Small polymer particles with a diameter of less than 5 mm called microplastics find their way into the environment from polymer debris and industrial production. Therefore a method is needed to ...identify and quantify microplastics in various environmental samples to generate reliable concentration values. Such concentration values, i.e. quantitative results, are necessary for an assessment of microplastic in environmental media. This was achieved by thermal extraction in thermogravimetric analysis (TGA), connected to a solid-phase adsorber. These adsorbers were subsequently analysed by thermal desorption gas chromatography mass spectrometry (TDS-GC-MS). In comparison to other chromatographic methods, like pyrolyse gas chromatography mass spectrometry (Py-GC-MS), the relatively high sample masses in TGA (about 200 times higher than used in Py-GC-MS) analysed here enable the measurement of complex matrices that are not homogenous on a small scale. Through the characteristic decomposition products known for every kind of polymer it is possible to identify and even to quantify polymer particles in various matrices. Polyethylene (PE), one of the most important representatives for microplastics, was chosen as an example for identification and quantification.
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•Using a thermal decomposition method for analysing PE microplastics in environmental matrices in one step.•Direct measuring without a pre selection.•Identification and quantification of polymers in environmental samples.
Bovine mastitis is an important health and cost factor in the milk industry. To elucidate whether isolated perfused bovine udders can be used to study early inflammatory events of mastitis, 1 mg of ...lipopolysaccharide (LPS) was instilled into quarters of 10 isolated perfused bovine udders. Three hours and 6 h after LPS instillation, tissue samples were taken from the gland cistern and base of the udder, subsequently stored in RNAlater and processed for the determination of inflammation-dependent gene regulation by real-time RT-qPCR. Gene expression analysis was performed using delta-delta Ct method. To translate mRNA results to protein, IL-1ß and IL-6 were determined in tissue homogenate by ELISA.
The instillation of 1 mg LPS lead to an increased expression of pro-inflammatory cytokines and chemokines like TNF-α, CCL20, CXCL8 as well as of IL-1 ß, IL-6 and IL-10, lingual antimicrobial peptide (LAP) and S100A9. However, the degree of elevation differed slightly between gland cistern and udder base and markedly between 3 and 6 h after instillation, with a distinct increase in mediator expression after 6 h. IL-1β protein increased in a time-dependent manner, whereas IL-6 was unchanged within 6 h of LPS instillation.
Compared to in vivo studies with instillation of LPS into udders of living cows, a similar inflammation-dependent gene regulation profile can be mimicked in the isolated perfused bovine udder, indicating a supplementation of animal experiments.
Adoptive transfer of autologous tumor-specific lymphocytes represents a viable treatment method for patients with advanced malignancies. Here, we report a patient's case with metastatic ...hormone-refractory New York esophageal squamous cell carcinoma 1 (NY-ESO-1) expressing prostate cancer treated with in vitro expanded tumor-infiltrating lymphocytes (TILs) in conjunction with IL-2 and immune-checkpoint blockade. Complete and durable tumor remission was observed after three TIL infusions consisting of 1.4×10
, 2.0×10
, and 8.0×10
T cells, respectively, lasting now for more than 3.5 years. Immunological correlates to the clinical development were the decrease of tumor-driven NY-ESO-1 serum antibody and the drop of prostate-specific antigen to <0.01 µg/L. TILs were reactive against cancer-testis antigen NY-ESO-1, individual tumor mutational proteins (eg, PRPF8, TRPS1), and the androgen receptor splice variant 12.
The synthetic oligodeoxynucleotide CpG 7909, which contains unmethylated cytosine/guanine (CpG) motifs, has potent immunostimulatory effects when coadministered with NY-ESO-1 peptides or recombinant ...NY-ESO-1 protein, resulting in an enhanced cellular and humoral immune response against the vaccine antigen. In this study, we report the development of anti-CpG-ODN antibodies in 21 of 37 patients who received CpG 7909 either alone or as a vaccine adjuvant. Specific anti-CpG immunoglobulin G (IgG) antibody titers ranged from 1:400 to 1:100,000. The anti-CpG antibodies cross-reacted with other synthetic CpG-ODNs but not with the DNA of mixed bacterial vaccine and were shown to be phosphorothioate backbone specific. Vaccine-related severe side effects observed in some patients were most likely not related to the development of anti-CpG antibodies. In addition, anti-CpG antibodies did not have negative effects on the vaccine immune response. These results show that anti-CpG antibodies develop in humans against short unmethylated CpG dinucleotide sequences after administration of CpG 7909. Our data therefore substantiate the potency of CpG 7909 to directly stimulate human B-cells and suggest that anti-CpG antibody monitoring should be a part of ongoing and planned clinical trials with CpG-ODNs.
Modern media report news remarkably fast, often before the information is confirmed. This general tendency is even more pronounced in times of an increasing demand for information, such as during ...pressing natural phenomena or the pandemic spreading of diseases. Yet, even if early reports correctly identify their content as speculative (rather than factual), recipients may not adequately consider the preliminary nature of such information. Theories on language processing suggest that understanding a speculation requires its reconstruction as a factual assertion first-which can later be erroneously remembered. This would lead to a bias to remember and treat speculations as if they were factual, rather than falling for the reverse mistake. In six experiments, however, we demonstrate the opposite pattern. Participants read news headlines with explanations for distinct events either in form of a fact or a speculation (as still being investigated). Both kinds of framings increased participants' belief in the correctness of the respective explanations to an equal extent (relative to receiving no explanation). Importantly, however, this effect was not mainly driven by a neglect of uncertainty cues (as present in speculations). In contrast, our memory experiments (recognition and cued recall) revealed a reverse distortion: a bias to falsely remember and treat a presented "fact" as if it were merely speculative. Based on these surprising results, we outline new theoretical accounts on the processing of (un)certainty cues which incorporate their broader context. Particularly, we propose that facts in the news might be remembered differently once they are presented among speculations.
Modern media enable rapid reporting that does not refer to facts alone but is often interspersed with unconfirmed speculations. Whereas previous research has concentrated primarily on how unconfirmed ...contents might propagate, potential memory effects of reporting confirmed facts among speculations have so far been widely disregarded. Across four experiments, we show that the presence of speculative news (indexed by uncertainty cues such as "might") can reduce the remembered certainty of unrelated facts. The participants read headlines with exclusively speculative news, exclusively factual news, or a mixture of both. Our results indicate that uncertainty cues spread onto one's recollection of unrelated facts after having read a mixture of facts and speculations. This tendency persisted when both types of news were presented sequentially (e.g., factual news first), suggesting that the presence of speculative news does not specifically affect encoding-but can overshadow memories of facts in retrospect. Further, the tendency to misremember facts as speculations emerged even when the proportion of speculations among factual news was low (6/24 headlines) but increased linearly with the number of speculations intermingled. Given the widespread dissemination of speculative news, this bias poses a challenge in effectively getting confirmed information across to readers.
Public Significance Statement
This study demonstrates that intermingling speculative, unconfirmed news (e.g., "X might have caused Y") can lead readers to misremember contentwise unrelated factual news (e.g., "A caused B") as unconfirmed information. Given that pieces of speculative news are widely spread, this bias poses a challenge in communicating confirmed contents (facts) as such.
Trust during retail encounters: A touchy proposition Orth, Ulrich R.; Bouzdine-Chameeva, Tatiana; Brand, Kathrin
Journal of retailing,
September 2013, 2013-9-00, 20130901, Letnik:
89, Številka:
3
Journal Article
Recenzirano
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► We examine how a salesperson's touch affects customers’ product evaluation. ► We focus on trust as an important mediator of the touch-evaluation relation. ► Individual need for ...touch and culture-specific touching behavior are moderators. ► Data are obtained from eighteen professionals and 120 consumers in two cultures. ► The findings support decisions on how to increase trust and product evaluation.
Adopting an interpersonal communication perspective, this study examines the propositions that a salesperson's touch increases trust, which increases product evaluations and purchase intention. These relationships are evaluated in a contact and non-contact culture, with need for touch (NFT) examined as an additional moderator. An exploratory series of in-depth interviews provides an initial understanding of these relationships, followed by a 2 (touch/no touch condition)×2 (consumers in France/Germany) experiment with wine serving as the example category. The findings indicate that touch does not uniformly instill trust in customers. Instead a salesperson's touch relates to greater trust only when consumers have an inherent NFT or when they are from a culture where personal touching behavior is less prevalent. Trust, in turn, relates positively to evaluations of product attractiveness, quality, and to purchase intention.
Mixed bacterial vaccine (MBV, Coley's toxins) is a historical, vaguely defined preparation of heat-inactivated Streptococcus pyogenes and Serratia marcescens used as nonspecific immunotherapy in the ...treatment of cancer. The mechanism of action is suspected to have an immunologic basis, yet it is poorly defined up to now. We developed a new, biochemically well defined and current good manufacturing practice-compliant MBV preparation, which has been investigated in patients with NY-ESO-1 expressing cancers.
Patients received MBV subcutaneously at a starting dose of 250 EU (endotoxin units) twice a week. The MBV dose was escalated in each patient until a body temperature of 38°C to 39.5°C was induced or up to the maximum dose of 547.000 EU. Changes in serum cytokine levels were determined and immune responses to NY-ESO-1 were evaluated. Tumor response was assessed according to RECIST.
Twelve patients were enrolled and 11 of them developed fever after the administration of MBV. Ten of 12 patients showed a consistent increase in serum IL-6 levels with the highest levels coinciding with the highest body temperature. A subgroup of patients showed increasing levels of TNF-α, IFN-γ, and IL1-β. A patient with metastatic bladder cancer showed a partial tumor response strongly correlated with MBV-induced fever and highly elevated levels of several cytokines.
MBV at fever-inducing dose levels can lead to a massive induction of immunoregulatory cytokines that may be involved in inducing tumor regressions. We propose to further explore the role of MBV as a potent immune modulator at higher dose levels and in conjunction with antigen-specific cancer vaccines.
NY-ESO-1, one of the most immunogenic tumor antigens, is expressed in 15% to 25% of metastatic prostate cancers. The immunological and clinical effects of vaccination with recombinant NY-ESO-1 ...protein combined with CpG as adjuvant were evaluated.
In a phase I clinical study, patients with advanced prostate cancer were vaccinated with recombinant NY-ESO-1 protein (100 μg) mixed with CpG 7909 (2.5 mg) every 3 weeks intradermally for 4 doses. Objectives of the study were the safety of the vaccine and changes of specific humoral and cellular immunological responses to NY-ESO-1 in relation to detectable NY-ESO-1 expression in the individual tumor.
All 12 baseline sero-negative patients developed high-titer NY-ESO-1 antibody responses. B-cell epitope mapping identified NY-ESO-1 p91-110 to be recognized most frequently by vaccine-induced antibodies. Two patients developed significant antibody titers against the adjuvant CpG. NY-ESO-1-specific CD4+ and/or CD8+ T-cell responses were induced in 9 patients (69%). Five of these 9 patients did not express NY-ESO-1 in the autologous tumor. Postvaccine CD8+ T-cell clones recognized and lyzed HLA-matched tumor cell lines in an antigen-specific manner.
Our data provide clear evidence for the capacity of NY-ESO-1 protein/CpG vaccine to induce integrated antigen-specific immune responses in vivo and to efficiently prime CD8+ T-cell responses in NY-ESO-1 antigen-negative patients. Our results may also support further clinical vaccination protocols with NY-ESO-1 protein not only focused on the treatment of existing cancer, but also to prevent further development of NY-ESO-1 positive cancers in vivo.
In scholarly and political debates on transformation towards sustainability or along social-ecological lines, employees and trade unions are hardly ever mentioned. The latter still largely represent ...a perspective that could be summarized as “jobs versus environment” and formulate their environmental strategies within the confines of ecological modernization, if indeed at all -- i.e. they do not question the orientation towards growth, capitalist power and production relations or a destructive societal nature relations. It was strong trade unions who won historical achievements for large parts of the population, but these achievements simultaneously deepened the problematic aspects of a mode of production and living which we call the “imperial mode of living”. This antinomy needs to be considered and dealt with politically. This paper examines the results of a multi-year empirical project on the views of Austrian trade unions on social-ecological transformation. It shows that, while there are already some important initiatives, these do not address the main challenge, i.e. the imperial mode of living. However, some entry points for a possible politicization of unsustainable production and consumption emerge from our research, along with a view of the role of trade unions in this politicization. One approach is to overcome the narrow equation of well-being with capitalist economic growth and to replace it with a more comprehensive understanding of well-being and quality of life.
•The literature on social-ecological transformation insufficiently explores the role of employees and trade unions.•Research results on “social-ecological entry points” for trade unions are presented.•Trade unions formulate environmental policies in the confines of ecological modernization.•The imperial mode of living is based on unsustainable consumption and production patterns.•This mode of (production and) living needs to be addressed by trade unions.
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