Mesenchymal stem cell-derived exosomes (MSC-exos), with its inherent capacity to modulate cellular behavior, are emerging as a novel cell-free therapy for bone regeneration. Herein, focusing on ...practical applying problems, the osteoinductivity of MSC-exos produced by different stem cell sources (rBMSCs/rASCs) and culture conditions (osteoinductive/common) were systematically compared to screen out an optimized osteogenic exosome (BMSC-OI-exo). Via bioinformatic analyses by miRNA microarray and in vitro pathway verification by gene silencing and miRNA transfection, we first revealed that the osteoinductivity of BMSC-OI-exo was attributed to multi-component exosomal miRNAs (let-7a-5p, let-7c-5p, miR-328a-5p and miR-31a-5p). These miRNAs targeted Acvr2b/Acvr1 and regulated the competitive balance of Bmpr2/Acvr2b toward Bmpr-elicited Smad1/5/9 phosphorylation. On these bases, lyophilized delivery of BMSC-OI-exo on hierarchical mesoporous bioactive glass (MBG) scaffold was developed to realize bioactivity maintenance and sustained release by entrapment in the surface microporosity of the scaffold. In a rat cranial defect model, the loading of BMSC-OI-exo efficiently enhanced the bone forming capacity of the scaffold and induced rapid initiation of bone regeneration. This paper could provide empirical bases of MSC-exo-based therapy for bone regeneration and theoretical bases of MSC-exo-induced osteogenesis mechanism. The BMSC-OI-exo-loaded MBG scaffold developed here represented a promising bone repairing strategy for future clinical application.
Initial Observations by the GOLD Mission Eastes, R. W.; McClintock, W. E.; Burns, A. G. ...
Journal of geophysical research. Space physics,
July 2020, Letnik:
125, Številka:
7
Journal Article
Recenzirano
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The NASA Global‐scale Observations of the Limb and Disk (GOLD) mission has flown an ultraviolet‐imaging spectrograph on SES‐14, a communications satellite in geostationary orbit at 47.5°W longitude. ...That instrument observes the Earth's far ultraviolet (FUV) airglow at ~134–162 nm using two identical channels. The observations performed include limb scans, stellar occultations, and images of the sunlit and nightside disk from 6:10 to 00:40 universal time each day. Initial analyses reveal interesting and unexpected results as well as the potential for further studies of the Earth's thermosphere‐ionosphere system and its responses to solar‐geomagnetic forcing and atmospheric dynamics. Thermospheric composition ratios for major constituents, O and N2, temperatures near 160 km, and exospheric temperatures are retrieved from the daytime observations. Molecular oxygen (O2) densities are measured using stellar occultations. At night, emission from radiative recombination in the ionospheric F region is used to quantify ionospheric density variations in the equatorial ionization anomaly (EIA). Regions of depleted F region electron density are frequently evident, even during the current solar minimum. These depletions are caused by the “plasma fountain effect” and are associated with the instabilities, scintillations, or “spread F” seen in other types of observations, and GOLD makes unique observations for their study.
Plain Language Summary
The NASA Global‐scale Observations of the Limb and Disk (GOLD) mission has flown a dual‐channel, ultraviolet‐imaging spectrograph on SES‐14, a communications satellite in geostationary orbit at 47.5°W longitude. That instrument observes the Earth's far ultraviolet (FUV) airglow at ~134–162 nm. The observations performed include images of the Earth's sunlit and nightside disk, limb scans, and stellar occultations, from 6:10 to 00:40 universal time each day. Initial analyses reveal interesting and unexpected results as well as the potential for further studies of the Earth's thermosphere‐ionosphere system and its responses to solar‐geomagnetic forcing and atmospheric dynamics. Thermospheric temperatures and composition ratios for major constituents, O and N2, near 160‐km altitude and exospheric temperatures are retrieved from the daytime observations. Molecular oxygen (O2) densities are measured using stellar occultations. At night, emission from radiative recombination in the ionospheric F region is used to quantify ionospheric density variations in the equatorial ionization anomaly (EIA). Regions of depleted F region electron density are frequently evident in the EIA, even during the current solar minimum.
Key Points
GOLD makes global‐scale, synoptic measurements of the temperature, composition, and densities in the thermosphere‐ionosphere system
Most measurements by the GOLD instrument are made in one of four modes
The observations are providing new and surprising insights into the characteristics and behavior of the thermosphere and ionosphere
•MnCo2O4 nanowire array is prepared by a fast and facile hydrothermal method.•MnCo2O4 nanowire array exhibits noticeable pseudocapacitive properties.•The as-prepared nanowire array is also a ...promising material for Li-ion batteries.
One-dimension MnCo2O4 nanowire arrays are synthesized on nickel foam by a facile hydrothermal method. The MnCo2O4 nanowires are highly crystalline with an average diameter of 70nm and exhibit excellent properties for electrochemical energy storage. Impressively, the MnCo2O4 nanowire array exhibits noticeable pseudocapacitive performance with a high capacitance of 349.8 F g−1 at 1 A g−1 and 328.9 F g−1 at 20 A g−1 as well as excellent cycling stability. As an anode material for Li-ion batteries, the MnCo2O4 nanowire array delivers an initial specific discharge capacity of 1288.6 mAh g−1 at 100mAg−1, with reversible capacity retention of 92.7% after 50 cycles. The outstanding electrochemical performances are mainly attributed to its nanowire array architecture which provides large reaction surface area, fast ion and electron transfer and good structure stability.
The WO3/PANI core/shell nanowire array is prepared by the combination of solvothermal and electropolymerization methods. The core/shell nanowire array film shows remarkable enhancement of the ...electrochromic properties. In particular, a significant optical modulation (59% at 700nm), fast switching speed, high coloration efficiency (86.3cm2C−1 at 700nm) and excellent cycling stability are achieved for the core/shell nanowire array film. The improved electrochromic properties are mainly attributed to the formation of the donor–acceptor system, and the porous space among the nanowires, which can make fast ion diffusion and provide larger surface area for charge-transfer reactions. The data indicate great promise for the WO3/PANI core/shell nanowire array as a potential multicolor electrochromic material.
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•The WO3/PANI core/shell nanowire array is successfully prepared.•The dual-electrochromism effect is obtained for the core/shell nanowire array.•The core/shell structure exhibits large optical modulation and fast switching speed.•High CE and excellent cycling stability are achieved for the core/shell structure.
Silage making can be conveniently divided into field, ensiling, storage, and feed-out phases. In all of these stages, controllable and uncontrollable components can affect silage quality. For ...instance, silages produced in hot or cold regions are strongly influenced by uncontrollable climate-related factors. In hot regions, crops for silage are influenced by (1) high temperatures negatively affecting corn yield (whole-crop and grain) and nutritive value, (2) butyric and alcoholic fermentations in warm-season grasses (Panicum, Brachiaria, and Pennisetum genera) and sugarcane, respectively, and (3) accelerated aerobic deterioration of silages. Ensiling expertise and economic factors that limit mechanization also impair silage production and utilization in hot environments. In cold regions, a short and cool growing season often limits the use of crops sensitive to cool temperature, such as corn. The fermentation triggered by epiphytic and inoculated microorganisms can also be functionally impaired at lower temperature. Although the use of silage inoculants has increased in Northern Europe, acid-based additives are still a good option in difficult weather conditions to ensure good fermentation quality, nutritive value, and high intake potential of silages. Acid-based additives have enhanced the quality of round bale silage, which has become a common method of forage preservation in Northern Europe. Although all abiotic factors can affect silage quality, the ambient temperature is a factor that influences all stages of silage making from production in the field to utilization at the feed bunk. This review identifies challenges and obstacles to producing silages under hot and cold conditions and discusses strategies for addressing these challenges.
Defects of either articular cartilage or subchondral bone would destroy the structural integrity and functionality of the joint. Reconstruction of osteochondral defects requires difunctional ...scaffolds that simultaneously induce cartilage and subchondral bone morphogenesis, however, high-performance cartilage reconstructive scaffolds remain a considerable challenge. In this study, a solvent-free urethane crosslinking and spontaneous pore-forming procedure under room temperature was proposed and optimized to produce PEGylated poly(glycerol sebacate) (PEGS) scaffolds with controllable crosslinking degrees and hierarchical macro-/micro-porosities. Based on the economical and feasible preparative approach, the viscoelastic PEGS-12h with low crosslinking degree was demonstrated to significantly stimulate chondrogenic differentiation, maintain chondrocyte phenotype and enhance cartilage matrix secretion compared to elastic polymer with high crosslinking degree, emphasizing the importance of matrix viscoelasticity in cartilage regeneration. On this basis, the viscoelastic low-crosslinked PEGS-12h was combined with the well-acknowledged osteoinductive mesoporous bioactive glass (MBG) to construct a difunctional PEGS/MBG bilayer scaffold, and evaluated in a full-thickness osteochondral defect model in vivo. The PEGS/MBG bilayer scaffold successfully reconstructed well-integrated articular hyaline cartilage and its subchondral bone in 12 weeks, exhibiting extraordinary regenerative efficiency. The results indicated that the viscoelastic PEGS scaffold and PEGS/MBG bilayer scaffold proposed in this study made an excellent candidate for cartilage and osteochondral regeneration, and was expected for clinical translation in the future.
The complicated structure of the neutron cannot be calculated using first-principles calculations due to the large colour charge of quarks and the self-interaction of gluons. Its simplest structure ...observables are the electromagnetic form factors1, which probe our understanding of the strong interaction. Until now, a small amount of data has been available for the determination of the neutron structure from the time-like kinematical range. Here we present measurements of the Born cross section of electron–positron annihilation reactions into a neutron and anti-neutron pair, and determine the neutron’s effective form factor. The data were recorded with the BESIII experiment at centre-of-mass energies between 2.00 and 3.08 GeV using an integrated luminosity of 647.9 pb−1. Our results improve the statistics on the neutron form factor by more than a factor of 60 over previous measurements, demonstrating that the neutron form factor data from annihilation in the time-like regime is on par with that from electron scattering experiments. The effective form factor of the neutron shows a periodic behaviour, similar to earlier observations of the proton form factor. Future works—both theoretical and experimental—will help illuminate the origin of this oscillation of the electromagnetic structure observables of the nucleon.Form factors encode the structure of nucleons. Measurements from electron–positron annihilation at BESIII reveal an oscillating behaviour of the neutron electromagnetic form factor, and clarify a long-standing photon–nucleon interaction puzzle.
The cross section for the process e^{+}e^{-}→π^{+}π^{-}J/ψ is measured precisely at center-of-mass energies from 3.77 to 4.60 GeV using 9 fb^{-1} of data collected with the BESIII detector operating ...at the BEPCII storage ring. Two resonant structures are observed in a fit to the cross section. The first resonance has a mass of (4222.0±3.1±1.4) MeV/c^{2} and a width of (44.1±4.3±2.0) MeV, while the second one has a mass of (4320.0±10.4±7.0) MeV/c^{2} and a width of (101.4_{-19.7}^{+25.3}±10.2) MeV, where the first errors are statistical and second ones are systematic. The first resonance agrees with the Y(4260) resonance reported by previous experiments. The precision of its resonant parameters is improved significantly. The second resonance is observed in e^{+}e^{-}→π^{+}π^{-}J/ψ for the first time. The statistical significance of this resonance is estimated to be larger than 7.6σ. The mass and width of the second resonance agree with the Y(4360) resonance reported by the BABAR and Belle experiments within errors. Finally, the Y(4008) resonance previously observed by the Belle experiment is not confirmed in the description of the BESIII data.
Vertically aligned hierarchical WO3 nano-architectures on transparent conducting substrate (3×2.5cm2 in size and sheet resistance Rs=10Ω) are produced via a template-free solvothermal method. The ...nanostructured array films with thicknesses of about 1.1μm show remarkable enhancement of the electrochromic properties in visible spectrum and infrared region. In particular, a significant optical modulation (66.5% and 66.0% at 633nm, 73.8% and 53.9% at 2000nm, 57.7% and 51.7% at 8μm), fast switching speed (4.6s/3.6s and 2.0s/3.4s), high coloration efficiency (126 and 120cm2C−1 at 633nm) and excellent cycling stability (maintained 77.5% and 81.7% of the initial optical modulation after 4500-cycles) are achieved for the nanotree and nanowire arrays, respectively. The improved electrochromic properties are mainly attributed to the vertically aligned structure and the porous space among the nanotrees or nanowires, which make the diffusion of H+ in these arrays easier and also supply larger surface area for charge-transfer reactions.
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•Vertically aligned WO3 nano-architectures are produced via a solvothermal method.•WO3 nano-architectures exhibit significant optical modulation and fast switching speed.•High coloration efficiency and excellent cycling performance are achieved for the nano-architectures.
Though immensely successful, the standard model of particle physics does not offer any explanation as to why our Universe contains so much more matter than antimatter. A key to a dynamically ...generated matter-antimatter asymmetry is the existence of processes that violate the combined charge conjugation and parity (CP) symmetry
. As such, precision tests of CP symmetry may be used to search for physics beyond the standard model. However, hadrons decay through an interplay of strong and weak processes, quantified in terms of relative phases between the amplitudes. Although previous experiments constructed CP observables that depend on both strong and weak phases, we present an approach where sequential two-body decays of entangled multi-strange baryon-antibaryon pairs provide a separation between these phases. Our method, exploiting spin entanglement between the double-strange Ξ
baryon and its antiparticle
Formula: see text, has enabled a direct determination of the weak-phase difference, (ξ
- ξ
) = (1.2 ± 3.4 ± 0.8) × 10
rad. Furthermore, three independent CP observables can be constructed from our measured parameters. The precision in the estimated parameters for a given data sample size is several orders of magnitude greater than achieved with previous methods
. Finally, we provide an independent measurement of the recently debated Λ decay parameter α
(refs.
). The Formula: see text asymmetry is in agreement with and compatible in precision to the most precise previous measurement
.
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