The self-assembly formation of Zn/ZnO treelike nanostructures was achieved by simply aging the corresponding stable colloidal solution, produced by laser ablation in liquid, at room temperature ...without any further heating treatment. The self-assembled treelike structures consist of oriented attached nanoparticles with small misorientations and greatly improved crystallinity and exhibit significantly enhanced visible emission with two peaks in the blue and green regions, accompanying the decrease of the plasma resonance absorption of the core-part Zn nanocrystal. The self-assembly mechanism was attributed to the imperfect oriented attachment. The small misorientations among the attached nanoparticles were observed, and their accumulation guided the self-assembly formation of the multibranched nanostructures. As compensation for the insufficient driving force, adequate colloid stability and aging time were crucial for the accomplishment of the self-assembly process. This work could deepen the understanding of the nanocrystal self-assembly behavior, especially in the very “soft” environment, and the effect of the self-assembly process on the optical properties.
A novel label-free impedimetric immunosensor for the quantitative detection of aflatoxin B 1 (AFB 1) was developed based on orientated staphylococcus protein A (SPA) modified gold three dimensional ...nanotube ensembles (3DTNEEs) which consisted of gold nanotubes fabricated by combining the technologies of template synthesis and controlled chemical etching of track-etched polycarbonate membrane. The structure and morphology of 3DTNEEs were evaluated by scanning electron microscopy. The diffusion characteristic and local current distribution on 3DTNEEs were simulated via the COMSOL Multiphysics 4.4 simulative software. The SPA self-assembly modified 3DTNEEs (SPA/3DTNEEs) was assessed by X-ray photoelectron spectroscopy. Electrochemical impedance spectroscopy was employed to characterize the fabrication process and optimize working conditions. The model of the molecular recognition interface was constructed and analyzed. The detection limit of AFB1 was 1.0 ... 10 -12 g mL -1 (S/N = 3) with a linear AFB 1 concentration range of 4.0 ... 10 -12 to 6.0 ... 10 -9 g mL -1. The AFB 1 recovery test in corn samples demonstrated one potential application of the immunosensor.
The IκB kinase (IKK) complex has been found to have critical functions in cancer and the immune system. In particular, IKKα, which is a member of the IKK complex, has been shown to influence the ...inflammatory response and malignant diseases. However, the role of IKKα in macrophages after myocardial infarction (MI) remains largely unknown.
Sham or MI operations were performed on macrophage-specific IKKɑ knockout (mIKKɑ−/−) mice and IKKɑflox/flox littermates. We ligated the left anterior descending coronary artery of the MI group and observed the results at 3, 7, and 30 days after MI.
We discovered more severe cardiac dysfunction with reduced angiogenesis, fibrosis, and collagen deposition in mIKKɑ−/− than in IKKɑflox/flox. In addition, we also observed that macrophages in mIKKɑ−/− were easier to polarize to the M1 phenotype and expressed more proinflammatory factors than IKKɑflox/flox. Mechanistically, IKKα deficiency in macrophages inhibited the alternative nuclear factor-κB/RelB pathway and enhanced the MEK1/2/ERK1/2 pathway.
Overall, our data identified IKKɑ in the heart as a novel mediator that protected the heart from a severe inflammatory response and attenuated ventricular remodelling after MI by negatively regulating macrophage polarization to the M1 phenotype. Therefore, IKKα may serve as a potential therapeutic target for treatment after MI.
Il a été montré que le complexe IκB kinase (IKK) assure des fonctions essentielles dans le cancer et le système immunitaire. Plus précisément, l’IKKα, qui fait partie du complexe IKK, s’est avérée avoir une influence sur la réponse inflammatoire et les affections malignes. Le rôle de l’IKKα dans les macrophages après un infarctus du myocarde (IM) reste néanmoins en grande partie inconnu.
Des chirurgies fictives ou pour un IM ont été pratiquées sur des souris dont le complexe IKKɑ spécifique des macrophages avait été désactivé (mIKKɑ−/−) et sur des congénères IKKɑflox/flox de la même portée. Nous avons ligaturé l’artère coronaire descendante antérieure gauche dans le groupe IM et observé les résultats 3, 7 et 30 jours après l’IM.
Nous avons noté une dysfonction cardiaque plus grave avec réduction de l’angiogenèse, fibrose et dépôts de collagène chez les sujets mIKKɑ−/−, comparativement aux sujets IKKɑflox/flox. Nous avons également noté qu’il était plus facile de polariser les macrophages des souris mIKKɑ−/− vers le phénotype M1, et que celles-ci exprimaient davantage de facteurs pro-inflammatoires, comparativement aux souris IKKɑflox/flox. D’un point de vue mécanistique, la carence en IKKα des macrophages a inhibé la voie de signalisation alternative κB/RelB du facteur nucléaire et accentué la voie MEK1/2/ERK1/2.
D’une manière générale, nos données indiquent que l’IKKɑ présente dans le cœur constitue un médiateur novateur, qui protège le cœur contre la réponse inflammatoire marquée et diminue le remodelage ventriculaire après un IM, en régulant à la baisse la polarisation des macrophages vers le phénotype M1. L’IKKα pourrait donc constituer une cible potentielle du traitement après un IM.
An ultrasensitive label free impedimetric immunosensor for AFB
1
detection was fabricated based on poly(
o
-phenylenediamine) (PoPD) electropolymerized film modified gold three dimensional ...nanoelectrode ensembles (3DNEEs). The nanoelectrode ensembles were fabricated by template synthesis in track etched polycarbonate (PC) membranes. Scanning electron microscopy and X-ray diffraction were used for the evaluation of the properties of 3DNEEs. Anti-AFB
1
was directionally immobilized on
Staphylococcus
protein A (SpA) with orientation functions. Cyclic voltammetry and electrochemical impedance spectroscopy were employed to characterize the fabrication process and optimize working conditions. The interface model of molecular recognition was constructed and reasonably interpreted. The detection limit was 0.019 ng mL
−1
(
S
/
N
= 3). The linear detection concentration range of AFB
1
was from 0.04 to 8.0 ng mL
−1
.
An ultrasensitive label free impedimetric immunosensor for AFB
1
detection was fabricated based on poly(
o
-phenylenediamine) (PoPD) electropolymerized film modified gold three dimensional nanoelectrode ensembles (3DNEEs).
An ultrasensitive label free impedimetric immunosensor for AFB 1 detection was fabricated based on poly( o -phenylenediamine) (PoPD) electropolymerized film modified gold three dimensional ...nanoelectrode ensembles (3DNEEs). The nanoelectrode ensembles were fabricated by template synthesis in track etched polycarbonate (PC) membranes. Scanning electron microscopy and X-ray diffraction were used for the evaluation of the properties of 3DNEEs. Anti-AFB 1 was directionally immobilized on Staphylococcus protein A (SpA) with orientation functions. Cyclic voltammetry and electrochemical impedance spectroscopy were employed to characterize the fabrication process and optimize working conditions. The interface model of molecular recognition was constructed and reasonably interpreted. The detection limit was 0.019 ng mL −1 ( S / N = 3). The linear detection concentration range of AFB 1 was from 0.04 to 8.0 ng mL −1 .
The incidence of occult cervical lymph node metastases (OCLNM) is reported to be 20%-30% in early-stage oral cancer and oropharyngeal cancer. There is a lack of an accurate diagnostic method to ...predict occult lymph node metastasis and to help surgeons make precise treatment decisions.
To construct and evaluate a preoperative diagnostic method to predict occult lymph node metastasis (OCLNM) in early-stage oral and oropharyngeal squamous cell carcinoma (OC and OP SCC) based on deep learning features (DLFs) and radiomics features.
A total of 319 patients diagnosed with early-stage OC or OP SCC were retrospectively enrolled and divided into training, test and external validation sets. Traditional radiomics features and DLFs were extracted from their MRI images. The least absolute shrinkage and selection operator (LASSO) analysis was employed to identify the most valuable features. Prediction models for OCLNM were developed using radiomics features and DLFs. The effectiveness of the models and their clinical applicability were evaluated using the area under the curve (AUC), decision curve analysis (DCA) and survival analysis.
Seventeen prediction models were constructed. The Resnet50 deep learning (DL) model based on the combination of radiomics and DL features achieves the optimal performance, with AUC values of 0.928 (95% CI: 0.881-0.975), 0.878 (95% CI: 0.766-0.990), 0.796 (95% CI: 0.666-0.927) and 0.834 (95% CI: 0.721-0.947) in the training, test, external validation set1 and external validation set2, respectively. Moreover, the Resnet50 model has great prediction value of prognosis in patients with early-stage OC and OP SCC.
The proposed MRI-based Resnet50 deep learning model demonstrated high capability in diagnosis of OCLNM and prognosis prediction in the early-stage OC and OP SCC. The Resnet50 model could help refine the clinical diagnosis and treatment of the early-stage OC and OP SCC.
Traditional PHC pipe pile in foundation engineering consumes high energy and has insufficient durability. A magnesium oxide carbonization test block is a new type of environmental protection block ...which bases on activated magnesium oxide cementation technology. The use of CO2 carbonation technology allows reactive magnesia to react to form basic magnesium carbonate to increase the compressive strength and durability of the block. Three kinds of different magnesium oxide powders were subjected to pressure test and determined the key technical parameters, such as optimal raw materials, sample preparation methods, carbonization environment and technology, and optimized design of pipe pile concrete material system.
A new functionalized
l-cysteine surface modified 3D gold brush nanoelectrode assembly BNEE (
l-cys/BNEEs) was prepared. The BNEEs consisted of gold nanowires 100
nm in diameter and up to 400
nm in ...length fabricated by template synthesis in track etched polycarbonate membranes. The nanowires were exposed by controlled chemical etching of the membrane and were then modified by coating
l-cys on the surface of the exposed gold nanowires. The morphology of the BNEEs was imaged by scanning electron microscopy and the real active area of BNEEs was determined by electrochemical impedance spectroscopy. The redox of daunorubicine (DNR) at the
l-cys/BNEEs exhibited absorption-controlled characteristics and higher current activity than that at
l-cys surface modified 2D disk NEEs (
l-cys/DNEEs). The square wave voltammetry technique was employed to detect DNR. The detection limit was 1.0
×
10
−8
M (s/n
=
3). The linear detection concentration range of DNR was from 2.5
×
10
−8 to 4.0
×
10
−7
M.
A label free impedimetric immunosensor for IgG with high sensitivity, fine reproducibility and wider linear range was fabricated via immobilizing goat anti-rabbit Immunoglobulin G (IgG) onto Poly(o- ...phenylenediamine) (PoPD) electropolymerized film modified gold electrode by glutaraldehyde crosslinking. An electrochemical interfacial modeling of biomolecular recognition was constructed and reasonably interpretated. The linear detection concentration ranges of IgG were 0.1~10 ng/mL and 10~100 ng/mL. The detection limit was 0.05 ng/mL(s/n=3). The immunosensor could be reused more than 25 times when renewed by HCl-Glycine buffer solution (pH 2.80).
The 1.6-hexanedithiol (HDT) chemically modified gold (HDT/Au) electrode were prepared by self-assembly. The HDT modified layer blocked the heterogeneous electrolysis of Fe (CN)63-/4- strongly except ...sites of pinholes and defects which behaved like ultramicro electrode ensembles (UMEs). The features of UMEs and the electrochemcial characteristics of Fe(CN)63-/4 at UMEs with different modifying hours were studied by electrochemical impedance spectroscopy. HDT modified gold electrode promoted the electrochemical process for redox reaction of 2,3-diaminophenazine (DAP), the product of OPD-H2O2-HRP enzymatic catalyst reaction system and blocked hydrogen evolution effectively. The detection limit determined by square wave was 10-11g/ml, which is about one order of magnitude lower than the reported lowest results.
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