Summary
Arthrocentesis has an effect of washing out inflammatory products that accumulate in the joint compartment of a dysfunctional temporomandibular joint (TMJ). The procedure removes inflammatory ...cytokines, which are pain‐causing substances, for early reduction of TMJ pain and quick recovery of jaw function, thus increasing the possibility of a successful rehabilitation. The aim of this study was to investigate the relationship between arthroscopy synovitis grade in patients with unilateral high condylar fractures and concentrations of the pro‐inflammatory cytokines tumour necrosis factor (TNF)‐alpha as well as of matrix metalloproteinases (MMPs) in washed‐out synovial fluid (SF) samples obtained from those patients. A total of 26 patients with unilateral high condylar fractures who underwent arthrocentesis for a therapeutic purpose were examined. SF samples were collected before performing arthroscopy to determine synovitis grade. The detection rates and concentrations of TNF‐alpha and MMPs were determined, and their association with synovitis grade was analysed. TNF‐alpha was detected in 23 and MMP‐3 in 22 of the TMJs. There was a correlation between synovitis grade and concentration of TNF‐alpha in the fracture group. Furthermore, the concentrations of TNF‐alpha and MMP‐3 were significantly higher as compared to the control group, comprised of TMJs on the non‐fracture side of the same patients, while a correlation was also noted between TNF‐alpha concentration and synovitis grade in the fracture group. The present findings may provide a biological/biochemical rationale for arthrocentesis as a reasonable treatment modality for high condylar fractures.
It has been reported that not only selective alveolar-bone resorption, but also receptor activator of nuclear factor kappa B ligand (RANKL) expression is induced on the compressed side of an ...orthodontically moving tooth. Numerous reports have described the pharmacological acceleration of tooth movement (TM) through the activation of osteoclasts. However, because of rapid flush out by blood circulation, daily systemic administration or daily local injection is needed. Previously, we discovered that every-3-days OPG gene transfer to the periodontal-tissue inhibited RANKL-mediated osteoclastogenesis and diminished experimental TM. Therefore, we hypothesized that local RANKL gene transfer into the periodontal tissue would accelerate TM. The upper first molars of 6-week-old male Wistar rats were moved palatally using fixed orthodontic wires. The inactivated hemagglutinating-virus of Japan (HVJ) envelope vector containing the mouse RANKL expression plasmid was injected periodically into the palatal periodontal tissue of the upper first molars during TM. Local RANKL gene transfer significantly enhanced RANKL expression and osteoclastogenesis in periodontal tissue without any systemic effects. The TM rate was significantly increased in the RANKL gene transfer side. In conclusion, we demonstrated that transfer of the RANKL gene to the periodontal-tissue activated osteoclastogenesis and accelerated the amount of experimental TM. Local RANKL gene transfer might be a useful tool not only for shortening orthodontic treatment, but also for moving ankylosed teeth where teeth, fuse to the surrounding bone.
Experimental studies have been conducted to clarify the influence of horizontal harmonic excitations on the dynamic stability of a slender cantilever beam under vertical harmonic excitation. Three ...kinds of aluminum test beams with rectangular cross section have been used. The test beam being clamped at one end and free at the other end, was vertically stood, and was harmonically excited to both vertical and horizontal directions simultaneously. The direction of the horizontal excitation was taken parallel to one of the beam side faces, i.e. two directions were considered as
X
and
Y
directions which have the largest and smallest flexural rigidity, respectively. By varying the horizontal excitation amplitude, keeping the amplitude of excitation in the vertical direction, the influence of the horizontal excitation has been investigated on the principal instability regions in which unstable vibration of the fundamental vibration mode occurs. The excitation frequency in the vertical excitation was taken around twice the fundamental natural frequency 2
f
Y
1
in smallest rigidity direction, while that in the horizontal direction was taken around both the fundamental natural frequency
f
Y
1
and twice of it 2
f
Y
1
. Obtained experimental results present useful fundamental data for aseismatic design of structures under earthquake containing both vertical and horizontal excitation components.
The dynamic stability of a vertically standing cantilevered beam simultaneously excited in both horizontal and vertical directions at its base is studied theoretically. The beam is assumed to be an ...inextensible Euler–Bernoulli beam. The governing equation of motion is derived using Hamilton's principle and has a nonlinear elastic term and a nonlinear inertia term. A forced horizontal external term is added to the parametrically excited system. Applying Galerkin's method for the first bending mode, the forced Mathieu–Duffing equation is derived. The frequency response is obtained by the harmonic balance method, and its stability is investigated using the phase plane method. Excitation frequencies in the horizontal and vertical directions are taken as 1:2, from which we can investigate the influence of the forced response under horizontal excitation on the parametric instability region under vertical excitation. Three criteria for the instability boundary are proposed. The influences of nonlinearities and damping of the beam on the frequency response and parametric instability region are also investigated. The present analytical results for instability boundaries are compared with those of experiments carried out by one of the authors.
•Non-linear response and stability of beam under both horizontal and vertical excitations.•Non-linear inertia and non-linear elastic terms considered.•Effects of non-linear terms and damping parameter on beam response and stability region.
After having announced the statistically significant observation (5.6σ) of the new exotic πK atom, the DIRAC experiment at the CERN proton synchrotron presents the measurement of the corresponding ...atom lifetime, based on the full πK data sample: τ=(5.5−2.8+5.0)×10−15 s. By means of a precise relation (≈1%) between atom lifetime and scattering length, the following value for the S-wave isospin-odd πK scattering length a0−=13(a1/2−a3/2) has been derived: |a0−|=(0.072−0.020+0.031)Mπ−1.
The observation of hydrogenlike πK atoms, consisting of π^{-}K^{+} or π^{+}K^{-} mesons, is presented. The atoms are produced by 24 GeV/c protons from the CERN PS accelerator, interacting with ...platinum or nickel foil targets. The breakup (ionization) of πK atoms in the same targets yields characteristic πK pairs, called "atomic pairs," with small relative momenta Q in the pair center-of-mass system. The upgraded DIRAC experiment observed 349±62 such atomic πK pairs, corresponding to a signal of 5.6 standard deviations. This is the first statistically significant observation of the strange dimesonic πK atom.
The adapted DIRAC experiment at the CERN PS accelerator observed for the first time long-lived hydrogenlike π^{+}π^{-} atoms, produced by protons hitting a beryllium target. A part of these atoms ...crossed the gap of 96 mm between the target and a 2.1 μm thick platinum foil, in which most of them dissociated. Analyzing the observed number of atomic pairs, n_{A}^{L}=436_{-61}^{+157}|_{tot}, the lifetime of the 2p state is found to be τ_{2p}=(0.45_{-0.30}^{+1.08}|_{tot})×10^{-11} s, not contradicting the corresponding QED 2p state lifetime τ_{2p}^{QED}=1.17×10^{-11} s. This lifetime value is three orders of magnitude larger than our previously measured value of the π^{+}π^{-} atom ground state lifetime τ=(3.15_{-0.26}^{+0.28}|_{tot})×10^{-15} s. Further studies of long-lived π^{+}π^{-} atoms will allow us to measure energy differences between p and s atomic states and so to discriminate between the isoscalar and isotensor ππ scattering lengths with the aim to check QCD predictions.
Periodontal remodeling during orthodontic tooth movement is a result of mechanical stresses. The application of excessive orthodontic force induces cell death. However, the nature of compressive ...force-induced cell death is unclear. We examined whether the in vitro application of continuous compressive force would induce apoptosis in human osteoblast-like cells (MG-63 cells), and investigated the mechanism by which apoptosis was initiated. The cells became aligned irregularly, and cell viability decreased, indicating that the compressive force caused cell death. According to the TUNEL analysis, the number of apoptotic cells increased significantly in a time-and force-dependent manner. Caspase-3 activity increased with the magnitude of the compressive force, and this effect was reduced significantly by a caspase-8 inhibitor, whereas a caspase-9 inhibitor had no such effect. We conclude that the in vitro application of compressive force can induce apoptosis in MG-63 cells through the activation of caspase-3 via the caspase-8 signaling cascade.
The results of a search for hydrogen-like atoms consisting of π∓K± mesons are presented. Evidence for πK atom production by 24 GeV/c protons from CERN PS interacting with a nickel target has been ...seen in terms of characteristic πK pairs from their breakup in the same target (178±49) as well as in terms of produced πK atoms (653±42). Using these results, the analysis yields a first value for the πK atom lifetime of τ=(2.5−1.8+3.0) fs and a first measurement of the S-wave isospin-odd πK scattering length |a0−|=13|a1/2−a3/2|=(0.11−0.04+0.09)Mπ−1 (aI for isospin I).
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