Pathology in Practice Breshears, Melanie A.; Meinkoth, James H.; Stern, Adam W. ...
Journal of the American Veterinary Medical Association,
01/2011, Letnik:
238, Številka:
2
Journal Article
Pathology in Practice Cramer, Sarah D.; Breshears, Melanie A.; Qualls, Heath J.
Journal of the American Veterinary Medical Association,
03/2011, Letnik:
238, Številka:
5
Journal Article
Abstract only
MicroRNAs (miRNAs) are small RNAs with a length of ~21‐24 nucleotides. They are endogenous in origin and regulate the expression of proteins post‐transcriptionally. miRNA expression ...profile in a disease provides valuable insights into the pathogenesis of that disease and possible therapeutic strategies. Bronchopulmonary dysplasia (BPD) is the chronic lung disease manifested in premature infants and has multifactorial etiology ranging from hyperoxia exposure to premature lung to neonatal therapeutics. Advancement in treating premature infants using new management and therapeutic strategies has changed the course of BPD pathogenesis. In this study we used a 'new' BPD model where the primary lesions have shifted from chronic inflammatory responses, as seen in 'old' BPD models, to actual dysplasia. Infant rats were exposed to 95% oxygen from postnatal day 3 to day 14. The time point selected showed changes in the lung that mimicked the new BPD pathology. Small RNA fraction was isolated and miRNA microarray was performed on BPD and control samples. We found that five miRNAs, miR‐342, miR‐335, miR‐150, miR‐126* and miR‐151* were down‐regulated and three miRNAs, miR‐21, miR‐141 and miR‐34a were up‐regulated in the BPD lung. Most of these miRNAs have target proteins vital to normal lung development and the study thus provide us with new insights into miRNA‐mediated regulation of BPD pathogenesis.
The objective of this study was to determine the effect of season on the histologic and histomorphometric appearance of the normal equine pituitary gland. Pituitary glands were collected at necropsy ...from 121 horses throughout the year. Plasma was also collected from 59 of these horses before euthanasia. Hematoxylin and eosin stained median sagittal sections of each pituitary were evaluated and histologically graded by three pathologists. Histomorphometric analysis was performed on the same slides. Plasma α-melanocyte stimulating hormone was measured by radioimmunoassay in a subset of horses (n = 59). A total of 118 pituitary glands were included in the study after exclusions were made on the basis of the presence of pars intermedia (PI) adenomas (>5 mm). There was a positive correlation between PI hormone concentration (α-melanocyte stimulating hormone) and PI area. Pituitary gland measurements and grades from samples collected in the fall were compared with those collected in the nonfall months using
t-test. The PI area, total pituitary area, and PI/total pituitary ratio were significantly greater in the fall compared with nonfall months (
P < .0001,
P < .01,
P < .0001, respectively). Pituitary grades were also higher in the fall compared with nonfall months (
P < .001). There was no seasonal difference in pars distalis or pars nervosa area. The results of this study show that the normal equine pituitary shows seasonal changes in appearance and size. These changes must be considered when using postmortem histologic evaluations in the diagnosis of pituitary pars intermedia dysfunction or for validation of antemortem diagnostic tests.
Pathology in practice. Renal lymphoma Breshears, Melanie A; Meinkoth, James H; Stern, Adam W ...
Journal of the American Veterinary Medical Association,
2011-Jan-15, 20110115, Letnik:
238, Številka:
2
Journal Article
Honey mesquite toxicosis in a goat Washburn, Kevin E; Breshears, Melanie A; Ritchey, Jerry W ...
Journal of the American Veterinary Medical Association,
06/2002, Letnik:
220, Številka:
12
Journal Article
Recenzirano
Honey mesquite (Prosopis glandulosa) is distributed across a large portion of the southwestern United States. Ingestion of young leaves, pods, or beans can cause toxicosis in cattle and goats if they ...comprise a substantial portion of their diet. Goats, as browsers, are most likely to develolp mesquite toxicosis. Sheep appear to be more resistant to the plant's toxic effects. Consistent clinical signs include weight loss, ptyalism, mandibular tremors, tongue protrusion, and dysphagia. Diagnosis of mesquite toxicosis is largely made on the basis of history and clinical signs with exclusion of appropriate differentials. Laboratory findings are nonspecific but may reveal a mild anemia and hypoglycemia. Postmortem findings suggestive of mesquite toxicosis are limited to fine vacuolation of neurons in the trigeminal motor nucleus. Treatment consists of an alternative diet and supportive care. The disease is treatable in cattle and sheep but has a high case fatality rate in goats.
A 5-month-old mixed-breed filly presented with diarrhea due to Salmonella typhimurium infection and subsequently developed pneumonia in addition to ischemic necrosis of distal limbs. Pulmonary ...lesions were characterized by numerous discrete, disseminated pyogranulomas with intralesional fungal hyphae. The morphologic characteristics of fungal hyphae were consistent with Aspergillus spp., and large numbers of A fumigatus were isolated from lung tissue via fungal culture. Lesions in all 4 limbs were similar in distribution, duration, and severity and were characterized by coagulation necrosis accompanied by occasional thrombi in small vessels without evidence of thrombosis of larger limb arteries. Thus, limb lesions are consistent with symmetrical peripheral gangrene, a potential complication of sepsis.
What Is Your Diagnosis? Haak, Carol E.; Breshears, Melanie A.; Lackner, Petra A.
Journal of the American Veterinary Medical Association,
09/2007, Letnik:
231, Številka:
6
Journal Article
What is your diagnosis? Retrobulbar neoplasia Haak, Carol E; Breshears, Melanie A; Lackner, Petra A
Journal of the American Veterinary Medical Association,
2007-Sep-15, 20070915, Letnik:
231, Številka:
6
Journal Article
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