222 human prostatic biopsies were used to prepare cell cultures by means of a medium — colony formation permissive — containing fetal calf serum, called TV1. After 7, 14 and 21 days, the cultures ...were examined by optical and scanning electron microscopy. TV1 medium induces the formation and growth of two types of colonies, one mainly composed of epithelioid cells and distinguished by early growth; the second one made up exclusively of fibroblastoid cells which appear later in the culture. Epithelioid colonies, comprising three different cell types, appear to be arranged as a growth halo concentric to the bioptic fragment with a large central area, formed by a monolayer, and a pluristratified edge. Fibroblastoid cells weakly adhere to the substrate and form “satellite growth halos” separated from the primitive bioptic fragment. All the epithelioid cells were positive to cytokeratin LP34 Mab and negative to anti-smooth muscle-actin and anti-pro-line-4-hydroxylase antibodies. Fibroblastoid cells were only anti-proline-4-hydroxylase positive. The cell kinetics of epithelioid cells were also studied, revealing an extension of the S phase, in contrast to what happened with WAJC 404, and consequently a reduction of the percentage of cells entering mitosis. For this reason, the addition of fetal serum to the culture medium does not allow the use of prostate primary cultures for more than 14 days.
This work studies the effects of dihydrotestosterone (DHT) and epidermal growth factor (EGF) on the growth, morphology and phenotype characterisation of the U285 line obtained from human prostate ...hyperplastic tissue. Modifications of growth rate induced by these two substances have been evaluated by means of the neutral red assay formulated by Borenfreund and Puerner (1985) as well as by means of Kenacid blue assay described by Knox et al. (1986), culturing cells for 24, 48 and 72 hr with scalar doses of DHT (0.5, 1, 2, 5, 10 μM) and EGF (5, 10, 20, 100 ng/ml). An optical microscope connected to a computer aided system and a scanning electron microscope were used to monitor morphological changes induced by DHT and EGF. The immunophenotype characterisation of the treated and control cells was carried out by using a monoclonal antibody panel. Our results show that the expression of anti-cytokeratin 5+6+18, anticytokeratin 8+18+19 and anti-proline-4-hydroxylase antibodies varied in relation to the type of treatment undergone by the cells. Moreover, exogenous DHT does provoke a flattening of the U285 cells without modifying their rate of growth, while EGF both shortens the lag phase reactivating the quiescent cells and regulates the subsequent log growth phase, thus causing no cellular overgrowth.
Putative avocado toxicity in two dogs Buoro, I B; Nyamwange, S B; Chai, D ...
Onderstepoort journal of veterinary research
61, Številka:
1
Journal Article
Recenzirano
Two dogs were seen at the University Veterinary Teaching Hospital, Nairobi, Kenya, both having histories of dyspnoea, progressively enlarging abdomens, anasarca, ascites, pleural and pericardial ...effusion, and pulmonary oedema. One of the dogs had a mild neutrophilic leucocytosis, elevated levels of alkaline phosphatase, alanine aminotransferase, lactate dehydrogenase and proteinuria. Histopathological examination of the myocardium revealed some damage to myocytes and a mononuclear cellular infiltration involving the myocardium, liver and kidneys. The two dogs had a fondness for avocado fruits and, as the presenting syndrome is identical to that seen in goats, sheep and horses poisoned by avocados, a comparison is made and the probable manifestation of this poisoning presented.
Regards sur 40 ans de problèmes inverses en hydrogéologie de Marsily, Ghislain; Delhomme, Jean-Pierre; Delay, Frédéric ...
Comptes rendus de l'Académie des sciences. Série II. Fascicule a, Sciences de la terre et des planètes,
1999, Letnik:
329, Številka:
2
Journal Article
We review the main stages of the evolution of ideas and methods for solving the ‘inverse problem’ in hydrogeology, i.e. the identification of the permeability field in single phase flow on the basis ...of piezometric data, in mainly steady-slate conditions.
On passe en revue les étapes majeures de l'évolution des idées et des méthodes de résolution du ≪ problème inverse ≫ en hydrogéologie, c'est-à-dire de l'identification du champ des perméabilités en écoulement monophasique, à partir des informations piézométriques, principalement en régime; permanent.
