Una drammatica testimonianza di un sopravvissuto alla deportazione nel campo di concentramento di Auschwitz-Birkenau, che solo per un caso non trovò la morte, dopo essere stato rinchiuso in una ...camera a gas per un giorno. Ledizioni ripubblica questa testimonianza, per non dimenticare. Con una prefazione di Marcello Flores.
A dramatic testimony of a deportation survivor in the Auschwitz-Birkenau concentration camp, who only died one case after being locked in a gas chamber for a day. Editions republish this testimony, ...not to forget. With a preface by Marcello Flores.
This study aimed to analyze the chemical-physical properties, including pH, volumetric change, radiopacity, and apatite-forming ability in simulated body fluid, of a new tricalcium silicate material ...(MTA Flow; Ultradent Products Inc, South Jordan, UT).
MTA Flow was tested in comparison with MTA Angelus (Angelus, Londrina, PR, Brazil). The pH of soaking water was tested up to 168 hours in deionized water. In the solubility test, the root-end fillings of 20 acrylic teeth were scanned twice by micro–computed tomographic imaging before and after immersion in ultrapure water for 168 hours. In addition, using an aluminum step wedge, the radiopacity of each material was evaluated as recommended by international standards. The mean gray values of the test materials were measured using ImageJ software (National Institutes of Health, Bethesda, MD). The morphologic and chemical analyses of the material surface were performed using scanning electron microscopic energy-dispersive X-ray spectroscopic analysis after 28 days in Hank’s balanced salt solution (HBSS). The data were analyzed using 2-way analysis of variance with the Student-Newman-Keuls test (P < .05).
MTA Flow showed similar alkalizing activity to that of MTA Angelus. In the solubility test, both materials presented lower values without statistical differences. Both materials showed a marked alkalinizing activity within 3 hours, which continued for 168 hours. MTA Angelus showed statistically higher radiopacity values (P < .05). All materials showed the ability to nucleate calcium phosphate on their surface after 28 days in HBSS.
MTA Flow showed remarkable alkalinizing capability, low solubility, good radiopacity, and the ability to form calcium phosphate deposits after being soaked in simulated body fluid, showing values similar to those of MTA Angelus.
•Chemical-physical properties and apatite-forming ability of MTA Flow were investigated in comparison with MTA Angelus.•MTA Angelus showed statistically higher radiopacity values (P < .05).•Both materials showed the ability to nucleate calcium phosphate on their surface after 28 days in Hank’s balanced salt solution.•MTA Flow showed remarkable alkalinizing capability and low solubility.
The aim of this study was to evaluate some of the physicochemical properties of a new root canal sealer.
The sealers tested were Sealer Plus compared with AH Plus. For the radiopacity, flow, ...solubility, and fabrication of test specimens relative to setting times, the American National Standards Institute/American Dental Association No. 57 (2000) and International Organization for Standardization 6876 (2012) specifications were followed. To measure the initial and final setting times, the ASTM C266/2008 standard was used. pH was evaluated in the time intervals of 3, 24, 72, and 168 hours. Statistical tests were applied to the results obtained at a level of significance of 5%.
The results demonstrated that the Sealer Plus sealer showed a lower radiopacity value than AH Plus sealer (P < .05); however, this was higher than the minimum value recommended by the specifications (ie, 3 mm Al). Relative to flow, the value for Sealer Plus was 19.19 mm and for AH Plus, 19.81 mm (P > .05). Sealer Plus presented initial and final setting times of 138 minutes and 210 minutes, respectively, whereas the values for AH Plus were 437 minutes and 849 minutes, respectively (P < .05). Relative to solubility, Sealer Plus presented 0.21% and AH Plus, 0.27% (P > .05). None of the sealers showed a significant increase in pH (P > .05).
Sealer Plus sealer presented physicochemical properties in accordance with American National Standards Institute/American Dental Association (2000) No. 57 and International Organization for Standardization 6876 (2012) specifications.
•New root canal sealer were introduced on the market, such as Sealer Plus sealer.•There are no studies in the specific literature about the physicochemical properties of the cited sealer.•Sealer Plus presented physicochemical properties in accordance with the specifications No. 57 of ANSI/ADA and ISO 6876.
Accidental sodium hypochlorite extrusion can occur during endodontic irrigation procedures. The symptoms appear immediately and include severe pain, swelling, and probable tissue necrosis adjacent to ...the root of the treated tooth. Treatment of sodium hypochlorite extrusion involves immediate and copious saline irrigation to neutralize the area and prescription of analgesic, anti-inflammatory, and antibiotic medications. Low-level laser therapy (LLLT) might be useful as an adjuvant treatment for damaged soft tissues to improve healing. This case report describes the treatment of accidental extrusion of 1% sodium hypochlorite through a root canal perforation in a mandibular incisor during endodontic treatment. The extrusion caused minor swelling within the mentolabial sulcus and a large area of necrosis in the gingivolabial mucosa and right edentulous premolar area. Conventional treatment was performed in association with LLLT. Clinical and radiographic examinations after 6 months showed complete healing of the necrotic area without paresthesia and the repair of apical tissues.
Posttraumatic stress disorder (PTSD) is characterized by a hypermnesia of the trauma and by a memory impairment that decreases the ability to restrict fear to the appropriate context. Infusion of ...glucocorticoids in the hippocampus after fear conditioning induces PTSD-like memory impairments and an altered pattern of neural activation in the hippocampal-amygdalar circuit. Mice become unable to identify the context as the correct predictor of the threat and show fear responses to a discrete cue not predicting the threat in normal conditions. These data demonstrate PTSD-like memory impairments in rodents and identify a potential pathophysiological mechanism of this condition.
In cancer, the tumour suppressor gene TP53 undergoes frequent missense mutations that endow mutant p53 proteins with oncogenic properties. Until now, a universal mutant p53 gain-of-function program ...has not been defined. By means of multi-omics: proteome, DNA interactome (chromatin immunoprecipitation followed by sequencing) and transcriptome (RNA sequencing/microarray) analyses, we identified the proteasome machinery as a common target of p53 missense mutants. The mutant p53-proteasome axis globally affects protein homeostasis, inhibiting multiple tumour-suppressive pathways, including the anti-oncogenic KSRP-microRNA pathway. In cancer cells, p53 missense mutants cooperate with Nrf2 (NFE2L2) to activate proteasome gene transcription, resulting in resistance to the proteasome inhibitor carfilzomib. Combining the mutant p53-inactivating agent APR-246 (PRIMA-1MET) with the proteasome inhibitor carfilzomib is effective in overcoming chemoresistance in triple-negative breast cancer cells, creating a therapeutic opportunity for treatment of solid tumours and metastasis with mutant p53.
Diarrhea is a prevalent pathological condition frequently associated to the colonization of the small intestine by enterotoxigenic Escherichia coli (ETEC) strains, known to be endemic in developing ...countries. These strains can produce two enterotoxins associated with the manifestation of clinical symptoms that can be used to detect these pathogens. Although several detection tests have been developed, minimally equipped laboratories are still in need of simple and cost-effective methods. With the aim to contribute to the development of such diagnostic approaches, we describe here two mouse hybridoma-derived single chain fragment variable (scFv) that were produced in E. coli against enterotoxins of ETEC strains.
Recombinant scFv were developed against ETEC heat-labile toxin (LT) and heat-stable toxin (ST), from previously isolated hybridoma clones. This work reports their design, construction, molecular and functional characterization against LT and ST toxins. Both antibody fragments were able to recognize the cell-interacting toxins by immunofluorescence, the purified toxins by ELISA and also LT-, ST- and LT/ST-producing ETEC strains.
The developed recombinant scFvs against LT and ST constitute promising starting point for simple and cost-effective ETEC diagnosis.
During the pandemic of COVID-19, numerous waves of infections affected the two hemispheres with different impacts on each country. Throughout these waves, and with the emergence of new variants, ...health systems and scientists have tried to provide real-time responses to the complex biology of SARS-CoV-2, dealing with different clinical presentations, biological characteristics, and clinical impact of these variants. In this context, knowing the extent period in which an infected individual releases infectious viral particles has important implications for public health. This work aimed to investigate viral RNA shedding and infectivity of SARS-CoV-2 beyond 10 days after symptom onset (SO). A prospective multicenter study was performed between July/2021 and February/2022 on 116 immunized strategic personnel with COVID-19 diagnosed by RT-qPCR, with asymptomatic (7%), mild (91%) or moderate disease (2%). At the time of diagnosis, 70% had 2 doses of vaccines, 26% had 2 plus a booster, and 4% had one dose. After day 10 from SO, sequential nasopharyngeal swabs were taken to perform RT-qPCR, viral isolation, and S gene sequencing when possible. Viral sequences were obtained in 98 samples: 43% were Delta, 16% Lambda, 15% Gamma, 25% Omicron (BA.1) and 1% Non-VOC/VOI, in accordance with the main circulating variants at each moment. SARS-CoV-2 RNA was detected 10 days post SO in 57% of the subjects. Omicron was significantly less persistent. Noteworthy, infective viruses could not be isolated in any of the samples. In conclusion, a 10-days isolation period was useful to prevent further infections, and proved valid for the variants studied. Recently, even shorter periods have been applied, as the Omicron variant is prevalent, and worldwide population is largely vaccinated. In the future, facing the possible emergence of new variants and considering immunological status, a return to 10 days may be necessary.
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