Stream macroinvertebrate assemblages are shaped by natural and human‐related factors that operate through complex hierarchical pathways. Quantifying these relationships can provide additional ...insights into stream ecological assessment. We applied a structural equation modeling framework to evaluate hypothesized pathways by which watershed, riparian, and in‐stream factors affect benthic macroinvertebrate condition in the Western Mountains (WMT) and Xeric (XER) ecoregions in the United States. We developed a conceptual model grounded in theory, empirical evidence, and expert opinion to evaluate the following hypotheses: (1) macroinvertebrate assemblages are primarily driven by proximal, in‐stream factors (e.g., water quality and physical habitat); (2) anthropogenic land uses affect macroinvertebrates indirectly by altering in‐stream characteristics; and (3) riparian vegetation cover attenuates land use effects. We tested our model separately on three measures of benthic macroinvertebrate assemblage condition: ratio of observed‐to‐expected taxonomic richness (O/E); a multimetric index (MMI); and richness of Ephemeroptera, Plecoptera, and Trichoptera taxa (EPT). In the WMT, site‐level riparian cover, in‐stream physical habitat (relative bed stability), and water chemistry (total nitrogen) were the top three predictors of macroinvertebrate assemblages, each having over two times the magnitude of effect on macroinvertebrates compared with watershed‐level predictors. In the arid XER, annual precipitation and stream flow characteristics were top predictors of macroinvertebrate assemblages and had similar magnitudes of effect as in‐stream water chemistry. Path analyses revealed that land use activities in the watershed and at the stream site degraded macroinvertebrate assemblages indirectly by altering relative bed stability, water quality, and riparian cover/complexity. Increased riparian cover was associated with greater macroinvertebrate condition by reducing land use impacts on stream flow, streambed substrate, and water quality, but the pathways differed among ecoregions. In the WMT, site‐level riparian cover affected macroinvertebrate assemblages partly through indirect pathways associated with greater streambed stability and reduced total nitrogen concentrations. In contrast, in the XER, watershed‐level riparian cover affected macroinvertebrate assemblages through greater specific stream power. Identifying the relative effects of and pathways by which natural and anthropogenic factors affect macroinvertebrates can serve as a framework for prioritizing management and conservation efforts.
Nitrogen fertilizer applications are common land use management tools, but details on physiological responses to these applications are often lacking, particularly for longterm . responses over ...decades of forest management. We used tree ring growth patterns and stable isotopes to understand long-term physiological responses to fertilization using a controlled fertilization experiment begun in 1964 in Washington State (USA), in which three levels of nitrogen fertilizer were applied: 157, 314, and 471 kg/ha. Basal area increment (BAI) increased more than fourfold in the highest treatment to twofold in the lowest, and a significant increase in BAI was observed for 20 years. Latewood Δ¹³C sharply decreased by 1.4‰ after fertilization and was significantly lower than controls for four years, but no differences existed between fertilization levels, and the effect disappeared after four years, indicating that intrinsic water use efficiency ($A/g_s $) increased in response to fertilization. Earlywood Δ¹³C showed similar trends but was more variable. Latewood δ¹ɸO increased significantly above controls by ~ 2‰ in all treatments, but the duration differed with treatment level, with the effect being longer for higher levels of fertilization and lasting as long as nine years after fertilization. Because source water and relative humidity were the same between experimental plots, we interpreted the δ¹⁸O increase with treatment as a decrease in leaf-level transpiration. Earlywood δ¹⁸O did not show any treatment effects. Because the Pacific Northwest has a mediterranean climate with dry summers, we speculated that fertilization caused a substantial increase in leaf area, causing the trees to transpire themselves into drought stress during the late summer. We estimate from the δ¹⁸O data that stomatal conductance ($g_s $) was reduced by — 30%. Using the Δ¹³C data to estimate assimilation rates (A), A during the late season was also reduced by 20-30%. If leaf-level A decreased, but BAI increased, we estimated that leaf area on those trees must have increased by fourfold with the highest level of treatment within this stand. This increase in leaf area resulting from fertilization caused a hydraulic imbalance within the trees that lasted as long as nine years after treatment at the highest levels of fertilization.
1. We investigated the potential of cross-scale interactions to affect the outcome of density reduction in a large-scale silvicultural experiment to better understand options for managing forests ...under climate change. 2. We measured tree growth and intrinsic water-use efficiency (iWUE) based on stable carbon isotopes (δ¹³C) to investigate impacts of density reduction across a range of progressively finer spatial scales: site, stand, hillslope position and neighbourhood. In particular, we focused on the influence of treatments beyond the boundaries of treated stands to include impacts on downslope and neighbouring stands across sites varying in soil moisture. 3. Trees at the wet site responded with increased growth when compared with trees at the dry site. Additionally, trees in treated stands at the dry site responded with increased iWUE while trees at the wet site showed no difference in iWUE compared to untreated stands. 4. We hypothesized that water is not the primary limiting factor for growth at our sites, but that density reduction released other resources, such as growing space or nutrients to drive the growth response. At progressively finer spatial scales we found that tree responses were not driven by hillslope location (i.e. downslope of treatment) but to changes in local neighbourhood tree density. 5. Synthesis. This study demonstrated that water can be viewed as an agent to investigate cross-scale interactions as it links processes operating at coarse to finer spatial scales and vice versa. Consequently, management prescriptions such as density reductions to increase resistance and resilience of trees to climate change, specifically to drought, need to consider cross-scale interactions as specific magnitude and mechanisms of growth responses can only be predicted when multiple scales are taken into account.
Tree‐ring characteristics are commonly used to reconstruct climate variables, but divergence from the assumption of a single biophysical control may reduce the accuracy of these reconstructions. ...Here, we present data from bur oaks (Quercus macrocarpa Michx.) sampled within and beyond the current species bioclimatic envelope to identify the primary environmental controls on ring‐width indices (RWIs) and carbon stable isotope discrimination (Δ¹³C) in tree‐ring cellulose. Variation in Δ¹³C and RWI was more strongly related to leaf‐to‐air vapour pressure deficit (VPD) at the centre and western edge of the range compared with the northern and wettest regions. Among regions, Δ¹³C of tree‐ring cellulose was closely predicted by VPD and light responses of canopy‐level Δ¹³C estimated using a model driven by eddy flux and meteorological measurements (R² = 0.96, P = 0.003). RWI and Δ¹³C were positively correlated in the drier regions, while they were negatively correlated in the wettest region. The strength and direction of the correlations scaled with regional VPD or the ratio of precipitation to evapotranspiration. Therefore, the correlation strength between RWI and Δ¹³C may be used to infer past wetness or aridity from paleo wood by determining the degree to which carbon gain and growth have been more limited by moisture or light.
Nitrogen (N) removal along flowpaths to aquatic ecosystems is an important regulating ecosystem service that can help reduce N pollution in the nation's waterways, but can be challenging to measure ...at large spatial scales. Measurements that integrate N processing within watersheds would be particularly useful for assessing the magnitude of this vital service. Because most N removal processes cause isotopic fractionation, δ15N from basal food-chain organisms in aquatic ecosystems can provide information on both N sources and the degree of watershed N processing. As part of EPA's National Aquatic Resource Surveys (NARS), we measured δ15N of Chironomidae collected from over 2000 lakes, rivers and streams across the continental USA. Using information on N inputs to watersheds and summer total N concentrations (TN) in the water column, we assessed where elevated chironomid δ15N would indicate N removal rather than possible enriched sources of N. Chironomid δ15N values ranged from −4 to +20‰, and were higher in rivers and streams than in lakes, indicating that N in rivers and streams underwent more processing and cycling that preferentially removes 14N than N in lakes. Chironomid δ15N increased with watershed size, N inputs, and water chemical components, and decreased as precipitation increased. In rivers and streams with high watershed N inputs, we found lower TN in streams with higher chironomid δ15N values, suggesting high rates of gaseous N loss such as denitrification. At low watershed N inputs, the pattern reversed; streams with elevated chironomid δ15N had higher TN than streams with lower chironomid δ15N, possibly indicating unknown sources elevated in δ15N such as legacy N, or waste from animals or humans. Chironomid δ15N values can be a valuable tool to assess integrated watershed-level N sources, input rates, and processing for water quality monitoring and assessment at large scales.
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•The δ15N of chironomids integrates nitrogen sources and processes across watersheds.•We measured chironomid δ15N in over 2000 locations across the USA.•With high N inputs, increasing chironomid δ15N indicated higher N removal processes.•With low N inputs, increasing chironomid δ15N indicated N sources enriched in 15N.•δ15N of chironomids helps inform national monitoring programs about N as a stressor.
The heart of forensic science is application of the scientific method and analytical approaches to answer questions central to solving a crime: Who, What, When, Where, and How. Forensic practitioners ...use fundamentals of chemistry and physics to examine evidence and infer its origin. In this regard, ecological researchers have had a significant impact on forensic science through the development and application of a specialized measurement technique—isotope analysis—for examining evidence. Here, we review the utility of isotope analysis in forensic settings from an ecological perspective, concentrating on work from the Americas completed within the last three decades. Our primary focus is on combining plant and animal physiological models with isotope analyses for source inference. Examples of the forensic application of isotopes—including stable isotopes, radiogenic isotopes, and radioisotopes—span from cotton used in counterfeit bills to anthrax shipped through the U.S. Postal Service and from beer adulterated with cheap adjuncts to human remains discovered in shallow graves. Recent methodological developments and the generation of isotope landscapes, or isoscapes, for data interpretation promise that isotope analysis will be a useful tool in ecological and forensic studies for decades to come.
In mountainous river basins of the Pacific Northwest, climate models predict that winter warming will result in increased precipitation falling as rain and decreased snowpack. A detailed ...understanding of the spatial and temporal dynamics of water sources across river networks will help illuminate climate change impacts on river flow regimes. Because the stable isotopic composition of precipitation varies geographically, variation in surface water isotope ratios indicates the volume‐weighted integration of upstream source water. We measured the stable isotope ratios of surface water samples collected in the Snoqualmie River basin in western Washington over June and September 2017 and the 2018 water year. We used ordinary least squares regression and geostatistical Spatial Stream Network models to relate surface water isotope ratios to mean watershed elevation (MWE) across seasons. Geologic and discharge data was integrated with water isotopes to create a conceptual model of streamflow generation for the Snoqualmie River. We found that surface water stable isotope ratios were lowest in the spring and highest in the dry, Mediterranean summer, but related strongly to MWE throughout the year. Low isotope ratios in spring reflect the input of snowmelt into high elevation tributaries. High summer isotope ratios suggest that groundwater is sourced from low elevation areas and recharged by winter precipitation. Overall, our results suggest that baseflow in the Snoqualmie River may be relatively resilient to predicted warming and subsequent changes to snowpack in the Pacific Northwest.
Water sources in a mountain river were evaluated using stable isotopes of water. Stable isotope ratios varied strongly with mean watershed elevation. Depleted isotope in spring reflect snowmelt moving quickly through the watershed, and enriched summer isotope ratios suggest that groundwater is sourced from low elevation areas and recharged by winter precipitation. Our results suggest that baseflow in the Snoqualmie River may be relatively resilient to predicted warming and subsequent changes to snowpack in the Pacific Northwest.
A substantial fraction of nitrogen (N) fertilizer applied in agricultural systems is not incorporated into crops and moves below the rooting zone as nitrate (NO3−). Understanding mechanisms for soil ...N retention below the rooting zone and leaching to groundwater is essential for our ability to track the fate of added N. We used dual stable isotopes of nitrate (δ15N–NO3− and δ18O–NO3−) and water (δ18O–H2O and δ2H–H2O) to understand the mechanisms driving nitrate leaching at three depths (0.8, 1.5, and 3.0 m) of an irrigated corn field sampled every 2 weeks from 2016 to 2020 in the southern Willamette Valley, Oregon, USA. Distinct periods of high nitrate concentrations with lower δ15N–NO3− values indicated that a portion of that nitrate was from recent fertilizer applications. We used a mixing model to quantify nitrate fluxes associated with recently added fertilizer N versus older, legacy soil N during these “fertilizer signal periods.” Nitrate leached below 3.0 m in these periods made up a larger proportion of the total N leached at that depth (∼52%) versus the two shallower depths (∼13%–16%), indicating preferential movement of recently applied fertilizer N through the deep soil into groundwater. Further, N associated with recent fertilizer additions leached more easily when compared to remobilized legacy N. A high volume of fall and winter precipitation may push residual fertilizer N to depth, potentially posing a larger threat to groundwater than legacy N. Optimizing fertilizer N additions could minimize fertilizer losses and reduce nitrate leaching to groundwater.
Core Ideas
A total of 11% (22.7 kg N·ha−1·year−1) of recently applied fertilizer was leached below 3 m with the onset of fall rain.
Processed legacy nitrogen (N) comprised up to 18% (32.8 kg N·ha−1·year−1) of nitrate lost to leaching.
Denitrification was not an important process contributing to N removal.
Residual fertilizer N posed a greater immediate threat to groundwater than soil legacy N.
N sources and potential processing information can link soil surface practices with nitrate leaching.
Determining how water sources for rivers vary over time can greatly enhance our understanding and management of land use and climate change impacts on rivers. Because the stable isotope composition ...of precipitation can vary geographically, variation in the stable isotope composition of river water may be able to identify source water dynamics. We monitored the stable isotope values (δ
18
O and δ
2
H) of river and stream water within the southern Willamette River Basin in western Oregon over two years. Within this basin, eighty-four percent of the isotopic variation in small tributary streams was explained by the mean elevation of the catchments, whereas seasonal variation was minimal. However, water within the Willamette River had distinct isotopic seasonal patterns that likely occurred because of changes in the mean elevation of source water for the river. River isotopic values were lowest during summer low flow and highest during February/March when snow accumulated in the mountains. We estimated that the mean elevation of the source water for the Willamette River shifted over 700 m, seasonally. During winter when rain occurred in the valley and snow accumulated in the mountains, the river reflected a mixture of low mountains and valley bottom precipitation. During the dry Mediterranean summer, 60-80% of the river water came from the snow zone above 1200 m, which is only 12% of the land area and accounts for 15.6% of the annual precipitation within the Willamette Basin. This high elevation area contains the High Cascades geological region with highly permeable bedrock that sustains late-summer baseflow compared to the Western Cascades with low permeable bedrock. Reliance on high-elevation water during summer low flow highlights the vulnerability of this system to influences of a warming climate, where snowpacks in the Cascade Mountains are predicted to decrease in the future.