are conducted. Hot lining repair is conducted on arc furnace No. 2 of BMZ — Management Company BMK Holding of the filler ...of the brand Magram P88 with 2.5 wt.% temporary organic binder amounted to 18 melts, and with 1.5 wt.% to13 melts. The Magram P88 brand filler, compared with that of the Martenit-Om brand, is laid more evenly, having greater adhesion to the working lining of banks, and also its cost is 30% lower.
Objective: Intraindividual variability in response time (RT) provides information about attention abilities beyond accuracy and mean RT. It could be an endophenotype for a wide range of clinical ...disorders and a general marker of neurological health or maladaptation. The default mode network (DMN) and the central executive and the salience networks (CEN + SN) support response stability in adults. The aim of this study was to explore the associations of resting-state connectivity of the DMN and the CEN + SN with lapses of attention in typically developing primary schoolchildren. Method: RT variability was measured by RT standard deviation in the self-paced Stroop-like Animal Size Test in 137 children aged 6-11 years. Electrophysiological signatures of the DMN and the CEN + SN were obtained using a seed-based oscillatory power envelope correlation in conjunction with beamformer spatial filtering. Results: Statistical nonparametric mapping showed that RT variability was positively associated with connectivity between the DMN and the posterior insula and the right temporal cortex (PFWE-corr < .006), suggesting that a greater focus of self-reflective internal attention may be associated with obstructed transition to an active processing mode. RT variability was negatively associated with connectivity between the DMN and the anterior cingulate and the anterior insula (PFWE-corr = .001) overlapping with the SN, which handles top-down cognitive regulation. These associations were specifically found for the anterior DMN seed in the medial prefrontal cortex, which is involved in emotion and motivational circuits. Conclusions: Default network connectivity supporting RT variability may be involved in the development of internalizing and externalizing problems in childhood.
Key Points
Question: Does the organization of brain networks at rest support behavioral stability in typically developing primary schoolchildren? Testing via electrophysiological signatures of networks. Findings: Greater response time variability was associated with stronger default mode network connectivity with surrounding structures and weakening of cooperation between the default mode network and control networks; these associations were specific to the anterior default mode network seed in the medial prefrontal cortex, involved in emotion and motivational circuits. Importance: Default mode network connectivity, specifically involving medial prefrontal cortex, supports response time variability and may be involved in the development of internalizing and externalizing problems in childhood. Next Steps: Investigation of changes in connectivity and balance between default and control networks induced by attention task performance will extend our understanding of the neural mechanisms of behavioral stability.
Nucleoid-associated proteins (NAPs) control the structure and functions of bacterial nucleoid. Histone-like HU proteins are most abundant NAPs in dividing bacterial cells. Previously, structural ...ensembles of conformations of HU proteins from pathogenic mycoplasmas
Spiroplasma melliferum
and
Mycoplasma gallisepticum
were obtained using NMR spectroscopy. A structural study of these mycoplasma proteins is performed by small-angle X-ray scattering (SAXS). The occurrence of individual conformations from the ensemble, obtained by NMR, is estimated from the scattering data on HU protein solutions. In particular, an approach based on characterization of equilibrium mixtures in terms of volume fractions of their components was applied. The general shape of the proteins and their oligomeric state are independently confirmed using ab initio bead modelling. The flexibility of DNA-binding protein domains is analyzed by the ensemble optimization method, which is based on comparison of the structural characteristics of conformations fitting the SAXS data to the distribution of these characteristics in a randomly generated set. The results obtained give a new insight on the variability of the structure of HU proteins, which is necessary for their functioning.
The paper presents an analytical method of modular buildings calculation. It focuses upon basic design assumptions that are accepted for modular buildings calculation. The authors introduce their ...classification of loads and impacts, which are basic for modular buildings. They also describe a method of determining inertial forces from seismic action designed on the basis of a cantilever analytical model. Analytical and numerical methods for determining the forces in the elements of modular units are further compared. It is revealed that the analytical method strongly agrees with FEM (that is the finite elements method) with the error being 2-6%. The analytical method is sufficiently accurate for engineering calculations.
The reaction between spillover hydrogen (SH) and mexidol upon high-temperature solid state catalytic isotope exchange (HSCIE) is studied experimentally and via DFT B3LYP and MP2 calculations. ...The distribution of isotopes and the activation energy of the reaction are determined via NMR spectro-scopy and mass spectrometry for samples of the deuterium-labeled mexidol obtained via HSCIE at 50–120°C. The DFT calculated activation energy of hydrogen transfer on the charged alumina cluster HH
+
(‒Al–O–)
3
(OH)
6
is found to agree with the experimental data. A mechanism is proposed for HSCIE between the adsorbed compound and charged Brønsted acid sites formed on the surface of the support upon exposure to hydrogen spillover.
Study of circulating 02_AG recombinant form HIV-1 isolates that have been rapidly spreading in Novosibirsk region during 3 recent years.
WHO protocol for primary HIV isolation was used, automatic ...sequencer was used for genetic characterization of isolates. Virus specific RNA were isolated and env HIV-1 region DNA fragments were processed. Phylogenetic analysis was also performed.
CRF_02AG HIV-1 isolated from peripheral blood of HIV-1 positive patients belonged to CCR5 tropic viruses and had various reproduction characteristics. Most of the HIV isolated were rapidly replicating virus variants characterized by an ability to accumulate high levels of virus protein p24 in cultural fluid. Infectivity and reproductive properties of HIV isolates were confirmed in experimental infection by using clarified cultural liquid of mononuclear cells from healthy donors. Phylogenetic analysis of CRF02_AG HIV-1 variants isolated in Novosibirsk region in 2007 - 2010 showed the formation of a separate outbreak in the area caused by emergence of CRF02_AG HIV-1 in human population.
A collection of genetically and biologically characterized CRF02_AG HIV-1 isolates that has not been spreading previously in Russia.
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