Probing electric and magnetic fields with a Moiré deflectometer Lansonneur, P.; Bräunig, P.; Demetrio, A. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
08/2017, Letnik:
862
Journal Article
Recenzirano
A new contact-free approach for measuring simultaneously electric and magnetic field is reported, which considers the use of a low energy ion source, a set of three transmission gratings and a ...position sensitive detector. Recently tested with antiprotons (Aghion et al., 2014) 1 at the CERN Antiproton Decelerator facility, this paper extends the proof of principle of a moiré deflectometer (Oberthaler et al., 1996) 2 for distinguishing electric from magnetic fields and opens the route to precision measurements when one is not limited by the ion source intensity. The apparatus presented, whose resolution is mainly limited by the shot noise is able to measure fields as low as 9mVm−1Hz−1/2 for electric component and 100μGHz−1/2 for the magnetic component. Scaled to 100nm pitch for the gratings, accessible with current state-of-the-art technology 3, the moiré fieldmeter would be able to measure fields as low as 22μVm−1Hz−1/2 and 0.2μGHz−1/2.
ABSTRACT Neospora caninum is the main etiologic agent of neosporosis in domestic animals and its pathogenesis comprises two characteristic phases: acute and chronic. Rodents are used as experimental ...models to mimic acute and chronic bovine neosporosis. In this study, we inoculated a total of 27 female gerbils, with different doses of N. caninum tachyzoites aiming to induce chronic disease. DNA was extracted from different organs of each animal after spontaneous death or euthanasia. Encephalic tissues were submitted to a highly sensitive real time PCR aiming to detect chronically infected animals. All the other samples were submitted to standard PCR. A total of 11 gerbils died due to acute neosporosis, as confirmed by N. caninum DNA detection in organs. 5x103 tachyzoites/mL of N. caninum was the dosage of antigen that can induce chronic infection in gerbils. In the encephalon sections of some animals that showed clinical signs of persistent infection, we found 70% positive for the anterior encephalon section, suggesting this area as preferential for cyst formation. Therefore, we determined the doses of tachyzoites that cause acute or chronic infection and detection of positive tissues, preferably, systemic organs during acute and encephalon in chronic phases.
RESUMO Neospora caninum é o principal agente etiológico da neosporose em animais domésticos, e sua patogênese compreende duas fases características: aguda e crônica. Roedores são usados como modelos experimentais para simular neosporose bovina aguda e crônica. Neste estudo, foi inoculado um total de 27 gerbilos, fêmeas, com diferentes doses de taquizoítos de N. caninum, visando induzir doença crônica. O DNA foi extraído de diferentes órgãos de cada animal após a morte espontânea ou a eutanásia. Os tecidos encefálicos foram submetidos à PCR em tempo real de alta sensibilidade para detecção de animais com infecção crônica. Todas as outras amostras foram submetidas à PCR padrão. Um total de 11 gerbilos morreu devido à neosporose aguda, como confirmado pela detecção de DNA de N. caninum nos órgãos. A dosagem de antígeno que pode induzir infecção crônica foi de 5x103 taquizoítos/mL de N. caninum. Em seções do encéfalo de alguns animais, que apresentaram sinais clínicos de infecção persistente, encontraram-se 70% de positividade para a seção do encéfalo anterior, sugerindo essa área como preferencial para a formação de cisto. Assim, foram determinadas,, em gerbilos, as dosagens de taquizoítos capazes de induzir infecção crônica ou aguda, bem como foram detectados tecidos positivos, preferencialmente, em órgãos sistêmicos, na fase aguda, e no encéfalo, na crônica.
ABSTRACT Free-range chickens may ingest oocysts of T. gondii present in the environment and consequently harbor virulent strains of this parasite in different tissues, without any clinical signs. ...Isolation of T. gondii through bioassays on mice and cats from naturally infected chicken tissues has been described in several countries, demonstrating the importance of free-range chickens in the transmission of this parasite. The aim of this study was the genotypic characterization of T. gondii isolates obtained from naturally infected free-range chickens in a rural area of the state of Rio Grande do Sul, Brazil. Brain and heart tissue from 12 chickens seropositive for T. gondii were processed using peptic digestion technique for parasite isolation. From 12 samples subjected to mouse bioassay, nine isolates were obtained. RFLP-PCR genotypic characterization was performed using 11 genetic markers: SAG1, 5'-3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. Genetic characterization of the isolates revealed the presence of five atypical genotypes according to ToxoDB (# 11, # 55, # 64, # 140 and # 163). Our results showed a wide genetic diversity of T. gondii in free-range chickens in this region.
RESUMO Galinhas criadas ao ar livre podem ingerir oocistos de T. gondii presentes no ambiente e, com isso, albergar cepas virulentas desse parasita em diferentes tecidos, sem sinais clínicos. O isolamento de T. gondii por meio de bioensaios em camundongos e gatos, a partir de tecidos de galinhas naturalmente infectadas, tem sido descrito em vários países. Isso demonstra a importância das galinhas caipiras na epidemiologia desse parasita. O objetivo deste trabalho foi caracterizar genotipicamente isolados de T. gondii obtidos de galinhas caipiras naturalmente infectadas em uma área rural do município de Santa Maria, estado do Rio Grande do Sul, Brasil. Fragmentos de cérebro e de coração, de 12 galinhas soropositivas para T. gondii, foram processados pela técnica de digestão péptica para isolamento do parasita. Das 12 amostras submetidas a bioensaio com camundongos, nove isolados foram obtidos. A caracterização genotípica por RFLP-PCR foi realizada utilizando-se 11 marcadores genéticos: SAG1, 5'-3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 e Apico e revelou a presença de cinco genótipos atípicos de acordo com o ToxoDB (# 11, # 55, # 64, # 140 e # 163). Os resultados mostraram uma ampla diversidade genética de T. gondii em galinhas caipiras nessa região.
ABSTRACT The adipose tissue is a reliable source of Mesenchymal stem cells (MSCs) showing a higher plasticity and transdifferentiation potential into multilineage cells. In the present study, adipose ...tissue-derived mesenchymal stem cells (AT-MSCs) were isolated from mice omentum and epididymis fat depots. The AT-MSCs were initially compared based on stem cell surface markers and on the mesodermal trilineage differentiation potential. Additionally, AT-MSCs, from both sources, were cultured with differentiation media containing retinoic acid (RA) and/or testicular cell-conditioned medium (TCC). The AT-MSCs expressed mesenchymal surface markers and differentiated into adipogenic, chondrogenic and osteogenic lineages. Only omentum-derived AT-MSCs expressed one important gene marker related to male germ cell lineages, after the differentiation treatment with RA. These findings reaffirm the importance of adipose tissue as a source of multipotent stromal-stem cells, as well as, MSCs source regarding differentiation purpose.
RESUMO O tecido adiposo é uma fonte apropriada de células-tronco mesenquimais (MSCs), as quais demonstram ampla plasticidade com capacidade de transdiferenciar em diversas linhagens. No presente estudo, as células-tronco mesenquimais derivadas do tecido adiposo (AT-MSC) foram isoladas de tecido adiposo localizado nas regiões próximas ao omento e testículos de camundongos. Primeiramente, as AT-MSCs foram comparadas com base na expressão de marcadores antigênicos de superfície e no potencial de diferenciação nas três linhagens mesodérmicas. Além disso, AT-MSC, de ambas as fontes, foram cultivadas com meio de diferenciação contendo ácido retinóico (RA) e / ou meio condicionado testicular (TCC). As AT-MSCs expressaram marcadores de superfície mesenquimais e diferenciaram nas linhagens adipogênica, condrogênica e osteogênica. Após o tratamento com RA, somente as AT-MSCs isoladas do tecido adiposo depositado na região do omento expressaram um único importante marcador relacionado às células da linhagem germinativa masculina. Estes resultados reafirmam a importância do tecido adiposo como fonte de células-tronco estromais-multipotentes, bem como, uma fonte de MSCs para estudos de diferenciação.
The planthopper insect Issus produces one of the fastest and most powerful jumps of any insect. The jump is powered by large muscles that are found in its thorax and that, in other insects, ...contribute to both flying and walking movements. These muscles were therefore analysed by transmission electron microscopy to determine whether they have the properties of fast-acting muscle used in flying or those of more slowly acting muscle used in walking. The muscle fibres are arranged in a parallel bundle that inserts onto an umbrella-shaped tendon. The individual fibres have a diameter of about 70 μm and are subdivided into myofibrils a few micrometres in diameter. No variation in ultrastructure was observed in various fibres taken from different parts of the muscle. The sarcomeres are about 15 μm long and the A bands about 10 μm long. The Z lines are poorly aligned within a myofibril. Mitochondrial profiles are sparse and are close to the Z lines. Each thick filament is surrounded by 10–12 thin filaments and the registration of these arrays of filaments is irregular. Synaptic boutons from the two excitatory motor neurons to the muscle fibres are characterised by accumulations of ~60 translucent 40-nm-diameter vesicle profiles per section, corresponding to an estimated 220 vesicles, within a 0.5-μm hemisphere at a presynaptic density. All ultrastructural features conform to those of slow muscle and thus suggest that the muscle is capable of slow sustained contractions in keeping with its known actions during jumping. A fast and powerful movement is thus generated by a slow muscle.
RESUMO O objetivo do presente trabalho foi determinar a presença de anticorpos para Leishmania infantum, Neospora caninum e Toxoplasma gondii, por meio da reação de imunofluorescência indireta ...(RIFI), em cães (n=78) provenientes da região central do Rio Grande do Sul, necropsiados no Hospital Veterinário da Universidade Federal de Santa Maria (UFSM), bem como avaliar os dados epidemiológicos, sazonais e anátomo-histopatológicos. Do total de animais avaliados, 67,9% (53/78) apresentaram soropositividade para ao menos um agente. A ocorrência de anticorpos para L. infantum, N. caninum e T. gondii foi de 33,3 (26/78), 37,1 (29/78) e 43,5% (34/78), respectivamente. Detectaram-se monoinfecções em 9,4% (5/53) para L. infantum, 18,8% (10/53) para N. caninum e 20,7% (11/53) para T. gondii. As coinfecções foram observadas em 27/53 (50,9%) dos animais. As infecções ocorreram independentemente de idade, sexo, procedência ou raça (P>0,05). Não se verificaram lesões anatomo-histopatológicas relacionadas aos agentes pesquisados, caracterizando-os como animais assintomáticos. Os resultados confirmaram a exposição de cães a esses protozoários na região central do RS e, em especial, demonstraram a circulação do agente causador da leishmaniose em uma área considerada indene para a enfermidade.
Oral and long-acting risperidone has been shown to be effective for acute and
maintenance treatment of patients with schizoaffective disorders. The present
analysis investigated the efficacy and ...tolerability of direct transition from other
antipsychotics to risperidone long-acting injectable in patients with
schizoaffective disorder.Patients aged ≥ 18 years with schizoaffective disorder (DSM-IV), who required
a change of medication, received risperidone long-acting injectable 25mg (increased
to 37.5 or 50mg, if necessary) every 2 weeks for 6 months.The analysis included 249 patients (47% male; mean age 43 years), of whom 74%
completed the 6-month study. Mean scores for the total Positive and Negative
Syndrome Scale (PANSS) and all three subscales were significantly reduced from
baseline to week 4 (p < 0.001), with further improvements until
treatment endpoint. Significant improvements from baseline to endpoint were seen in
the mood symptom domains of anxiety/depression (10.4±4.1 vs
8.7±3.9) and uncontrolled hostility/excitement (7.6±3.6 vs
6.9±3.8). Mean Global Assessment of Function (GAF) score improved
significantly from 59.4±15.6 at baseline to 66.4±17.7
(p < 0.001) at endpoint. Of 87 patients hospitalized at baseline,
67% were discharged at endpoint. Both quality of life (SF-36) and satisfaction with
treatment were improved significantly at endpoint. Total ESRS scores fell
progressively throughout the study, and the reduction was already statistically
significant (p < 0.001) at 4 weeks. Small but statistically significant
(p < 0.001) mean shifts of 1.8% were seen in body weight and Body
Mass Index (BMI).Patients with schizoaffective disorder derived several benefits from a change to
risperidone long-acting injectable, including reductions in psychiatric symptoms
(particularly the mood symptom domains) and a reduction in the severity of
drug-induced neurological movement disorders.
The principal aim of the AEgIS experiment at CERN is to measure the acceleration of antihydrogen due to Earth's gravitational field. This would be a test of the Weak Equivalence Principle, which ...states that all bodies fall with the same acceleration independently of their mass and composition. The effect of Earth's gravitational field on antimatter will be determined by measuring the deflection of the path of the antihydrogen from a straight line. The position of the antihydrogen will be found by detecting its annihilation on the surface of a silicon detector. The gravitational measurement in AEgIS will be performed with a gravity module, which includes the silicon detector, an emulsion detector and a scintillating fibre time-of-flight detector. As the experiment attempts to determine the gravitational acceleration with a precision of 1%, a position resolution better than 10 μm is required. Here we present the results of a study of antiproton annihilations in a 3D silicon pixel sensor and compare the results with a previous study using a monolithic active pixel sensor. This work is part of a larger study on different silicon sensor technologies needed for the development of a silicon position detector for the AEgIS experiment. The 3D detector together with its readout electronics have been originally designed for the ATLAS detector at the LHC. The direct annihilation of low energy antiprotons ( ~ 100 keV) takes place in the first few μm of the silicon sensor and we show that the charged products of the annihilation can be detected with the same sensor. The present study also aims to understand the signature of an antiproton annihilation event in segmented silicon detectors and compares it with a GEANT4 simulation model. These results will be used to determine the geometrical and process parameters to be adopted by the silicon annihilation detector to be installed in AEgIS.
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